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Sulphuric acid

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Sulphuric acid is a highly corrosive, colorless, oily liquid. It is a strong mineral acid with a wide range of industrial and laboratory applications. Sulphuric acid is used in the production of fertilizers, chemicals, and pharmaceuticals. It is also utilized in various metal processing and refining operations.

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Lab products found in correlation

Hydrogen peroxide, by HiMedia (4 mentions) Potassium permanganate, by HiMedia (3 mentions) Ethanol, by HiMedia (2 mentions) 2 2 diphenyl 1 picrylhydrazyl (dpph), by HiMedia (2 mentions) Sodium hydroxide (naoh), by HiMedia (2 mentions) H2dcfda, by Thermo Fisher Scientific (2 mentions) Orthophosphoric acid, by HiMedia (1 mentions) Sodium nitrate, by Merck Group (1 mentions) Graphite flakes, by Merck Group (1 mentions) Riboflavin, by Merck Group (1 mentions) 2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Methanol, by HiMedia (1 mentions) Petroleum ether, by HiMedia (1 mentions) Crystal violet, by HiMedia (1 mentions) Diosgenin, by Merck Group (1 mentions) Hydrogen peroxide, by Merck Group (1 mentions) Potassium persulfate, by HiMedia (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Pen strep, by Thermo Fisher Scientific (1 mentions)

10 protocols using sulphuric acid

1

Synthesis of Graphene Oxide Nanoparticles

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Graphite Flakes (99%, Sigma-Aldrich), Sodium nitrate (98%), Potassium permanganate (99%), Hydrogen peroxide (30% wt), Sulphuric acid (98%), Orthophosphoric acid (35%) were all obtained from Himedia. The synthesis of Graphene Oxide nanoparticles were done according to the method described in Marcano et al. [29 (link)].
Sood K., Kaur J., Singh H., Kumar Arya S, & Khatri M. (2019). Comparative toxicity evaluation of graphene oxide (GO) and zinc oxide (ZnO) nanoparticles on Drosophila melanogaster. Toxicology Reports, 6, 768-781.
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2

Antioxidant Activity Quantification

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DPPH (2,2-diphenyl-1-picrylhydrazyl), riboflavin, and ascorbic acid were purchased from Sigma-Aldrich, USA, whereas hydrogen peroxide was obtained from HiMedia Lab Ltd., Mumbai, India. DH2O (distilled deionized water) was prepared by Camco Industries, Chandigarh (India). All other chemicals like methanol, sulphuric acid, Whatman filter paper, petroleum ether, and ethanol were purchased from HiMedia Lab Ltd., Mumbai, India.
Devi A., Dwibedi V., George N, & Khan Z.A. (2022). Response Surface Optimization for Investigating Antioxidant Potential of Camellia Sinensis and Withania Somnifera in Synergistic Manner. Proceedings of the National Academy of Sciences, India. Section B, 93(2), 397-408.
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3

Phytochemical and Bioactivity Screening

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Ethanol, sodium carbonate (99.9%), potassium persulfate (99%), sulphuric acid (95–97%), crystal violet (99.7%), and DPPH (98.9%) were obtained from Himedia (Thane (MH), India). Folin-Ciocalteu, Hydrogen peroxide (30%) was purchased from Merk (Khalapur (MH), India). L-Ascorbic acid (99%), Gallic acid (≥98%), Quercetin (≥99%), Diosgenin (≥93%), Vanillin (≥97%), 5FU (≥99%), and Cisplatin (≥98%) were obtained from Sigma (St. Louis (MO), USA) and ABTS (>98%) from Sigma (Oakville, (ON), Canada). Aluminium chloride (98%) was supplied by Finar (Ahmedabad (GJ), India). Anisaldehyde was obtained from Central drug house (CND) (Darya Ganj (ND), India). DMSO, ethyl acetate, and mEthanol were obtained from Rankem (Gurgaon (HR), India). DMEM, Pen Strep, PBS, Trypsin and FBS from Gibco (Paisley (SCT), UK), and Whatman filter paper, MTT (≥98%), H2DCFDA (≥97%), Annexin V/PI, and EnzChek® Caspase-3 Assay Kit #1were purchased from Thermofisher Scientific (Waltham (MA), USA).
Kshetrimayum V., Heisnam R., Keithellakpam O.S., Radhakrishnanand P., Akula S.J., Mukherjee P.K, & Sharma N. (2023). Paris polyphylla Sm. Induces Reactive Oxygen Species and Caspase 3-Mediated Apoptosis in Colorectal Cancer Cells In Vitro and Potentiates the Therapeutic Significance of Fluorouracil and Cisplatin. Plants, 12(7), 1446.
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4

