Silencer sirna transfection 2 kit

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom

The Silencer® siRNA Transfection II Kit is a laboratory product designed for the efficient delivery of small interfering RNA (siRNA) into mammalian cells to facilitate gene silencing experiments. The kit includes optimized transfection reagents and protocols to enable effective siRNA uptake and target gene knockdown.

Automatically generated - may contain errors

Lab products found in correlation

Silencer select pre designed small interfering rna sirna, by Thermo Fisher Scientific (1 mentions) Siport neofx transfection reagent, by Thermo Fisher Scientific (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Opti mem 1 medium, by Thermo Fisher Scientific (1 mentions) Hiperfect transfection reagent, by Qiagen (1 mentions)

Close spelling variants of this product

Silencer siRNA Transfection Kit Silencer siRNA transfection SiRNA Transfection Kit Silencer siRNA SiRNAs

10 protocols using silencer sirna transfection 2 kit

siRNA Silencing of DNMT1 and HDAC1 in MDA-MB-231 Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Validated siRNA for DNMT1 (ID: S4215) and HDAC1 (ID: S73) and the positive/negative control RNAi (Thermo Scientific, Applied Biosystems) were transfected into ERα-negative breast cancer MDA-MB-231 cells by the indicated treatment using the Silencer siRNA Transfection II Kit (Thermo Scientific) according to the protocols provided by the manufacturer^{24 (link)}. Relevant gene expression and enzymatic activities were detected ~72 h after transfection.

Li Y., Meeran S.M, & Tollefsbol T.O. (2017). Combinatorial bioactive botanicals re-sensitize tamoxifen treatment in ER-negative breast cancer via epigenetic reactivation of ERα expression. Scientific Reports, 7, 9345.

+ Open protocol

+ Expand

Silencing DNMT1 and HDAC1 in SHR cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Validated siRNA for DNMT1 (ID: S4215) and HDAC1 (ID: S73) and the positive/negative control RNAi (Thermo Scientific, Applied Biosystems) were transfected into early transformed SHR cells by indicated treatment using the Silencer siRNA Transfection II Kit (Thermo Scientific) according to the protocols provided by the manufacturer [23 (link)].

Li Y., Buckhaults P., Cui X, & Tollefsbol T.O. (2016). Combinatorial epigenetic mechanisms and efficacy of early breast cancer inhibition by nutritive botanicals. Epigenomics, 8(8), 1019-1037.

+ Open protocol

+ Expand

Knockdown of SREBP2 in Gastric Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Human Sterol Regulatory Element‐Binding Protein 2 (SREBP2) small interfering RNA (siRNA) was acquired from Genechem, a company based in Shanghai, China. The Silencer™ siRNA Transfection II Kit (Thermo, AM1631) was used to transfect MGC803 or AGS cells to achieve knockdown of the target gene. Cells that underwent transfection were used for further tests after 48 h. In this study, we assessed the transfection efficiency of GC cells using quantitative polymerase chain reaction (qPCR).

Zhu L, & Wang H. (2024). Cholesterol‐regulated cellular stiffness may enhance evasion of NK cell‐mediated cytotoxicity in gastric cancer stem cells. FEBS Open Bio, 14(5), 855-866.

+ Open protocol

+ Expand

Gene Silencing in Periodontal Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

One d prior to the transfection, 5 × 10⁶ of GEC or EC was seeded in a six-well plate. Transfection with 5 nM of Silencer™ Select Pre-Designed small interfering RNA (siRNA) targeted against MAL (ID: s195607, Ref: 4392420, Thermo Fisher), TRAM (ID: s51477, Ref: 4392420, Thermo Fisher), or with a negative control was performed using Lipofectamine 3000 and the Silencer™ siRNA Transfection II Kit (Thermo Fisher) according to the manufacturer’s protocol. Transfected cells were then infected with P. gingivalis according to the experimental design.

Bugueno I.M., Benkirane-Jessel N, & Huck O. (2021). Implication of Toll/IL-1 receptor domain containing adapters in Porphyromonas gingivalis-induced inflammation. Innate Immunity, 27(4), 324-342.

+ Open protocol

+ Expand

Nrf2 Downregulation in BV2 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The pre-designed Small interfering RNA (siRNA) directed against Nrf2 (Ambion, Austin, TX; Genbank accession nos. NM_010902.4) was used to down-regulate Nrf2, and a commercial negative control (NC) sequence (Ambion, Cat# 4390843) was used to monitor the off-target effect. Transfections were accomplished using the Silencer™ siRNA Transfection II Kit according to the supplier's instructions (Ambion, Cat# AM1631). The medium was replaced with fresh DMEM medium 24 h after transfection. Then BV2 cells were pretreated with DBZ, followed by treatment with LPS (1 μg·mL⁻¹) for indicated time periods.

