S3352 400

C57BL/6J (Stock #000664), Il10^GFP (B6.129S6-Il10^tm1Flv/RthsnJ, Stock #008379), Foxp3^RFP (C57BL/6-Foxp3^tm1Flv/J, Stock #008374), Foxp3^creYFP (B6.129(Cg)-Foxp3^{tm4(YFP/icre)Ayr}/J, Stock 016959), and Il10^-/- (B6.129P2-Il10^tm1Cgn/J, Stock #002251) mice, all on the C57BL/6 background, as well as Foxp3^GFP mice on the BALB/c background (C.Cg-Foxp3^tm2Tch, Stock #006769) were purchased from the Jackson Laboratory (Bar Harbor, ME). Il10^fl/fl mice were kindly gifted by Dr. Werner Muller (Roers et al., 2004 (link)) via Dr. Asma Nusrat (Quiros et al., 2017 (link)). Il10^GFP and Foxp3^RFP mice were crossed to make Il10^GFP x Foxp3^RFP dual-reporters, and Il10^fl/fl and Foxp3^cre-YFP were crossed to make FoxP3-specific IL-10 knockouts (IL-10 cKO) in our facility. Mice were housed in a 12 hr light/dark cycle-specific pathogen-free facility and fed standard chow (Purina 5010) ad libitum. Enrichment and privacy were provided in mating cages by ‘breeding huts’ (Bio-Serv S3352-400). Mouse studies and all animal housing at Case Western Reserve University were approved by and performed according to the guidelines established by the Institutional Animal Care and Use Committee of CWRU.

C57BL/6J (Stock #000664), FoxP3 RFP (C57BL/6-FoxP3 tm1Flv/J, Stock #008374 mice, all on the C57BL/6 background were purchased from the Jackson Laboratory (Bar Harbor, ME). Mice were housed in a 12-h light/dark cycle specific pathogen free facility and fed standard chow (Purina 5010) ad libitum. Enrichment and privacy were provided in mating cages by “breeding huts” (Bio-Serv S3352-400). Mouse studies and all animal housing at Case Western Reserve University were approved by and performed according to the guidelines established by the Institutional Animal Care and Use Committee of CWRU.