Synthesis of Polyaniline Nanocomposites

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Aniline (C6H5NH2), sulphuric acid (H2SO4), hydrochloric acid (HCl), ammonium per sulphate ((NH4)2S2O8), potassium permanganate (KMnO4), sodium nitride (Na3N), hydrogen peroxide (H2O2) and hydrazine hydrate (H6N2O) are purchased from the Himedia. Ammonia (99.98%), acetone (99.9%), xylene (99%), benzene (99.9%), graphite and Montmorillonite (MMT) (CAS Number 1318-93-0) are obtained from Sigma Aldrich.
Singh A., Dipak P., Iqbal A., Samadhiya A., Dwivedi S.K., Tiwari D.C., Tiwari R.K, & Pandey K.N. (2023). Fast response and recovery polyaniline montmorillonite reduce graphene oxide polymer nanocomposite material for detection of hydrogen cyanide gas. Scientific Reports, 13, 8074.
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5

Antioxidant Activity Determination

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Sulphuric acid, sodium hydroxide, aluminium chloride, Folin-Ciocalteu’s (FC) reagent, gallic acid, DPPH (2,2-diphenyl-1-picrylhydrazyl), TPTZ (2,4,6-tri(2-pyridyl)-s-triazine), ABTS (2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid), and other chemicals were procured from Himedia Laboratories (Mumbai, India).
Sagar N.A, & Pareek S. (2020). Dough rheology, antioxidants, textural, physicochemical characteristics, and sensory quality of pizza base enriched with onion (Allium cepa L.) skin powder. Scientific Reports, 10, 18669.
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6

Topical Wound Treatment Formulations

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Drugs, chemicals, and reagents that were used for the study include nitrofurazone skin ointment United States Pharmacopeia (USP)0.2% (Shanghai General Pharmaceuticals Co., Ltd., China), silver sulfadiazine skin ointment USP 1% (Galentic Pharma Pvt. Ltd., India), ketamine hydrochloride 50 mg/mL injection USP (Neon Laboratories Limited, India), diazepam 5mg/mL injection (Mission Pharma, Denmark), hard paraffin (Lab Tech Chemicals), wool fat (Uni-Chem Chemical Reagents), cetostearyl alcohol (Blulux Laboratories, India), methanol (99.9%) (Alpha Chemika, India), ethanol (70%) (Harego Chemicals, Addis Ababa), Ethyl acetate (Nice Chemicals Pvt. Ltd., India), chloroform (BDH Chemicals Ltd., England), bees wax (Bo International, Delhi, India),10% buffered formalin (Sigma Diagnostics™, Livonia), paraffin wax (Reliance Wax Co., Ltd. China), hematoxylin and eosin (Alpha Chemika Maharashtra, India), hydrochloric acid (Blulux Laboratories Pvt. Ltd., India), Sodium hydroxide (Blulux Laboratories Pvt. Ltd.,India), sulphuric acid (HiMedia Laboratories Pvt. Ltd., India), lead acetate (Guangdong Guanghua chemicals factories, China), ferric chloride (Blulux Laboratories Pvt. Ltd., India) and Wagner’s reagent (Research-Lab, Fine Chem Industries, India).
Lambebo M.K., Kifle Z.D., Gurji T.B, & Yesuf J.S. (2021). Evaluation of Wound Healing Activity of Methanolic Crude Extract and Solvent Fractions of the Leaves of Vernonia auriculifera Hiern (Asteraceae) in Mice. Journal of Experimental Pharmacology, 13, 677-692.
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7

Phytochemical Screening of Medicinal Ingredients

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Distilled water (Ethiopian Pharmaceutical Manufacturing, Ethiopia), loperamide HCl (Bafna Pharmaceuticals, Ltd., India), castor oil (Shiba Pharmaceuticals and Chemicals, Yemen), methanol (Lab Chemicals, Avishkar, India), activated charcoal (Okhla Industrial Area, India), and atropine sulphate inj. (0.1%) (Jeil Pharm. Co. Ltd., Korea) were used. Other reagents, such as glacial acetic acid (Blulux Laboratories (P) Ltd., India), ammonia (Loba Chemie Pvt. Ltd., India), ferric chloride (Blulux Laboratories (P) Ltd., India), Mayer's reagent (Research-Lab Fine Chem Industries, India), hydrochloric acid (Blulux Laboratories (P) Ltd., India), chloroform (Nice Chemicals Pvt. Ltd., India), sulphuric acid (HiMedia Laboratories Pvt. Ltd., India), and benzene (Blulux Laboratories (P) Ltd., India), were used in the phytochemical screening test.
Mekonnen B., Asrie A.B, & Wubneh Z.B. (2018). Antidiarrheal Activity of 80% Methanolic Leaf Extract of Justicia schimperiana. Evidence-based Complementary and Alternative Medicine : eCAM, 2018, 3037120.
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8

