Log In Sign up
The largest database of trusted experimental protocols

Elisa quantitation kit

Manufactured by Fortis Life Sciences
Visit Supplier
Sourced in United States

The ELISA quantitation kit is a laboratory tool used to measure the concentration of specific proteins or other analytes in a sample. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify the target substance. The kit provides the necessary reagents and protocols to perform this analysis.

Automatically generated - may contain errors

Lab products found in correlation

Quantichrom urea assay kit, by BioAssay Systems (3 mentions) Cobas 8000, by Roche (2 mentions) Chicken type 2 collagen, by Merck Group (1 mentions) Low endotoxin bsa a 9430, by Merck Group (1 mentions) Cellometer auto t4 bright field cell counter, by Revvity (1 mentions) Rifampin, by Merck Group (1 mentions) Urea nitrogen assay kit, by Sangon (1 mentions) Quant it picogreen dsdna assay kit, by Thermo Fisher Scientific (1 mentions) Creatinine assay kit, by BioAssay Systems (1 mentions) Tmb substrate, by Fortis Life Sciences (1 mentions) Dtx 880 multimode detector, by Beckman Coulter (1 mentions) Facscalibur, by BD (1 mentions) Cytometric bead array system, by BD (1 mentions) Genios pro, by Tecan (1 mentions) N acetylgalactosamine, by Merck Group (1 mentions) Kc4 version 3, by Agilent Technologies (1 mentions) 3 3 5 5 tetramethylbenzidine (tmb), by Merck Group (1 mentions)

31 protocols using elisa quantitation kit

1

Quantifying Collagen-Specific IgG

Check if the same lab product or an alternative is used in the 5 most similar protocols
The serum concentrations of IgG were measured with an enzyme-linked immunosorbent assay (ELISA) quantitation kit (Bethyl Laboratories). For the assessment of collagen-specific IgG, plates were coated with 10 μg ml−1 chicken collagen type II (Sigma) instead of the capture antibody.
Harre U., Lang S.C., Pfeifle R., Rombouts Y., Frühbeißer S., Amara K., Bang H., Lux A., Koeleman C.A., Baum W., Dietel K., Gröhn F., Malmström V., Klareskog L., Krönke G., Kocijan R., Nimmerjahn F., Toes R.E., Herrmann M., Scherer H.U, & Schett G. (2015). Glycosylation of immunoglobulin G determines osteoclast differentiation and bone loss. Nature Communications, 6, 6651.
+ Open protocol
+ Expand
2

Quantifying Secreted and Intracellular Ig Light Chains

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ig light chain ELISA was performed as described previously [3 (link)] and quantified using the lambda (λ) or kappa (κ) ELISA Quantitation Kit (Bethyl Laboratories) according to manufacturer’s instructions. In-vitro secreted light chain was measured from conditioned media (1:100 dilution) collected from 3x106 cells following 24 hour culture. Intracellular light chain was measured from 500ng cell lysate protein collected. Plasma samples from mice bearing xenograft tumors and age-matched non-tumor bearing mice (as controls) were tested at 1:100 dilution. For HSF1 knockdown studies, cells were plated 72 hours post-transduction.
Fok J.H., Hedayat S., Zhang L., Aronson L.I., Mirabella F., Pawlyn C., Bright M.D., Wardell C.P., Keats J.J., De Billy E., Rye C.S., Chessum N.E., Jones K., Morgan G.J., Eccles S.A., Workman P, & Davies F.E. (2018). HSF1: Essential for Myeloma Cell Survival and A Promising Therapeutic Target. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(10), 2395-2407.
+ Open protocol
+ Expand
3

