DMEM low glucose media, DMEM high glucose media, Hams’ F‐12 media, DMEM/F‐12 media (1:1), penicillin/streptomycin solution and phosphate buffered saline (PBS) were purchased from Invitrogen (Carlsbad, CA). The media additives d‐biotin, adenine hemisulfate, insulin solution, apo‐transferrin, and Nuclei EZ Prep kit were purchased from Sigma–Aldrich (St. Louis, MO). Fetal bovine serum (FBS) was obtained from HyClone (Logan, UT). Charcoal stripped FBS (CSS) was prepared within our laboratory or purchased from Invitrogen (Carlsbad, CA). Zapoglobin and Isoton II were purchased from Beckman Coulter Inc. (Fullerton, CA). Rabbit anti‐mouse IgG secondary antibody was obtained from Zymed Laboratories, Inc. Both horseradish peroxidase‐conjugated donkey anti‐rabbit and sheep anti‐mouse antibodies were purchased from GE Healthcare Biosciences (Pittsburg, PA). All tissue culture plasticware and additional chemicals were purchased from Fisher Scientific (Suwanee, GA).
Zapoglobin
Manufactured by Beckman Coulter
Sourced in United States
Zapoglobin is a reagent used in hematology analysis to determine red blood cell count and hemoglobin concentration. It serves as a lysing agent, breaking down red blood cells to facilitate accurate measurement of these parameters.
Automatically generated - may contain errors
Lab products found in correlation
Adenine hemisulfate, by Merck Group (2 mentions)
Insulin solution, by Merck Group (2 mentions)
Isoton 2, by Beckman Coulter (2 mentions)
Horseradish peroxidase conjugated donkey anti rabbit and sheep anti mouse antibodies, by GE Healthcare (2 mentions)
Apo transferrin, by Merck Group (2 mentions)
D biotin, by Merck Group (2 mentions)
Dmem low glucose media, by Thermo Fisher Scientific (2 mentions)
Ham s f12 media, by Thermo Fisher Scientific (2 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 media, by Thermo Fisher Scientific (2 mentions)
Tissue culture plasticware, by Thermo Fisher Scientific (1 mentions)
Dmem high glucose media, by Thermo Fisher Scientific (1 mentions)
Nuclei ez prep kit, by Merck Group (1 mentions)
Charcoal stripped fbs css, by Thermo Fisher Scientific (1 mentions)
Enzyme linked immunosorbent assay elisa kit, by Thermo Fisher Scientific (1 mentions)
Albumin, by Roche (1 mentions)
Methocult gf m3434 medium, by STEMCELL (1 mentions)
Hema 3 staining system, by Thermo Fisher Scientific (1 mentions)
Z1 coulter particle counter, by Beckman Coulter (1 mentions)
5 protocols using zapoglobin
1
Cell Culture Media and Reagents Procurement
2
Immunotoxicity Evaluation of COREXIT EC9500A
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COREXIT EC9500A was provided by Nalco. In this report, the use of the abbreviation, COREXIT, refers to the specific formulation of COREXIT EC9500A. Materials used for analysis of lactate dehydrogenase (LDH) and albumin were purchased from Roche Diagnostic Systems. Enzyme-linked immunosorbent assay (ELISA) kits were purchased from Life Technologies. MCh, isoproterenol (ISO), norepinephrine (NE), acetylcholine (ACh), and phenylephrine (PHEN) were purchased from Sigma-Aldrich), and Dulbecco’s modified Eagle’s medium (DMEM) with glucose was purchased from Invitrogen. All sheep red blood cells (SRBC) were drawn from a single donor animal (Lampire Biological Laboratories). IgM was measured using a commercially available kit (Life Diagnostics), Zap-oglobin for red blood cell lysis was purchased from Beckman Coulter Inc., and antibodies and reagents to identify splenocyte phenotypes were purchased from BD Pharmingen.
3
Quantifying Hematopoietic Progenitors in Irradiated Mice
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Live mice at 4, 9, 16, 25 and 40 days post IR were killed by an overdose of anaesthetic, and marrow cells were obtained from the femora (by flushing with a 22-gauge needle) and resuspended in serum-free medium. After being washed by centrifugation, cells were resuspended in α-minimum essential medium (α-MEM) with 20% foetal bovine serum. Cell number was determined after red blood cells were removed with Zapoglobin (Beckman Coulter, Miami, FL). Cells (105 per well) were cultured in MethoCult® GF M3434 medium (StemCell, Vancouver, BC, Canada) in six-well plates. MethoCult® GF M3434 medium contains interleukin-3 (IL-3), stem cell factor (SCF), interleukin-6 (IL-6) and erythropoietin (EPO) and is optimized for the detection and quantification of mouse haematopoietic progenitors such as granulocyte–macrophage progenitors and granulocyte–erythroid–macrophage–megakaryocytes (GEMMs) in bone marrow using a colony-forming cell assay. Only aggregates with more than 500 cells and a highly dense core were considered colony-forming unit (CFU)-GEMM colonies, and they were scored under a microscope 12–14 days after incubation at 37°C in a 5% CO2 humidified atmosphere. The number of CFU-GEMM colonies was determined per 105 marrow cells plated.
4
Automated WBC and Differential Counts
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Heparin-treated whole blood was used to determine the total white blood cell (WBC) counts and leukocyte differential counts (DIFF). Total WBC counts were made electronically using a Coulter Z1 particle counter (Beckman Coulter, Miami, FL, USA). Ten µL of whole blood was added to 10 mL of Isoflow® (Beckman Coulter, Miami, FL, USA) and then three drops of ZAP-OGLOBIN® (Beckman Coulter, Miami, FL, USA) were added to lyse the red blood cells, and tubes were mixed. The sample cup was placed in the counting chamber to determine the total WBC count. Whole blood smears were made, fixed in methanol, and then stained with a Hema-3 staining system (Fisher Scientific, Houston, TX, USA). Slides were viewed under a light microscope, and 100 cells per slide were visually counted to determine leukocyte differential percentages.
5
Reagents and Materials for Cell Culture
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DMEM low glucose media, DMEM/F-12 media (1:1), Hams’ F-12 media, RPMI 1640 media, penicillin/streptomycin solution, phosphate buffered saline (PBS), Pierce RIPA buffer, and the Presto Blue cell viability reagent were purchased from ThermoFisher Scientific (Waltham, MA). Metformin and the media additives d-biotin, adenine hemisulfate, insulin solution, and apo-transferrin were purchased from Sigma Aldrich (St. Louis, MO). Fetal bovine serum (FBS) was obtained from HyClone (Logan, UT). Zapoglobin and Isoton II were purchased from Beckman Coulter Inc. (Fullerton, CA). Rabbit anti-mouse IgG secondary antibody was obtained from Zymed Laboratories, Inc. Both horseradish peroxidase-conjugated donkey anti-rabbit and sheep anti-mouse antibodies were purchased from GE Healthcare Biosciences (Pittsburg, PA). All tissue culture plasticware and additional chemicals were purchased from ThermoFisher Scientific.
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