Ascorbic acid, gallic acid, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), sodium carbonate, metaphosphoric acid, acetic acid, and 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) was obtained from Sigma-Aldrich (Steinheim, Germany). Methanol, acetone, chlorhidric acid (37%), sodium acetate, sodium hydroxide, sodium chloride, potassium chloride, ferric chloride hexahydrate, and Folin–Ciocalteu’s reagent were procured by Panreac (Llinars del Valles, Spain).
Palcam base agar
Palcam base agar is a microbiological culture medium used for the selective isolation and enumeration of Listeria species. It contains selective agents and supplements that inhibit the growth of most other bacteria, allowing Listeria species to be readily identified and counted.
Lab products found in correlation
5 protocols using palcam base agar
Listeria Detection and Antimicrobial Analysis
Ascorbic acid, gallic acid, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), sodium carbonate, metaphosphoric acid, acetic acid, and 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) was obtained from Sigma-Aldrich (Steinheim, Germany). Methanol, acetone, chlorhidric acid (37%), sodium acetate, sodium hydroxide, sodium chloride, potassium chloride, ferric chloride hexahydrate, and Folin–Ciocalteu’s reagent were procured by Panreac (Llinars del Valles, Spain).
Culturing Bacterial and Cell Lines
Strawberry Disinfection Protocols
For the disinfection, acetic acid 99-100 % w/v (AA) was purchased from Normapur VWR (Llinars del Vallés, Spain). Peracetic acid 15 % w/v (PA), sodium hypochlorite 10 % w/v (NaClO), hydrogen peroxide 33 % w/v (H2O2), pure anhydrous citric acid (CA) and pure lactic acid (LA) were procured by Panreac AppliChem (Barcelona, Spain). Triptone soy broth (TSB), triptone soy agar (TSA), plate count agar (PCA), dichloran rose bengale chloramphenicol agar (DRBC), PALCAM base agar, yeast extract (YE), Xylose-Lysine-Desoxycholate Agar (XLD) and peptone were purchased from Biokar Diagnostics (Allonne, France).
Microbial Culture Media Preparation
Strawberry safety and quality analysis
Triptone soy broth (TSB), triptone soy agar (TSA), Palcam base agar and Palcam selective supplement for Listeria spp., yeast extract, plate count agar (PCA), dichloran rose bengale chloramphenicol agar (DRBC), sodium chloride and peptone were obtained from Biokar Diagnostics (Allonne, France). Dey-Engley broth was obtained from Honeywell Fluka (Madrid, Spain).
2,4,6-tris (2-pyridyl) -s-triazine (TPTZ), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and sodium carbonate were acquired from Sigma-Aldrich (Steinheim, Germany). Methanol, chlorhidric acid (37%), sodium acetate, sodium hydroxide, ferric chloride hexahydrate and Folin Ciocalteau's reagent were procured from Panreac (Llinars del Vallès, Spain).
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