Murine Protein-Overload Renal Injury Model

Check if the same lab product or an alternative is used in the 5 most similar protocols
This study was approved by the Committee on the Use of Live Animals in Teaching and Research of The University of Hong Kong and was performed in accordance with the National Institute of Health Guide for Care and Use of Laboratory Animals. Murine protein-overload model was established in C57BL6 mice at 6 wks as previously described [23] (link), [24] (link). Uninephrectomy was performed under anesthesia 5 days before BSA injections began. Low endotoxin BSA A-9430 (Sigma) was given 5 days a week intraperitoneally at 10 mg/g body weight for 4 wks. Control mice received the same volume of saline. Beginning on day 7 of BSA injection, mouse BM-MSCs (1×106 cells/mouse) were injected intravenously into uninephrectomized mice with or without BSA treatment at weekly intervals until sacrifice at wk 4 of BSA injection. Renal CCL-2, CCL-5, α-SMA, collagen IV mRNA and protein expression were evaluated in 4 mice randomly selected from each group. Urinary albumin was measured by ELISA quantitation kit (Bethyl Laboratories, Montgomery, AL, USA), and blood urea nitrogen (BUN), urine and serum creatinine were determined by enzymatic method (Stanbio Laboratory, Boerne, TX, USA).
Wu H.J., Yiu W.H., Li R.X., Wong D.W., Leung J.C., Chan L.Y., Zhang Y., Lian Q., Lin M., Tse H.F., Lai K.N, & Tang S.C. (2014). Mesenchymal Stem Cells Modulate Albumin-Induced Renal Tubular Inflammation and Fibrosis. PLoS ONE, 9(3), e90883.
+ Open protocol
+ Expand
4

Quantifying Hepatocyte Function

Check if the same lab product or an alternative is used in the 5 most similar protocols
A human albumin enzyme-linked immunosorbent assay (ELISA) quantitation kit (Bethyl Laboratories, Inc., Montgomery, TX, USA) and QuantiChrom™ urea assay kit (BioAssay Systems, Hayward, CA, USA) were used to quantitate free albumin and urea levels, respectively, as measures of hepatocyte function. Albumin secretion and urea synthesis of hepatocytes in mono- and co-culture were normalised to one million seeded viable hepatocytes.
Qin H.H., Filippi C., Sun S., Lehec S., Dhawan A, & Hughes R.D. (2015). Hypoxic preconditioning potentiates the trophic effects of mesenchymal stem cells on co-cultured human primary hepatocytes. Stem Cell Research & Therapy, 6, 237.
+ Open protocol
+ Expand
5

Cytokine Quantification in Wound Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
TNF-α, interleukin (IL)-1β, and IL-6 levels were determined in the wound tissue (in a portion of would tissue from another set of random animals [n = 6]) using rat enzyme-linked immunosorbent assay (ELISA) quantitation kit (Bethyl Laboratories Inc., Montgomery, TX, United States of America).
Yan Z., Li S, & Gong Z. (2023). Bisacurone gel ameliorated burn wounds in experimental rats via its anti-inflammatory, antioxidant, and angiogenic properties. Acta Cirúrgica Brasileira, 38, e382423.
+ Open protocol
+ Expand
6

Quantification of Albumin Secretion

Check if the same lab product or an alternative is used in the 5 most similar protocols
At the endpoint of the differentiation process, the supernatant of cultured cells was collected. Albumin secretion in the supernatant was measured with a human albumin enzyme-linked immunosorbent assay (ELISA) quantitation kit (Bethyl, Montgomery, TX, USA) according to the manufacturer’s instructions. Cells were trypsinized and counted with Cellometer Auto T4 Bright Field Cell Counter (Nexcelom Bioscience, Lawrence, MA, USA). The albumin secretion was normalized to total cell numbers.
Du C., Feng Y., Qiu D., Xu Y., Pang M., Cai N., Xiang A.P, & Zhang Q. (2018). Highly efficient and expedited hepatic differentiation from human pluripotent stem cells by pure small-molecule cocktails. Stem Cell Research & Therapy, 9, 58.
+ Open protocol
+ Expand
7

