(1) Terpene profiling. Terpenoid analyses (DigiPath Laboratories) were carried out on an Agilent 7890B GC/7697A Headspace/5977A mass spectrophotometer with a DB-624UI and Agilent 5181–8818 split/splitless liner. Injector port temperature was 250°C with a transfer line, valve oven and needle temperature of 180°C. Carrier gas was helium at a flow of 33.0 cm/s. The MS detector was set to scan with a range from 50 to 300 m/z. The instrument was controlled by Agilent Masshunter quantitative analysis (Vers. B.08.00 Build 8.0.593.0). Certified reference standards were from Restek (Bellefonte, PA) with Masshunter library confirmation.
(2) Strain-inspired mixture: Cannabidivarin (CBDV), Cannabichromene (CBC), Cannabidiol (CBD), Cannabidiolic Acid (CBDA), Cannabigerol (CBG), Cannabigerolic Acid (CBGA), Cannabinol (CBN), alpha-Bisabolol, alpha-Humulene, alpha-Pinene, beta-Caryophyllene, beta-Myrcene, (+)-beta-Pinene, Camphene, Limonene, Linalool, Nerolidol. (3) Cannabinoid mixture: Cannabidivarin (CBDV), Cannabichromene (CBC), Cannabidiol (CBD), Cannabidiolic Acid (CBDA), Cannabigerol (CBG), Cannabigerolic Acid (CBGA), Cannabinol (CBN). (4) Terpene mixture: alpha-Bisabolol, alpha-Humulene, alpha-Pinene, beta-Caryophyllene, beta-Myrcene, (+)-beta-Pinene, Camphene, Limonene, Linalool, Nerolidol.
Jansen C., Shimoda L.M., Kawakami J.K., Ang L., Bacani A.J., Baker J.D., Badowski C., Speck M., Stokes A.J., Small-Howard A.L, & Turner H. (2019). Myrcene and terpene regulation of TRPV1. Channels, 13(1), 344-366.
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