Tumor tissue was fixed in 4% paraformaldehyde and embedded in paraffin. After sectioning, it was incubated with CD11b antibody (Bioss, China), CD14 antibody (Bioss, China), Ki67 antibody (Bioss, China), and LATS2 antibody (CST, USA) overnight at 4 °C. Then they were incubated with the second antibody and stained with diaminobenzidine. Photographs were taken with a light microscope.
Cd11b antibody
Manufactured by Bioss Antibodies
Sourced in China
The CD11b antibody is a laboratory reagent used to detect the CD11b protein, also known as the integrin alpha M chain or macrophage-1 antigen (Mac-1). CD11b is expressed on the surface of various immune cells, including monocytes, macrophages, and neutrophils. This antibody can be used in techniques such as flow cytometry and immunohistochemistry to identify and study these cell types.
Automatically generated - may contain errors
3 protocols using cd11b antibody
1
Immunohistochemical Analysis of Tumor Tissue
2
Histological Analysis of Tumor Tissues
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Tumour tissues were fixed with 4% paraformaldehyde (24 h), and embedded in paraffin blocks for routine histology. Haematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining were performed according to a standard procedure. Human monoclonal LDHB, KI67 and CD11b antibody (Bioss, China) for IHC were used at a 1:300 dilution. The pathological changes were assessed and photographed under Fluorescence Inversion Microscope System (OLYMPUS).
3
Immunohistochemical Analysis of Tumor Markers
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Tumor tissue was xed in 4% paraformaldehyde and embedded in para n. After sectioning, it was incubated with CD11b antibody (Bioss, China), CD14 antibody (Bioss, China), Ki67 antibody (Bioss, China) and LATS2 antibody (CST, USA) overnight at 4 ° C. Then they were incubated with the second antibody and stained with diaminobenzidine. Photographs were taken with a light microscope.
Nitroblue Tetrazolium (nbt) Assay NB4(1 × 10 5 cells/ml) was inoculated in a 6-well plate and treated with ATPR. A 10 µl aliquot of NBT solution, composed of 10 mg/ml NBT (Sigma-Aldrich) and 2 ug/ml PMA (Sigma-Aldrich), was added to each well, and then cells were incubated for 30 min at 37 °C. Then using light microscopy to analysis.
Nitroblue Tetrazolium (nbt) Assay NB4(1 × 10 5 cells/ml) was inoculated in a 6-well plate and treated with ATPR. A 10 µl aliquot of NBT solution, composed of 10 mg/ml NBT (Sigma-Aldrich) and 2 ug/ml PMA (Sigma-Aldrich), was added to each well, and then cells were incubated for 30 min at 37 °C. Then using light microscopy to analysis.
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