Cd133 2 apc

Manufactured by Miltenyi Biotec

Sourced in United States

CD133/2-APC is a laboratory equipment product designed for the detection and analysis of CD133-positive cells. It is a monoclonal antibody conjugated with the fluorescent dye APC (allophycocyanin) that binds specifically to the CD133 cell surface marker.

Automatically generated - may contain errors

Lab products found in correlation

Cd133 1 apc, by Miltenyi Biotec (2 mentions) Facscalibur instrument, by BD (1 mentions) Cd105 pe, by BD (1 mentions) Cd105 fitc, by BioLegend (1 mentions) Accutase, by BD (1 mentions) Lsrii flow cytometer, by BD (1 mentions) Cd133 apc, by BD (1 mentions) Cd133 pe, by BD (1 mentions) Macs separator, by Miltenyi Biotec (1 mentions) Transcription factor buffer set, by BD (1 mentions) Ssea 1 fitc, by BD (1 mentions) Alexa fluor conjugation kits, by Thermo Fisher Scientific (1 mentions) Olig2, by R&D Systems (1 mentions) Sh800 cell sorter, by Sony (1 mentions) Dntps, by Thermo Fisher Scientific (1 mentions) Ercc spike in control, by Thermo Fisher Scientific (1 mentions) Dispase, by Thermo Fisher Scientific (1 mentions) Accumax, by Thermo Fisher Scientific (1 mentions) Cd31 apc cy7, by BioLegend (1 mentions)

Close spelling variants of this product

CD133 (122 citations) CD133-APC (32 citations) Anti-CD133/2-APC (2 citations)

4 protocols using cd133 2 apc

Quantifying CD133+ Cells in MGSC Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols

To determine the percentage of CD133+ cells of the MGSCs obtained by separation with magnetic beads, cell culture spheroids in stem enriched medium and C6 cells were stained with anti-CD133 antibody and analyzed by flow cytometry. 5x10⁵ MGSC grown as neurospheres and adherent C6 cells, neurospheres were incubated with 200 μl of CD133/2-APC Miltenyi Biotec antibody-PBS (0.1ng/ml) for 30 min in the dark. The cells were then washed with 1 ml of PBS, centrifuged at 300 x g for 5 min, and fixed with 1% paraformaldehyde. The percentage of CD133+ was determined by flow cytometry (FACSCalibur Instrument BD Biosciences), evaluating 10,000 total events. The data were analyzed using the software Cell QuestPro and Flow Jo ver. 7.6.1. (Becton Dickinson, San Jose, CA, USA).

Chavez-Cortez E.G., Vargas Felix G., Rangel López E., Sotelo J., Martínez-Canseco C., Pérez-de la Cruz V, & Pineda B. (2019). Production and Evaluation of an Avian IgY Immunotoxin against CD133+ for Treatment of Carcinogenic Stem Cells in Malignant Glioma: IgY Immunotoxin for the Treatment of Glioblastoma. Journal of Oncology, 2019, 2563092.

+ Open protocol

+ Expand

Quantification of Stem Cell Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were cultured in 6-well plates as described above. After 72 h culture, cells with approximately 80% confluence were collected with Accutase (BDSciences, San Diego, CA, USA) and analyzed for CD105 and CD133 marker expressions. Cells were stained with (i) CD105-PE (BD Pharmingen, San Diego, CA, USA, clone 266) plus CD133/1-APC (Miltenyi Biotec, Bergisch Gladbach, Germany, clone AC133); or (ii) CD105-FITC (Biolegend, San Diego, CA, USA, clone 43A3) plus CD133/2-APC (Miltenyi Biotec, Bergisch Gladbach, Germany, clone 293C3) according to the manufacturers’ protocols with the appropriate isotype controls. Subsequently, the samples were acquired in a FACSCalibur system. For each measurement, at least 10,000 cells were acquired. Data were analyzed by Flowing Software (Turku Centre for Biotechnology, Turku, Finland).

Matak D., Brodaczewska K.K., Szczylik C., Koch I., Myszczyszyn A., Lipiec M., Lewicki S., Szymanski L., Zdanowski R, & Czarnecka A.M. (2017). Functional significance of CD105-positive cells in papillary renal cell carcinoma. BMC Cancer, 17, 21.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry for Tumor Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

CD133/1-PE or CD133/2-APC (Miltenyi Biotec) and SSEA-1-FITC (BD Biosciences) antibodies were used according to manufacturer’s instructions. For TF staining in primary tumors, single cell suspensions were depleted for CD45-positive cells using a MACS separator (Miltenyi Biotec). Antibodies to SOX2 (R&D Systems), POU3F2 (Epitomics), SALL2 (Bethyl) and OLIG2 (R&D Systems) were directly conjugated to fluorophores using Alexa Fluor Conjugation Kits (Invitrogen) or DyLight conjugation kits (Pierce). The CD45-negative fraction was stained with CD133-PE or CD133-APC prior to fixation and permeabilization according to manufacturer’s protocol using the Transcription Factor Buffer Set (BD Pharmingen). Single color controls for all fluorophores were used for compensation. Flow cytometric analysis was conducted with an LSR II flow cytometer (BD Biosciences) and analysis was performed with FlowJo software (Treestar). See also supplemental methods.

Suvà M.L., Rheinbay E., Gillespie S.M., Patel A.P., Wakimoto H., Rabkin S.D., Riggi N., Chi A.S., Cahill D.P., Nahed B.V., Curry W.T., Martuza R.L., Rivera M.N., Rossetti N., Kasif S., Beik S., Kadri S., Tirosh I., Wortman I., Shalek A., Rozenblatt-Rosen O., Regev A., Louis D.N, & Bernstein B.E. (2014). Reconstructing and reprogramming the tumor propagating potential of glioblastoma stem-like cells. Cell, 157(3), 580-594.

+ Open protocol

+ Expand

Single-cell Sorting of Human Islet Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human islets were washed once in cold PBS and dissociated into single cells by enzymatic digestion with Accumax (Invitrogen), followed by digestion using freshly prepared Dispase (Fisher Scientific). Cells were filtered using a 70 μm cell strainer, quantified, and stained with LIVE/DEAD Fixable near-IR dead cell dye (Life Technologies, L10119) as a viability marker. Cells were then blocked with mouse IgG in FACS buffer (2% FBS, 10mM EGTA, in PBS), followed by staining with appropriate antibodies at 1:100 (v/v) final concentration. The following combination of antibodies was used to select endocrine cells: HPi2-Alexa-405 (Novus, NBP1-18946AF405), HPx1-Alexa-647 (Novus, NBP1-18951AF647), CD133/1-APC (Miltenyl Biotec, 130-113-668), CD133/2-APC (Miltenyl Biotec, 130-098-129), CD31-APC-Cy7 (BioLegend, 303119). Cells were then sorted using a Sony SH800 cell-sorter and a 100 μm nozzle following doublet removal. Single cells were sorted directly into 384-well plates (Bio-Rad HSP3841) containing 0.4 μL of lysis buffer with dNTPs (Invitrogen) and ERCC spike-in control (ThermoFisher). Plates were centrifuged and placed on dry ice immediately before storage at −80°C.

Camunas-Soler J., Dai X., Hang Y., Bautista A., Lyon J., Suzuki K., Kim S.K., Quake S.R, & MacDonald P.E. (2020). Patch-seq links single-cell transcriptomes to human islet dysfunction in diabetes. Cell metabolism, 31(5), 1017-1031.e4.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!