BrdU (Fisher Scientific, # BP 2508250) was added to medium (3µg/ml) for 4 hrs, and fixed in 4% paraformaldehyde (PFA). Cells were incubated with α-BrdU (BD Biosciences, San Jose, CA, #347580) (1:1000), and then Alexa Fluor 594-conjuated Affinipure F (ab’)2 fragment goat anti-mouse IgG (H+L) secondary antibody (Jackson ImmunoResearch, West Grove, PA, code:115–586-003). Coverslips were mounted with Vectashield with DAPI (Vector, Burlingame, CA, H-1500). Images were obtained from 10 representative areas on each coverslip using a Leica DM2500 microscope with a DFC 365FX digital camera and the Leica Application Suite-Advanced Fluorescence software package (Leica Microsystems).
Alexa fluor 594 conjuated affinipure f ab 2 fragment goat anti mouse igg h l secondary antibody
Manufactured by Jackson ImmunoResearch
Alexa Fluor 594-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) is a secondary antibody that is labeled with the Alexa Fluor 594 fluorescent dye. It is designed to bind to the Fab portion of mouse immunoglobulin G (IgG) molecules, providing a means to detect and visualize mouse primary antibodies in various immunoassays.
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Lab products found in correlation
2 protocols using alexa fluor 594 conjuated affinipure f ab 2 fragment goat anti mouse igg h l secondary antibody
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BrdU Incorporation and Visualization
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BrdU Incorporation and Visualization
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BrdU (Fisher Scientific, # BP 2508250) was added to medium (3µg/ml) for 4 hrs, and fixed in 4% paraformaldehyde (PFA). Cells were incubated with α-BrdU (BD Biosciences, San Jose, CA, #347580) (1:1000), and then Alexa Fluor 594-conjuated Affinipure F (ab’)2 fragment goat anti-mouse IgG (H+L) secondary antibody (Jackson ImmunoResearch, West Grove, PA, code:115–586-003). Coverslips were mounted with Vectashield with DAPI (Vector, Burlingame, CA, H-1500). Images were obtained from 10 representative areas on each coverslip using a Leica DM2500 microscope with a DFC 365FX digital camera and the Leica Application Suite-Advanced Fluorescence software package (Leica Microsystems).
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