Anti p ser1981 atm

Manufactured by Abcam

Sourced in United States

Anti-p-Ser1981-ATM is a primary antibody that recognizes the phosphorylated form of the ATM (Ataxia Telangiectasia Mutated) protein at serine residue 1981. ATM is a key regulator of the DNA damage response pathway.

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Lab products found in correlation

Anti β actin, by Merck Group (3 mentions) Anti flag, by Merck Group (2 mentions) Anti puma, by Cell Signaling Technology (2 mentions) Anti cleaved poly adp ribose polymerase parp, by Cell Signaling Technology (2 mentions) Anti p ser15 p53, by Cell Signaling Technology (2 mentions) Anti ku86, by Santa Cruz Biotechnology (2 mentions) 2 14c 5 fu, by Moravek Biochemicals (2 mentions) Anti cdk2, by Santa Cruz Biotechnology (2 mentions) Anti tubulin, by Santa Cruz Biotechnology (2 mentions) Anti trf2, by Merck Group (1 mentions) Anti p53 do 1, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

P-ATM (18 citations) Anti-p-ATM (3 citations) P-ATM (Ser1981) (2 citations)

4 protocols using anti p ser1981 atm

Antibody-based Protein Analysis Protocol

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Antibodies used in this study included: anti-PUMA (Cell Signaling Technology); anti-cleaved poly ADP-ribose polymerase (PARP; Cell Signaling Technology); anti–β-actin (Sigma); anti-ATM (Abcam); anti-p-Ser1981-ATM (Abcam); anti-cleaved Caspase 7 (Stressgen Bioreagents Corp.); anti-p-Ser15-p53 (Cell Signaling Technology); anti-p53 (Oncogene Research); anti-Cdk2 (Santa Cruz Biotechnology); anti-Ku86 (Santa Cruz Biotechnology); anti-Tubulin (Santa Cruz Biotechnology); and anti-Flag (Sigma). 5-FU and MTX were purchased from Sigma. 2-¹⁴C-5-FU was purchased from Moravek Biochemicals. Cks expressing adenoviruses were described previously (13 (link)).

del Rincón S.V., Widschwendter M., Sun D., Ekholm-Reed S., Tat J., Teixeira L.K., Ellederova Z., Grolieres E., Reed S.I, & Spruck C. (2014). Cks Overexpression Enhances Chemotherapeutic Efficacy by Overriding DNA Damage Checkpoints. Oncogene, 34(15), 1961-1967.

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Antibody-based Protein Analysis Protocol

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Western Blot Analysis of DNA Damage Response

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Western blot analysis was performed as previously reported (36 (link)). Expression levels of TRF2 were evaluated by using the mouse mAb anti-TRF2 (Millipore). The DNA damage response was evaluated by using the following antibodies: rabbit mAb anti-Ser1981 p-ATM (Abcam Ltd.); rabbit pAb anti-Thr68 p-CHK2 (Cell Signaling, Beverly, MA, USA); mouse monoclonal anti-γH2AX antibody (Upstate, Lake Placid, NY, USA).
The purity of the subcellular fractions was evaluated by using the following antibodies: mouse mAb anti-EGFR (a kind gift of Dr Oreste Segatto, Regina Elena National Cancer Institute of Rome); mouse mAb anti-Lamin A/C (636, Santa Cruz Biotecnology); mouse mAb anti-GAPDH (clone 6C5, Santa Cruz Biotecnology).

Zizza P., Dinami R., Porru M., Cingolani C., Salvati E., Rizzo A., D’Angelo C., Petti E., Amoreo C.A., Mottolese M., Sperduti I., Chambery A., Russo R., Ostano P., Chiorino G., Blandino G., Sacconi A., Cherfils-Vicini J., Leonetti C., Gilson E, & Biroccio A. (2019). TRF2 positively regulates SULF2 expression increasing VEGF-A release and activity in tumor microenvironment. Nucleic Acids Research, 47(7), 3365-3382.

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Western Blot Analysis of DNA Damage Response

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Western blot and detection were performed as previously reported (36 (link)). The activation state of DNA damage response proteins was analyzed by using the following antidodies: rabbit mAb anti-Ser1981 p-ATM (Abcam Ltd.); mouse mAb anti-p53 DO-1 and rabbit pAb anti-Thr68 p-CHK2 (Cell Signaling, Beverly, MA, USA). As loading control, levels of β-ACTIN were evaluated by using the mouse mAb anti-β-actin (Sigma-Aldrich).

Zizza P., Cingolani C., Artuso S., Salvati E., Rizzo A., D'Angelo C., Porru M., Pagano B., Amato J., Randazzo A., Novellino E., Stoppacciaro A., Gilson E., Stassi G., Leonetti C, & Biroccio A. (2015). Intragenic G-quadruplex structure formed in the human CD133 and its biological and translational relevance. Nucleic Acids Research, 44(4), 1579-1590.

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