Phytochemical Analysis of Castor Oil

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Absolute methanol (Carlo Erba reagents, S.A.S, France), castor oil (Amman Pharmaceutical Industries, Jordan), activated charcoal (Acuro Organics Ltd, New Delhi, India), loperamide (Daehwa Pharmaceutical, Republic of Korea), distilled water (Ethiopian Pharmaceutical Manufacturing Factory, Epharm, Ethiopia), Wagner's reagent (Research-Lab Fine Chem Industries, India), lead acetate 1% (Guangdong Guanghua chemicals factories, China), sodium hydroxide (Blulux Laboratories (P) Ltd., India), ferric chloride (Blulux Laboratories (P) Ltd., India), chloroform (Hi-Media Laboratory Reagents, India), sulphuric acid (HiMedia Laboratories Pvt. Ltd., India), and lyophilizer (Lab freeze Instruments Group Co., Ltd., Japan) were used. All experimental supplies were analytical grade and purchased from Gondar Pharmaceutical Fund Supply Agency and nearby suppliers.
Ferede Y.A., Zewdu W.S., Zeleke M.M, & Alemu M.A. (2021). Evaluation of Antidiarrheal Activity of 80% Methanolic Extract of the Leaves of Cordia africana (Lamiaceae) in Mice. Evidence-based Complementary and Alternative Medicine : eCAM, 2021, 3627878.
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9

Graphite-Quercetin Composite Synthesis

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Expanded graphite powder (average particle size ∼100 μm) and quercetin (QSR) were purchased from Sigma-Aldrich. Polyvinylpyrrolidone (PVP, Mw ∼ 40 000) was obtained from Calbiochem. Hydrogen peroxide (H2O2, 30 wt% aqueous solution), sodium nitrate (NaNO3), sulphuric acid (H2SO4), potassium permanganate (KMnO4), iron(ii) sulfate heptahydrate (FeSO4·7H2O), iron(iii) chloride hexahydrate (FeCl3·6H2O), sodium hydroxide (NaOH), fetal bovine serum (FBS), penicillin–streptomycin, sulforhodamine B (SRB) assay and Eagle's minimum essential medium (MEM) with NEAA were obtained from Himedia. N,N′-dicyclohexyl carbodiimide (DCC) and 4-dimethylaminopyridine (DMAP) were obtained from Sisco Research Laboratories Pvt. Ltd (SRL).
Matiyani M., Rana A., Pal M., Rana S., Melkani A.B, & Sahoo N.G. (2022). Polymer grafted magnetic graphene oxide as a potential nanocarrier for pH-responsive delivery of sparingly soluble quercetin against breast cancer cells. RSC Advances, 12(5), 2574-2588.
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10

Formulation and Evaluation of Topical Wound Dressings

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Wool fat (Uni-Chem chemical reagents, Serbia), hard paraffin (Lab tech chemicals, Ethiopia), white soft paraffin (Ethiopian pharmaceuticals manufacturing, SH.CO, Ethiopia), cecostearyl alcohol (Blulux Laboratories (P) Ltd., India), methanol absolute (AppliChem, Germany), Chloroform (Nice Chemicals Pvt. Ltd., India), ethyl acetate (Carlo Erba reagent S.A.S., France), 0.2% nitrofurazone skin ointment (shanghai general pharmaceutical CO., LTD, China), 1% silver sulfadiazine cream (Galentic pharm PVT. LTD, India), hydrochloric acid (Blulux Laboratories (P) Ltd., India), ethanol absolute (Blulux Laboratories (P) Ltd., India), ferric chloride (Blulux Laboratories (P) Ltd., India), ketamine hydrochloride injection (Neon laboratories limited, India), diazepam injection (Gland pharm limited, India), normal saline 0.9% (IV infusion BP Medsol pharmaceuticals), sodium hydroxide (Blulux Laboratories (P) Ltd., India), Wagner’s reagent (Research-Lab Fine Chem Industries, India), lead acetate (Guangdong Guanghua chemicals factories, China), sulphuric acid (HiMedia Laboratories Pvt. Ltd., India), dichloromethane (Blulux Laboratories (P) Ltd., India), halothane inhalation (Piramal enterprises limited, India), acetic anhydride, distilled water and bee wax were used. All the chemicals and reagents were analytical grade.
G/giorgis S.G., Ambikar D., Tsegaw A, & Belayneh Y.M. (2022). Wound Healing Activity of 80% Methanolic Crude Extract and Solvent Fractions of the Leaves of Justicia schimperiana (Hochst. ex Nees) T. Anderson (Acanthaceae) in Mice. Journal of Experimental Pharmacology, 14, 167-183.
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