Log In Sign up
The largest database of trusted experimental protocols

Anti cd3ε m20

Manufactured by Santa Cruz Biotechnology
Visit Supplier
Sourced in Germany

Anti-CD3ε (M20) is a primary antibody that recognizes the CD3ε subunit of the T cell receptor complex. It is intended for research use only.

Automatically generated - may contain errors

Lab products found in correlation

Secondary antibodies for immunoblotting, by Merck Group (1 mentions) Anti zap70, by Cell Signaling Technology (1 mentions) Anti lck, by Cell Signaling Technology (1 mentions) Anti cd3ε okt3, by Thermo Fisher Scientific (1 mentions) Anti gapdh, by Merck Group (1 mentions) Las 4000 mini imager, by GE Healthcare (1 mentions)

2 protocols using anti cd3ε m20

1

T Cell Receptor Signaling Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used: anti-Nck1, anti-ZAP70, anti-Lck, anti-phospho-Lck (Src416) and anti-phospho-ZAP70 (Y319, Cell Signaling Technology), anti-phospho-ζ (Y142, Sigma-Aldrich), anti-idiotypic TCR (C305, Millipore), anti-CD3ε (M20, Santa Cruz Biotechnology), anti-CD3ε (OKT3, eBioscience), anti-ζ antiserum 448 [4 (link)], anti-phospho-CD3ε (Y188) [30 (link)], anti-GST (Bethly), anti-GAPDH (Sigma) and secondary antibodies for immunoblotting (Perbio). Alexa Fluor 647-labeled anti-CD3ε (UCHT1, BioLegend) was used for flow cytometry. Recombinant His-tagged Nck was from Sigma and recombinant active Lck (aa61-aa509) was a generous gift from B.F. Lillemeier, Salk Institute for Biological Studies, San Diego.
Hartl F.A., Ngoenkam J., Beck-Garcia E., Cerqueira L., Wipa P., Paensuwan P., Suriyaphol P., Mishra P., Schraven B., Günther S., Pongcharoen S., Schamel W.W, & Minguet S. (2021). Cooperative Interaction of Nck and Lck Orchestrates Optimal TCR Signaling. Cells, 10(4), 834.
+ Open protocol
+ Expand
2

T-cell Receptor Immunoblotting Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The immunoprecipitation eluates were separated by SDS–PAGE and detected by immunoblotting using standard protocols. Briefly, samples were subjected to SDS–PAGE separation, transferred to PVDF membranes for immunoblotting with anti-TCRα (H-1, Santa Cruz Biotechnology, Heidelberg, Germany), anti-TCRβ (H-197, Santa Cruz Biotechnology, Heidelberg, Germany), anti-CD3ε (M20, Santa Cruz Biotechnology, Heidelberg, Germany) and anti-ζ (a rabbit serum [51 (link)]). Immunoblots were developed using HRPO-conjugated secondary antibodies and detected by chemiluminescence on a LAS-4000 mini imager (GE Healthcare, Freiburg, Germany).
Yousefi O.S., Ruggieri M., Idstein V., von Prillwitz K.U., Herr L.A., Chalupsky J., Köhn M., Weber W., Timmer J, & Schamel W.W. (2021). Cross-TCR Antagonism Revealed by Optogenetically Tuning the Half-Life of the TCR Ligand Binding. International Journal of Molecular Sciences, 22(9), 4920.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!