Liao S., Wu J., Liu R., Wang S., Luo J., Yang Y., Qin Y., Li T., Zheng X., Song J., Zhao X., Xiao C., Zhang Y., Bian L., Jia P., Bai Y, & Zheng X. (2020). A novel compound DBZ ameliorates neuroinflammation in LPS-stimulated microglia and ischemic stroke rats: Role of Akt(Ser473)/GSK3β(Ser9)-mediated Nrf2 activation. Redox Biology, 36, 101644.

+ Open protocol

+ Expand

Knockdown of EEA1 in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human EEA1 siRNA (a pool of 3 target-specific 20–25 nt siRNAs) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Cells were transfected with control siRNA or EEA1 siRNA-1 at a final concentration of 40 nM for 48 h using siPORT NeoFX transfection reagent from the Silencer siRNA Transfection II Kit (Ambion, Applied Biosystems, Foster City, CA) in OptiMEM I medium with GlutaMAX (Invitrogen, Carlsbad, CA).

András I.E, & Toborek M. (2014). HIV-1 STIMULATES NUCLEAR ENTRY OF AMYLOID BETA VIA DYNAMIN DEPENDENT EEA1 AND TGF-β/SMAD SIGNALING. Experimental cell research, 323(1), 66-76.

+ Open protocol

+ Expand

Mouse Macrophage siRNA Transfection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse peritoneal macrophages were transfected with specific siRNA using Silencer® siRNA Transfection II Kit (Ambion) according to the manufacturer's instructions. The cells were then recovered for 48 h before stimulation. The siRNA oligonucleotide were designed and synthesized by Shanghai RIBOBIO Co., LTD (Guangzhou, China). Sequence of MyD88-specific siRNA1 and ‘Nonsense’ siRNA were shown in Supplementary table 1.

Yan Q., Sun L., Zhu Z., Wang L., Li S, & Ye R.D. (2014). Jmjd3-mediated epigenetic regulation of inflammatory cytokine gene expression in serum amyloid A-stimulated macrophages. Cellular signalling, 26(9), 1783-1791.

+ Open protocol

+ Expand

Knockdown of IRF7 in THP-1 cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human monocytic THP-1 cells were transfected with specific siRNA using Silencer^® siRNA Transfection II Kit (Ambion) according to the manufacturer’s instructions. The cells were then recovered for 48 hours before stimulation. The siRNA oligonucleotides were designed and synthesized by Shanghai RIBOBIO Co., LTD (Guangzhou, China). IRF7-specific siRNA-2 (5′-CGAGCUGCACGUUCCUAUA dTdT-3′) was used to suppress endogenous IRF7 expression. ‘Nonsense’ sequence (5′-UUCUCCGAACGUGUCACGUdTdT-3′) was used as a control siRNA.

Sun L., Zhu Z., Cheng N., Yan Q, & Ye R.D. (2014). Serum amyloid A induces interleukin-33 expression through an IRF7-dependent pathway. European journal of immunology, 44(7), 2153-2164.

+ Open protocol

+ Expand

Macrophage Cell Transfection with siRNA

Check if the same lab product or an alternative is used in the 5 most similar protocols

The mouse macrophage cell line RAW264.7 was transfected with siRNA using a Silencer® siRNA Transfection II Kit (Ambion, Austin, TX) according to the manufacturer’s instructions. The HiPerFect transfection reagent (Qiagen) was used for siRNA transfection of human monocyte-derived macrophages. The cells were then cultured for 48 h before stimulation. The siRNA oligonucleotides were designed and synthesized by Shanghai RIBOBIO Co.,LTD (Guangzhou, China).

Sun L., Zhou H., Zhu Z., Yan Q., Wang L., Liang Q, & Ye R.D. (2015). Ex vivo and in vitro effect of serum amyloid A in the induction of macrophage M2 markers and efferocytosis of apoptotic neutrophils. Journal of immunology (Baltimore, Md. : 1950), 194(10), 4891-4900.

+ Open protocol

+ Expand

MRTF-A siRNA Regulation of Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols

Specific siRNA recognizing MRTF-A was purchased as predesigned siRNAs to MRTF-A (Dharmafect SMARTpool On TARGETplus MKL1 siRNA) alone or with a recommended non-target control siRNA (Ambion, Warrington, UK). Normal and SSc fibroblasts were transfected using Silencer siRNA Transfection II Kit (Ambion, Applied Biosystems, Warrington UK) with 60 nM siRNA. Cells were then used in floating collagen gel assay and for protein determinations by Western analysis.

Shiwen X., Stratton R., Nikitorowicz-Buniak J., Ahmed-Abdi B., Ponticos M., Denton C., Abraham D., Takahashi A., Suki B., Layne M.D., Lafyatis R, & Smith B.D. (2015). A Role of Myocardin Related Transcription Factor-A (MRTF-A) in Scleroderma Related Fibrosis. PLoS ONE, 10(5), e0126015.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!