Human map kinase phosphorylation antibody array kit

Human MAP Kinase Phosphorylation Antibody Array Kit (Abcam, Cambridge, UK) was used to elucidate the changes in signal transduction pathways in DPCs. A total of 17 human proteins for phosphorylation were analyzed. Cells were treated with 1.5 and 3 mM of adenosine for an appropriate time and then collected for protein phosphorylation analysis. Cells treated with the vehicle medium were used as the non-treated control. The conventional immunoblot process was performed following the manufacturer’s protocol. The resulting blots were analyzed under the same condition using iBright FL1000 (Invitrogen, Waltham, MA, USA).

Human growth factor antibody array kit (Abcam, Cambridge, UK) and human MAP kinase phosphorylation antibody array kit (Abcam, Cambridge, UK) were used to elucidate the changes in growth factor profiles and signal transduction pathways in cultured human dermal fibroblasts. A total of 41 human growth factors and 17 human MAPK phosphorylation were analyzed. Briefly, cells were treated with 1.5, 3 mM of adenosine and 50, 100 µM of cordycepin for appropriate time and then collected for growth factor and MAPK phosphorylation analysis. Cells treated with vehicle medium were used as non-treated control. Conventional immunoblot process was performed following the manufacturer’s protocol. The resulting blots were analyzed under identical condition using iBright FL1000 (Invitrogen, Waltham, MA, USA).