Plant aba elisa kit

ABA extraction was according to [83 (link)]. Briefly, Sclerotinia-infected leaf discs were collected and frozen in liquid nitrogen. After grinding with a pre-chilled mortar and pestle on ice, the powder was extracted overnight at 4°C in a cold extraction buffer (80% methanol and 2% glacial acetic acid). The mixture was then centrifuged at 2,000 g for 5 min. The supernatant was run through a Sep-Pak C18 Plus Short Cartridge (Waters Corp) to remove polar compounds. ABA content was measured by a plant ABA ELISA Kit (Jiancheng, Nanjing, China).
Tissue immuno-localization of ABA was according to [84 (link)]. Sclerotinia-infected leaves were fixed overnight with 3% (W/V) para-formaldehyde in 4% (W/V) 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide containing 0.1% (V/V) Triton X-100. Tissue cleaning was performed before infiltrating with wax. Sections (12 μm) were obtained with a sliding microtome (CM1850, Leica, Germany). After dewaxing and blocking, sections were incubated with rabbit anti-ABA primary antibody (Agrisera, Vännäs, Sweden) overnight. The fluorescence of Alexa 568 conjugated anti-rabbit secondary antibody was viewed with a confocal microscope (Zeiss LSM510 META).

The fruit ABA content was measured by a plant ABA ELISA Kit (Jiancheng, Nanjing, China). The sucrose content was determined by the plant tissue sucrose content detection kit (Jiancheng, Nanjing, China). The titratable acid (total acid) content was measured by phenolphthalein titration. Total anthocyanins were measured by the method depicted [45 (link)]. Each treatment included three replicates, and each repeat included 10 fruit individuals.