Bm mscs

The human prostatic carcinoma cell lines, PC-3 and LNCaP, were purchased from American Type Culture Collection and cultured in RPMI-1640 medium (Gibco, USA) containing 10% fetal bovine serum (FBS, HyClone, USA), 100 Units/ml penicillin and 0.1 mg/ml streptomycin. Human primary cultures of bone marrow-derived MSCs (BM-MSCs) were purchased from Stemcell Technologies (Vancouver, BC) and cultured using the Human MesenCult® Proliferation Kit (Stemcell Technologies Inc). The adherent cells were cultured in a 5% CO₂ humidified environment at 37 °C and the medium was changed every 2–3 days. Only cells from passages three to six were used for the experiments.
An antagonist of CCR5 (Maraviroc) was purchased from Cayman Chemicals (Ann Arbor, Michigan, USA), and another antagonist of CCR5 (TAK-779) was synthesized by Takeda Chemical Industries (Osaka). Notch1 inhibitors LY3039478 and IMR-1 were obtained from Selleck (Houston, TX, USA).

BMMSCs were cultured in complete
Dulbecco’s Modified Eagle’s Medium-low
glucose (DMEM, Gibco, UK) supplemented
with 10% FBS, 100 U/ml penicillin, 100 U/ml
streptomycin, and 0.2 mmol/L glutamine at 37˚C
in a humidified incubator with an atmosphere
of 5% CO₂. The cells were passaged until 60%
confluency. The characterized BMMSCs were
prepared from StemCell Technology Company,
(Iran).