The LESA
extraction was controlled by a beta
version of the LESA Plus software (Advion, UK), and MassLynx 1.4 (Waters,
U.S.A.) was used for controlling the online washing step and the μLC
separation. Data were processed and visualized using Mass Lynx 1.4
(Waters, U.S.A.) and DriftScope v2.5 (Waters, U.S.A.). The identification
of the neuropeptides and proteins was performed using Progenesis QI
for proteomics v2.0.5556.29015 (Non Linear Dynamics, U.S.A.). For
this identification, a species-specific FASTA file was created, and
a nonspecific digest reagent was selected. The amount of missed cleavages
was set at three, and the post-translational modifications (PTMs)
allowed in this MSE search were N-acetylation, M-oxidation,
and C-carbamidomethylation.
extraction was controlled by a beta
version of the LESA Plus software (Advion, UK), and MassLynx 1.4 (Waters,
U.S.A.) was used for controlling the online washing step and the μLC
separation. Data were processed and visualized using Mass Lynx 1.4
(Waters, U.S.A.) and DriftScope v2.5 (Waters, U.S.A.). The identification
of the neuropeptides and proteins was performed using Progenesis QI
for proteomics v2.0.5556.29015 (Non Linear Dynamics, U.S.A.). For
this identification, a species-specific FASTA file was created, and
a nonspecific digest reagent was selected. The amount of missed cleavages
was set at three, and the post-translational modifications (PTMs)
allowed in this MSE search were N-acetylation, M-oxidation,
and C-carbamidomethylation.