Biocoll solution

Manufactured by Harvard Bioscience

Sourced in Germany

Biocoll solution is a laboratory reagent used for the separation and isolation of cells and cellular components. It is a density gradient medium that allows for the separation of different cell types based on their density. The core function of Biocoll solution is to facilitate the isolation and purification of specific cell populations from complex biological samples.

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Lab products found in correlation

Li heparin, by Sarstedt (1 mentions) Sepmate, by STEMCELL (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) S monovette, by Sarstedt (1 mentions) Easysep human cd14 positive selection kit 2, by STEMCELL (1 mentions) Easysep human cd4 t cell isolation kit, by STEMCELL (1 mentions)

Close spelling variants of this product

Biocoll separating solution 1077 Biocoll separating solution Biocoll cell separation solution Biocoll separation solution Biocoll gradient solution Biocoll

6 protocols using biocoll solution

Extraction of PBMCs from Whole Blood

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Example 8

Human whole blood from healthy donors (in house) and whole blood from cynomolgus monkey (retrieved from LPT Laboratory of Pharmacology and Toxicology, Hamburg, Germany) was collected in Li-Heparin containing S-Monovette® (scientific laboratory instruments, apparatus and equipment for clinical use) containers (Sarstedt). Blood was transferred to 50 ml conical tubes and mixed with an equal volume of PBS containing 2% fetal bovine serum (Sigma, #F7524) and 2 mM EDTA. Diluted blood was transferred to SepMate® (cell separation equipment)-50 tubes (StemCell Technologies® (cell separation equipment), #86450) containing 15 ml Biocoll solution (Biochrom, #L6115) and centrifuged for 10 min at 1200×g. Supernatant was transferred into a 50 ml conical tube, diluted to 45 ml with PBS and centrifuged for 8 min at 300×g. Supernatant was discarded, cell pellet resuspended in 1 ml PBS and cells counted using a Neubauer chamber.

US11472880B2. Humanized antibodies for CD3 (2022-10-18). MORPHOSYS AG [DE]. Inventors: Thomas Tiller [DE], Steffen Runz [DE], Julia Neugebauer [DE], Andreas Bültmann [DE].

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Isolation of Human Monocytes from PBMC

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Peripheral Blood Mononuclear Cells (PBMC) were purified from whole blood by density gradient centrifugation using the Biocoll Solution (Cat No. 6715, Biochrom, Berlin, Germany) according to the manufacturer´s instructions. Monocytes were isolated from PBMCs by positive selection with magnetic beads using the EasySepTM Human CD14 Positive Selection Kit II (Cat No. 17858, Stemcell Technologies, Cologne, Germany) according to the manufacturer’s protocol.

Feiner L.K., Tierling S., Holländer S., Glanemann M, & Rubie C. (2021). An aging and p53 related marker: HOXA5 promoter methylation negatively correlates with mRNA and protein expression in old age. Aging (Albany NY), 13(4), 4831-4849.

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Longitudinal CLL Patient Blood Analysis

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Peripheral blood was obtained from 71 patients with CLL from 2007 to 2019 after confirmed diagnosis. Median time between diagnosis and sample acquisition was 4 years (interquartile range 2–9 years). All experiments have been approved by the appropriate ethics committee (Ethics Committee of the University of Tübingen vote 13/2007 V) and have therefore been performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments. Informed consent was obtained from all patients prior to initiation of experiments. Peripheral blood mononuclear cells (PBMC) were isolated using density gradient centrifugation using Biocoll Solution (Biochrom AG, Berlin, Germany) and thereafter stored in liquid nitrogen. Mean observational time for patients was 114 months (95% CI 96.8–132.6 months). Diagnosis was based on the iwCLL guideline [31 ]. Classification of CLL cases was performed according to the recent Rai and Binet classification systems [2 –5 (link)]. Cytogenetic analyses were performed with standardized methods at the medical care center (MVZ) Dortmund laboratory.

