In a separate experiment, animals were randomly assigned into three groups: saline control (n = 11), 10 mg/kg (n = 9), or 20 mg/kg (n = 12) ketamine. Two 18F-FDG-PET and computed tomography (CT) scans were obtained for each animal at baseline, 4 to 5 days before the experiment (scan 1) and immediately after fear conditioning and ketamine infusion (scan 2). 18F-FDG-PET and CT images were acquired using an Inveon multimodality preclinical scanner (Siemens Medical Solutions, Erlangen, Germany) in the small animal imaging facility at USU as described previously [13 (link)]. Animals were briefly anesthetized with isoflurane (4% induction and 1.5–2.5% maintenance) and injected with 1.7 ± 0.153 mCi (62.9 ± 5.7 MBq) 18F-FDG through the tail vein. After 18F-FDG injection, the animal was returned to a clean cage during the uptake period (30 min) in a quiet room adjacent to the PET and CT scanner. Animals were undisturbed and exhibited minimal movement in their cages during the 18F-FDG uptake period. After uptake, animals were anesthetized with isoflurane (4% induction and 1.5 to 2.5% maintenance) to perform PET and CT scans. Physiologic monitoring during the scan included measurements of temperature, respiration rate, heart rate, and oxygen saturation.
Inveon multimodality preclinical scanner
Manufactured by Siemens
Sourced in Germany
The Inveon multimodality preclinical scanner is a medical imaging device designed for small animal research. It combines multiple imaging modalities, including positron emission tomography (PET), single-photon emission computed tomography (SPECT), and computed tomography (CT), to provide comprehensive imaging capabilities for preclinical studies.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using inveon multimodality preclinical scanner
1
PET-CT Imaging of Ketamine Effects
2
Insulin-Regulated Glucose Uptake in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Small-animal PET/CT was performed on a Siemens INVEON multimodality preclinical scanner. Mice (Dlk1+/+ n = 15, Dlk1−/− n = 13) were fasted overnight prior to PET/CT scanning at baseline (day 0) and one day later (day 1). Mice were subcutaneously injected with 75 μl saline or 0.75 U/(Kg body weight) short acting insulin (Humulin, NOVO Nordisk, DK) respectively, 15 min. Before injection of 18F-Fluoro-deoxy-glucose (FDG). Mice were anesthetized (2% isoflurane), and a bolus injection of FDG (day 0 95.5 ± 9.1 MBq; day 1: 91.6 ± 13.4 MBq) was administered via a tail vein catheter. Prior to the PET scan a two-bed CT scan was performed for attenuation correction of the PET data and anatomic orientation. CT and PET images were co-registered using a transformation matrix and CT-based attenuation correction was applied to the PET data. The PET data were reconstructed using the Siemens INVEON pre-clinical software and data analysis of the PET/CT fused images was performed with INVEON software version 4.2 (IRW, Siemens). FDG uptake in each volume of interest (VOI) was reported as standardized uptake values (SUV). VOIs of the skeletal limb hind muscles were normalized using brain uptake as a reference tissue that metabolizes glucose independent of insulin.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!