Analyzing Cecal Microbiome and Metabolites

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Bacterial genomic DNA was extracted from cecal digesta using an Isofecal DNA extraction kit (Nippon Gene, Co., Ltd., Tokyo, Japan) according to the manufacturer's instructions. The cecal microflora was analyzed using a terminal restriction fragment length polymorphism method as described previously (18 (link)). Cecal organic acids were measured as described previously (19 (link)). Fecal acidic sterols were analyzed using an internal standard (nor-deoxycholic acid; Steraloids, Wilton, NY, USA) by gas chromatography as described previously. The total IgA concentration in feces was measured using an ELISA quantitation kit (Bethyl Laboratories Inc., Montgomery, TX, USA). Mucins were extracted according to the method of Bovee-Oudenhoven et al (20 (link)) and quantitated using a fluorometric assay (21 (link)). The activities of harmful fecal enzymes, such as tryptophanase, β-glucuronidase and β-glucosidase, were determined as described previously (22 (link)).
YANG Y., SITANGGANG N.V., OKAZAKI Y., TOMOTAKE H., ARITA K., ASHIDA T, & KATO N. (2015). Supplemental fermented plant product (‘Manda Koso’) reduces succinate and deoxycholate, as well as elevates IgA and mucin levels, in rats fed a high-fat diet. Biomedical Reports, 3(6), 787-791.
+ Open protocol
+ Expand
8

Quantification of Hepatocyte Secretion and Enzyme Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Albumin was measured in collected cell culture supernatant using a commercial human albumin-specific enzyme-linked immunosorbent assay (ELISA) quantitation kit (Bethyl Laboratories). Urea was measured in collected cell culture supernatant using a commercial diacetylmonoxime-based blood urea nitrogen diagnostic kit (StanBio Laboratory). Relative CYP3A4 and CYP2C9 activity was determined using commercial live-cell luciferin-based luminescence assay kits (Promega Corporation). For CYP induction studies, rifampin (Sigma-Aldrich) was diluted to a concentration of 25 µM in H–E medium and devices were stimulated for 72 h, with media being replenished every 24 h. All assays were performed per manufacturer instructions and absorbance/luminescence measurements were done using a Tecan plate reader.
Chhabra A., Song H.H., Grzelak K.A., Polacheck W.J., Fleming H.E., Chen C.S, & Bhatia S.N. (2022). A vascularized model of the human liver mimics regenerative responses. Proceedings of the National Academy of Sciences of the United States of America, 119(28), e2115867119.
+ Open protocol
+ Expand
9

Quantifying Liver Function Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
The concentrations of albumin (ALB), urea nitrogen and the total bile acids in culture medium were measured by Human Albumin Enzyme Linked Immunosorbent (ELISA) Quantitation Kit (BETHYL), Urea Nitrogen Assay Kit (Sangon Biotech, Shanghai, China) and Total Bile Acid Kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according the manufacturer’s instructions.
Wang J., Situ P., Chen S., Wu H., Zhang X., Liu S., Wang Y., Xie J., Chen H, & Duan Y. (2022). Hepatic Polarized Differentiation Promoted the Maturity and Liver Function of Human Embryonic Stem Cell-Derived Hepatocytes via Activating Hippo and AMPK Signaling Pathways. Cells, 11(24), 4117.
+ Open protocol
+ Expand
10

Quantification of Albumin and Urea

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human albumin enzyme-linked immunosorbent assay (ELISA) quantitation kit (Bethyl Laboratories, Inc., Montgomery, TX, USA) and Direct Urea Nitrogen Color Reagent and Direct Urea Nitrogen Acid Reagent (Standbio Laboratory, Boerne, TX, USA) were used to measure albumin and urea respectively according to manufacturer’s instructions and as described in32 (link). Functional data was normalized to cell numbers quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies).
Tasnim F., Singh N.H., Tan E.K., Xing J., Li H., Hissette S., Manesh S., Fulwood J., Gupta K., Ng C.W., Xu S., Hill J, & Yu H. (2020). Tethered primary hepatocyte spheroids on polystyrene multi-well plates for high-throughput drug safety testing. Scientific Reports, 10, 4768.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!