Cyt c 10993 1 ap

Manufactured by Proteintech

Sourced in United States, China

Cyt-c (10993-1-AP) is a primary antibody produced by Proteintech. It is a highly specific antibody that recognizes cytochrome c, a key component of the electron transport chain in mitochondria.

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Lab products found in correlation

Ab215191, by Abcam (1 mentions) Bcl 2 15071, by Cell Signaling Technology (1 mentions) Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions) Mitotracker green fm, by Thermo Fisher Scientific (1 mentions) Mitochondria cytosol fractionation kit, by Abcam (1 mentions) Antimycin a, by Abcam (1 mentions) Ab14734, by Abcam (1 mentions) Ab191838, by Abcam (1 mentions) β tubulin, by Proteintech (1 mentions) Anti bax 50599 2 ig, by Proteintech (1 mentions) Nrf1 12482 1 ap, by Proteintech (1 mentions) Anti caspase3 9662, by Cell Signaling Technology (1 mentions) Gapdh 60004 1 ig, by Proteintech (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Ab32124, by Abcam (1 mentions)

3 protocols using cyt c 10993 1 ap

Western Blot Analysis of GBM Cells Treated with AT7519

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Western blot analysis of GBM cells treated with AT7519 was performed as described previously [32 ]. Antibodies against p-RB (T821) (ab32015), RB (ab181616), and GSDME (ab215191) were purchased from Abcam. Antibodies against p-CDK1 (T161) (9114), CDK1 (9116), Cyclin B1 (12231), Caspase-3 (14220), Cleaved Caspase-3 (9661), Cleaved PARP (5625), Caspase-9 (9502), MCL-1 (94296), and BCL-2 (15071) were purchased from Cell Signaling Technology. Antibodies against GAPDH (60004-1-Ig), PARP1 (13371-1-AP), p-P53 (S15) (28961-1-AP), P53 (10442-1-AP), and Cyt-c (10993-1-AP) were purchased from Proteintech. Antibodies against p-CDK2 (T160) (AP0325), CDK2 (A0094), Cyclin E1 (A12000), and NOXA (A9801) were purchased from ABclonal.

Zhao W., Zhang L., Zhang Y., Jiang Z., Lu H., Xie Y., Han W., Zhao W., He J., Shi Z., Yang H., Chen J., Chen S., Li Z., Mao J., Zhou L., Gao X., Li W., Tan G., Zhang B, & Wang Z. (2023). The CDK inhibitor AT7519 inhibits human glioblastoma cell growth by inducing apoptosis, pyroptosis and cell cycle arrest. Cell Death & Disease, 14(1), 11.

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Mitochondrial protein expression analysis

Cited 1 time

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Anti-COXIV (ab33985, RRID: AB_879754), VDAC1 (ab14734, RRID: AB_443084) and PGC-1α (ab191838, RRID: AB_2721267) antibodies were obtained from Abcam (Cambridge Science Park, Cambridge, UK). Anti-Caspase3 (9662, RRID: AB_331439) and cleaved-capase3 (9664, RRID: AB_2070042) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-Bax (50599-2-Ig, RRID: AB_2061561) , Cyt-c (10993-1-AP, RRID:AB_2090467) , NRF1 (12482-1-AP, RRID: AB_2282876), GAPDH (60004-1-Ig, RRID: AB_2107436), and β-tubulin (10094-1-AP, RRID: AB_2210695) antibodies were obtained from Proteintech (Rosemont, IL, USA). The anti-DsbA-L antibody was provided by Dr. Feng Liu(14). All secondary antibodies (MitoTracker Green FM and MitoTracker Red CMXRos) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). The calcium ionophore was purchased from Sigma-Aldrich (Shanghai, China). Antimycin A (>95% pure, ab141904) and a Mitochondria/Cytosol Fractionation Kit (ab65320) were purchased from Abcam (Cambridge Science Park, Cambridge, UK). The target sequence for mouse DsbA-L has been described previously.²² (link)

Li X., Pan J., Li H., Li G., Liu B., Tang X., Liu X., He Z., Peng Z., Zhang H., Wang L., Li Y., Xiang X., Chai X., Yuan Y., Zheng P, & Zhang D. (2022). DsbA-L interacts with VDAC1 in mitochondrion-mediated tubular cell apoptosis and contributes to the progression of acute kidney disease. EBioMedicine, 76, 103859.

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Apoptosis Pathway Protein Analysis

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Cells or hippocampi were lysed in radioimmunoprecipitation assay (RIPA) buffer containing phosphatase and protease inhibitor cocktails (Sigma). The experimental protocol was performed in accordance with a previous study [27 (link)]. Antibodies against Bax (50599-2-Ig; Proteintech, Wuhan, China), cytochrome c (Cyt c) (10993-1-AP; Proteintech), Bcl-2 (ab32124; Abcam), and cleaved caspase 3 (9661S; Cell Signaling Technology, Inc.) were purchased. β-Actin or cytochrome c oxidase (COXIV) antibody (66009-1-lg and 11242-1-AP; Proteintech) was used as internal controls.

Li Y., Yuan Y., Li Y., Han D., Liu T., Yang N., Mi X., Hong J., Liu K., Song Y., He J., Zhou Y., Han Y., Shi C., Yu S., Zou P., Guo X, & Li Z. (2021). Inhibition of α-Synuclein Accumulation Improves Neuronal Apoptosis and Delayed Postoperative Cognitive Recovery in Aged Mice. Oxidative Medicine and Cellular Longevity, 2021, 5572899.

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