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5 protocols using palcam base agar

1

Listeria Detection and Antimicrobial Analysis

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Tryptone soy broth (TSB), tryptone soy agar (TSA), Palcam base agar, Palcam selective supplement for Listeria, yeast extract, plate count agar (PCA), Dichloran Rose Bengali Chloramphenicol Agar (DRBC), and peptone were obtained from Biokar Diagnostics (Allonne, France), and Dew-Engley medium was obtained from Merck (Darmstadt, Germany).
Ascorbic acid, gallic acid, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), sodium carbonate, metaphosphoric acid, acetic acid, and 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) was obtained from Sigma-Aldrich (Steinheim, Germany). Methanol, acetone, chlorhidric acid (37%), sodium acetate, sodium hydroxide, sodium chloride, potassium chloride, ferric chloride hexahydrate, and Folin–Ciocalteu’s reagent were procured by Panreac (Llinars del Valles, Spain).
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2

Culturing Bacterial and Cell Lines

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All synthetic culture media, such as tryptone soy broth (TSB), tryptone soy agar (TSA), Palcam base agar, Palcam supplement, xylose‐lysine‐desoxycholate agar (XLD), yeast extract (YE), and peptone were obtained from Biokar Diagnostics (Allonne, France). Dey‐Engley broth was obtained from Fluka (Madrid, Spain). Dulbecco's modified Eagle medium (DMEM) and fetal bovine serum (FBS) were purchased from Hyclone (Pittsburgh, PA, USA); 200 mM glutamine, 10 mM N‐2‐hydroxyethylpiperazine‐N0‐2‐ethanesulfonic acid, and 1% penicillin–streptomycin were obtained from Biowest (Nuaillé, France).
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3

Strawberry Disinfection Protocols

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Strawberries (Fragaria × ananassa), were purchased from local distributors in Lleida (Catalonia, Spain) the day before the experiment and stored at 4 ± 1 °C for 24 ± 2 h.
For the disinfection, acetic acid 99-100 % w/v (AA) was purchased from Normapur VWR (Llinars del Vallés, Spain). Peracetic acid 15 % w/v (PA), sodium hypochlorite 10 % w/v (NaClO), hydrogen peroxide 33 % w/v (H2O2), pure anhydrous citric acid (CA) and pure lactic acid (LA) were procured by Panreac AppliChem (Barcelona, Spain). Triptone soy broth (TSB), triptone soy agar (TSA), plate count agar (PCA), dichloran rose bengale chloramphenicol agar (DRBC), PALCAM base agar, yeast extract (YE), Xylose-Lysine-Desoxycholate Agar (XLD) and peptone were purchased from Biokar Diagnostics (Allonne, France).
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4

Microbial Culture Media Preparation

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Tryptone soy broth (TSB), tryptone soy agar (TSA), PALCAM base agar, yeast extract (YE), Xylose-Lysine-Deoxycholate Agar (XLD) and peptone were purchased from Biokar Diagnostics (Allonne, France). Dey-Engley broth was obtained from Honeywell Fluka (Madrid, Spain). Sodium hypochlorite 10 % w v -1 (NaClO) was purchased by Panreac AppliChem (Barcelona, Spain). Dulbecco's modified Eagle medium (DMEM) and fetal serum bovine (FBS) was purchased from Hyclone (Pennsylvania, US).
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5

Strawberry safety and quality analysis

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Strawberries (Fragaria × ananassa) were purchased from local providers the same day of the experiment, and stored at 4 °C until it was carried out. Strawberries with no visible damages and similar in size were selected, and peduncle was removed.
Triptone soy broth (TSB), triptone soy agar (TSA), Palcam base agar and Palcam selective supplement for Listeria spp., yeast extract, plate count agar (PCA), dichloran rose bengale chloramphenicol agar (DRBC), sodium chloride and peptone were obtained from Biokar Diagnostics (Allonne, France). Dey-Engley broth was obtained from Honeywell Fluka (Madrid, Spain).
2,4,6-tris (2-pyridyl) -s-triazine (TPTZ), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and sodium carbonate were acquired from Sigma-Aldrich (Steinheim, Germany). Methanol, chlorhidric acid (37%), sodium acetate, sodium hydroxide, ferric chloride hexahydrate and Folin Ciocalteau's reagent were procured from Panreac (Llinars del Vallès, Spain).
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