Greiner S.M., Märklin M., Holzmayer S., Kaban K., Meyer S., Hinterleitner C., Tandler C., Hagelstein I., Jung G., Salih H.R., Heitmann J.S, & Kauer J. (2022). Identification of CD105 (endoglin) as novel risk marker in CLL. Annals of Hematology, 101(4), 773-780.

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Isolation of Maternal Cells from Blood

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Maternal blood samples were immediately transferred into nonphysiological conditions and left overnight before separation by density gradient centrifugation as previously described (Sitar et al. 2005). Briefly, maternal blood samples were mixed with an equal volume of 1× medium‐199 with Earle salts (Sigma‐Aldrich, St Louis, MO) and immediately, 15% of ACD‐A was added to blood samples. The osmolarity of these solutions was adjusted to 320 mOsm/L using NaCl (20 mEq/10 mL).
Diluted blood samples were overlaid onto a Biocoll solution (Biochrom AG, Berlin, Germany) having a density of 1.072 g/L into a cell separation device. Centrifugation was run for 20 min at 400 × g. Cells floating at the interface were retrieved out of the separation device previously described (Sitar et al. 2005). Slides for FISH investigation were obtained by cytocentrifugation. The procedure took about 2 h with a capacity of four samples per run.

Calabrese G., Fantasia D., Alfonsi M., Morizio E., Celentano C., Guanciali Franchi P., Sabbatinelli G., Palka C., Benn P, & Sitar G. (2016). Aneuploidy screening using circulating fetal cells in maternal blood by dual‐probe FISH protocol: a prospective feasibility study on a series of 172 pregnant women. Molecular Genetics & Genomic Medicine, 4(6), 634-640.

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Isolating PBMCs from Blood Samples

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Blood of healthy men and of patients with depression was collected via venipuncture, diluted with PBS, carefully loaded on Biocoll solution (BioChrom, L6113), and centrifuged at 800 g for 20 min (brakeless running down). PBMCs were enriched by selecting the interphase of the Biocoll gradient. PBMCs of the interphase were washed two times with ice-cold PBS. PBMCs were resuspended in RPMI and plated at 4×10⁵ cells/cm². After recovery for 6 h, cells were treated with 888 nM (120 ng/ml) AMI, 1,695 nM (500 ng/ml) fluoxetine (FLX), or 365 nM (120 ng/ml) PAR. Concentrations had been chosen to match therapeutic concentrations in the serum according to the consensus guidelines for therapeutic drug monitoring in psychiatry [49] .

Gassen N.C., Hartmann J., Zschocke J., Stepan J., Hafner K., Zellner A., Kirmeier T., Kollmannsberger L., Wagner K.V., Dedic N., Balsevich G., Deussing J.M., Kloiber S., Lucae S., Holsboer F., Eder M., Uhr M., Ising M., Schmidt M.V, & Rein T. (2014). Association of FKBP51 with Priming of Autophagy Pathways and Mediation of Antidepressant Treatment Response: Evidence in Cells, Mice, and Humans. PLoS Medicine, 11(11), e1001755.

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Isolation of CD4+ T Cells from Peripheral Blood

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Peripheral blood was obtained from 3 healthy volunteers, two females and 1 male, aged between 25 and 42 years. Written informed consent was obtained from each participant and the protocol was supervised and approved by the Clinical Research Ethics Committee of the Alicante General Hospital (HGUA). Peripheral blood mononuclear cells (PBMCs) were isolated by gradient centrifugation using Biocoll solution (Biochrom, Berlin, Germany). CD4⁺ cells were then isolated from PBMCs by immunomagnetic selection using an EasySep^TM Human CD4⁺ T cell isolation kit (StemCell Technologies, Vancouver, BC, Canada).

Lozano-Ruiz B., Tzoumpa A., Martínez-Cardona C., Moreno D., Aransay A.M., Cortazar A.R., Picó J., Peiró G., Lozano J., Zapater P., Francés R, & González-Navajas J.M. (2022). Absent in Melanoma 2 (AIM2) Regulates the Stability of Regulatory T Cells. International Journal of Molecular Sciences, 23(4), 2230.

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