C011 2 1

Manufactured by Nanjing Jiancheng

Sourced in China

The C011-2-1 is a laboratory equipment product from Nanjing Jiancheng. It is a device designed for conducting scientific experiments and measurements. The core function of this product is to provide a controlled environment for various laboratory procedures.

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Lab products found in correlation

35 protocols using c011 2 1

Metabolic Cage Assessment of Renal Function

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A metabolic cage was used to collect urine. Urinary volume, urinary glucose excretion (BC2505; Solarbio), urinary albumin (MM-44287M1; MEIMIAN), and urinary creatinine (C011-2-1; Nanjing JianCheng Bioengineering Institute) were measured every 2 weeks. At the end of the 12-week intervention, blood urea nitrogen (BUN) (BC1535; Solarbio) and creatinine (C011-2-1; Nanjing JianCheng Bioengineering Institute) were determined by biochemical methods, and the kidneys were weighed.

Zhu Q., Zhou Q., Luo X.L., Zhang X.J, & Li S.Y. (2023). Combination of canagliflozin and puerarin alleviates the lipotoxicity to diabetic kidney in mice. The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology, 27(3), 221-230.

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Biochemical Markers of Renal Function

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Serum creatinine and blood urea nitrogen were measured by commercial kits (C013-1-1 and C011-2-1, Nanjing Jiancheng, China). Relative LDH release was determined using a commercial kit LDH (BC0680, Solarbio, Beijing, China). The levels of renal malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were measured using specific kits (A003-1, A001-1, A007-1, A061-1, Nanjing Jiancheng, China). Data were normalized to total protein concentration determined by the BCA protein assay kit (P0012S, Beyotime, China).

Zhao Y., Ding Z., Ge W., Liu J., Xu X., Cheng R, & Zhang J. (2021). Riclinoctaose Attenuates Renal Ischemia-Reperfusion Injury by the Regulation of Macrophage Polarization. Frontiers in Pharmacology, 12, 745425.

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Biomarker Evaluation of Kidney Function

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Levels of blood urea nitrogen (BUN), serum creatinine (SCr), and 24-h urine protein (24 h-UP) were determined using assay kits (C013-2-1, C011-2-1, C035-2-1, Nanjing Jiancheng Bioengineering Institute, China), according to the manufacturer’s protocols.

Wang J., Wang Y., Liu Y., Cai X., Huang X., Fu W., Wang L., Qiu L., Li J, & Sun L. (2022). Ferroptosis, a new target for treatment of renal injury and fibrosis in a 5/6 nephrectomy-induced CKD rat model. Cell Death Discovery, 8, 127.

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Nanoparticle Toxicity Screening in Mice

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Healthy mice were intravenously injected with different nanoparticles for 24 h or 2 weeks and the blood was collected for detecting CRE, ALT and AST concentrations according to the kit instructions (Cat No. C011-2-1, C010-2-1, C009-2-1, Jiancheng, Nanjing, China). The livers were collected and stained with H&E for histopathological analysis.

Zhuo X., Wu Y., Fu X., Li J., Xiang Y., Liang X., Mao C, & Jiang Y. (2023). Genome editing of PAR2 through targeted delivery of CRISPR-Cas9 system for alleviating acute lung inflammation via ERK/NLRP3/IL-1β and NO/iNOS signalling. Acta Pharmaceutica Sinica. B, 14(3), 1441-1456.

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Serum Biomarkers in Mice

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The serum levels of blood urea nitrogen (BUN) and serum creatinine (Scr) in mice were measured by urease and sarcosine oxidase assays, respectively, according to the manufacturer (Jiancheng Bioengineering Institute, Nanjing, China; kit numbers C013-2-1 and C011-2-1, respectively).

Gong Q., Lai T., Liang L., Jiang Y, & Liu F. (2023). Targeted inhibition of CX3CL1 limits podocytes ferroptosis to ameliorate cisplatin-induced acute kidney injury. Molecular Medicine, 29, 140.

