Captopril cap

Ang-[1–10], Ang-[1–8], Ang-[1–7], captopril (Cap), losartan, inactin, L-phenylephrine and Evans blue dye were purchased from Sigma (St. Louis, MO). Isoflurane was purchased from Cristália Ltda (Itapira, SP, Brazil). Ketoprofen (Ketoflex) was purchased from Mundo Animal (Pindamonhangaba, SP, Brazil). Penicillin, streptomycin, and dihydrostreptomycin were purchased from Fort Dodge Animal Health (Hialeah, FL, USA). Abz-Phe-Arg-Lys(Dnp)-Pro-OH was purchased from GenScript (Piscataway, NJ, USA). Mca-Ala-Pro-Lys(Dnp)-OH was purchased from Enzo Life Science (Farmingdale, NY, USA). MLN-4760 was purchased from EMD Milllipore (Billerica, MA, USA). Ceramic microbeads were purchased from MP Biomedicals (Solon, OH, USA). RNeasy was purchased from Qiagen (Germantown, MD, USA). RNase-free water, SYBR green supermix, and high-capacity cDNA reverse transcription kits (#1708890) were purchased from Bio-Rad (Hercules, CA, USA). Renin activity 520 Rat Renin Fluorimetric assay kit was purchased from SensoLyte (AnaSpec, San Jose-CA). Angiotensinogen Elisa kit was purchased from IBL (Hamburg, Germany). Aldosterone Elisa kit was purchased from Enzo Life Science (Farmingdale, NY, USA).

XYT includes seven traditional Chinese herbs: Astragalus membranaceus (Huang Qi), Epimedium sagittatum (Yin Yang Huo), Radix Ginseng Rubra, (Hong Shen), Leonurus japonicus (Yi Mu Cao), Ilex pubescens (Mao Dong Qing), Descurainiae Semen (Ting Li Zi), and Plantago asiatica (Che Qian Zi), all of which are recorded in the Chinese Pharmacopoeia. XYT (No. 20200901) was purchased from The First Affiliated Hospital of Guangzhou University of Chinese Medicine and approved by the Guangdong Pharmaceutical and Food Administration (No. Z20071257). The quality control of XYT has been established according to previous studies [21 (link)]. Captopril (Cap) was obtained from Sigma (USA). The following antibodies were purchased from CST (USA): anti-IL-6, anti-Bcl-2, anti-Bax, anti-TNF-α, and anti-caspase-3. All the other chemicals and reagents were of standard commercially available biochemical quality.