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Evaluating Organ Damage in Ischemia-Reperfusion

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Lung,⁵² (link) liver,⁵³ (link) and kidney⁵⁴ (link) histopathological damage scores were assessed 6 h after reperfusion as described previously. The lung wet/dry (W/D) weight ratio was measured as previously described.⁵⁵ (link) An alanine aminotransferase (ALT) assay kit (C009-2-1, Nanjing Jiancheng Bioengineering Institute) and aspartate aminotransferase assay kit (C010-2-1, Nanjing Jiancheng Bioengineering Institute) were used to detect ALT and AST levels in plasma to reflect liver damage. A creatinine (Cr) assay kit (C011-2-1, Nanjing Jiancheng Bioengineering Institute) was used to detect Cr levels in plasma to reflect kidney damage. The specific steps of all kits are shown in the kit instructions.

Deng F., Hu J.J., Lin Z.B., Sun Q.S., Min Y., Zhao B.C., Huang Z.B., Zhang W.J., Huang W.K., Liu W.F., Li C, & Liu K.X. (2023). Gut microbe-derived milnacipran enhances tolerance to gut ischemia/reperfusion injury. Cell Reports Medicine, 4(3), 100979.

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Comprehensive Blood Biomarker Analysis

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Fasting venous blood samples were collected with standard procedure by trained investigators to measure the concentrations of Na, K, and Cl, and testosterone, cortisol, creatinine, and copeptin.
The concentrations of electrolyte (including the sodium, potassium, chloride, calcium, magnesium, and phosphate) were tested, as described before in our previous study [14 (link)]. The testosterone, cortisol, and copeptin levels were determined by a trained investigator using Imark microplate reader (Bio-Rad 680, Bio-Rad, Hercules, CA, USA). The creatinine level was assessed using the sarcosine oxidase method (C011-2-1, Jiancheng, Nanjing, China).

Zhang J., Zhang N., Li Y., He H, & Ma G. (2022). Habitual Total Drinking Fluid Intake Did Not Affect Plasma Hydration Biomarkers among Young Male Athletes in Beijing, China: A Cross-Sectional Study. Nutrients, 14(11), 2311.

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Comprehensive Nutrient Analysis Across Samples

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Crude protein in the feed, feathers, carcasses, chyme, and feces was determined with a Kjeldahl nitrogen meter [27 (link)]. Tryptophan was determined by the national standard GBT15400-2018 method; other amino acids in the feed, feathers, carcasses, digesta, and feces were determined by a fully automated amino acid analyzer [28 (link)]. Creatinine content in the feces from trial 2 was analyzed with a Sarcosine oxidase method using its specific assay kits (C011-2-1) purchased from the Nanjing Jiancheng Bioengineering Institute of China. TiO₂ in feed and ileal digesta from trial 4 was measured by the spectrophotometric method [29 ].

Liu M., Xia Z.Y., Li H.L., Huang Y.X., Refaie A., Deng Z.C, & Sun L.H. (2024). Estimation of Protein and Amino Acid Requirements in Layer Chicks Depending on Dynamic Model. Animals : an Open Access Journal from MDPI, 14(5), 764.

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Biochemical Profiling of Serum Markers

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Serum levels of creatinine (Cr), blood urea nitrogen (BUN), calcium (Ca), and phosphorus (P) were determined by spectrophotometry (Multiskan GO. Thermo Scientific) using commercial kits (Cat no: C011-2-1, C013-2-1, C004-2-1, and C006-1-1, Nanjing Jiancheng Bioengineering, Nanjing, China) according to the manufacturer’s instructions. Quantification of serum parathyroid hormone (PTH) and fibroblast growth factor 23 (FGF23) was performed using commercial enzyme-linked immunosorbent assay kits (ELISA Cat no: E-EL-M0709c and E-EL-M2415c, Elabscience, Wuhan, China) according to the manufacturer’s instructions.

Yang X., Liu Y., Zhu X., Chen P., Xie X., Xu T., Zhang X, & Zhao Y. (2023). Vascular, valvular and kidney calcification manifested in mouse models of adenine-induced chronic kidney disease. Renal Failure, 45(1), 2228920.

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Kidney Function and Oxidative Stress

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Serum was collected to measure BUN (C013-2-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) and Scr (C011-2-1, Nanjing Jiancheng) by colorimetric. The kidney tissue was collected and homogenized (10%, w/v), and the lysate was collected to measure MDA (S0131S, Beyotime, Shanghai, China) content, SOD (S0109, Beyotime), and CAT (S0051, Beyotime) activities using commercially available kits.

Pan J., Zhu J., Li L., Zhang T, & Xu Z. (2023). Salidroside attenuates LPS-induced kidney injury through activation of SIRT1/Nrf2 pathway. Human & experimental toxicology, 42.

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