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Snap 5114

Manufactured by Bio-Techne
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Sourced in United Kingdom

SNAP-5114 is a handheld device designed for rapid and accurate measurement of molecular interactions. It utilizes surface plasmon resonance (SPR) technology to detect and quantify biomolecular binding events in real-time. The device is compact, easy to use, and suitable for a variety of applications in research and development.

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Lab products found in correlation

No 711, by Bio-Techne (2 mentions) Cucl2, by Merck Group (1 mentions) Agno3, by Merck Group (1 mentions) Mncl2, by Merck Group (1 mentions) Clampfit v10, by Molecular Devices (1 mentions) Cgp54626, by Bio-Techne (1 mentions) Sulforhodamine 101, by Thermo Fisher Scientific (1 mentions) Pluronic f 127, by Thermo Fisher Scientific (1 mentions) Oregon green bapta 1 am, by Thermo Fisher Scientific (1 mentions) Fluo 4 am, by Thermo Fisher Scientific (1 mentions) Bicuculline methiodide, by Merck Group (1 mentions)

8 protocols using snap 5114

1

Artificial Cerebrospinal Fluid Protocol

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Artificial cerebrospinal fluid (ACSF) contained in mM: 129 NaCl; 3 KCl; 1.6 CaCl2; 1.8 MgSO4; 1.25 NaH2PO4; 21 NaHCO3; 10 glucose. To induce epilepsy, MgSO4 was omitted, and 2 mM KCl was added (low-[Mg2+] ACSF). In the in vitro experiments, PUT (1 mM) was diluted in low-[Mg2+] ACSF. The pH value of 7.4 was not affected by the applied concentration. All solutions were continuously oxygenated (95% O2, 5% CO2). In the in vivo experiments on WAG/Rij rats, PUT (intraperitoneal/i.p. 25 mg/kg) and GABA (i.p. 300 mg/kg) were dissolved in saline. It was demonstrated previously that 1–30% dimethyl sulfoxide (DMSO) solution did not change the absence epileptic activity in WAG/Rij rats [37 (link)]; thus, SNAP-5114 (i.p. 20 mg/kg; TOCRIS, Bristol, UK) was dissolved in 10% DMSO solution. Unless otherwise stated, all drugs were purchased from Sigma-Aldrich, Budapest, Hungary, and Saint Louis, MO, USA.
Kovács Z., Skatchkov S.N., Szabó Z., Qahtan S., Méndez-González M.P., Malpica-Nieves C.J., Eaton M.J., Kardos J, & Héja L. (2022). Putrescine Intensifies Glu/GABA Exchange Mechanism and Promotes Early Termination of Seizures. International Journal of Molecular Sciences, 23(15), 8191.
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2

Neurophysiological Assessment of Divalent Cation Modulators

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Buffers contained in mM ACSF: 129 NaCl, 5 KCl, 1.6 CaCl2, 1.8 MgSO4, 1.25 NaH2PO4, 21 NaHCO3, 10 glucose (pH 7.4); nominally Mg2+-free ACSF was prepared as control ACSF with no added Mg2+ (we estimated the Mg2+ concentration of this buffer to be approximately 1 μM). Pharmacological assessment was done by the following compounds: MnCl2 (20 μM), AgNO3 (3.6 μM), CuCl2 (2 μM) – all purchased from Sigma-Aldrich, Schnelldorf, Germany, SNAP-5114 (100 μM, Tocris, Bristol, UK).
Szabó Z., Péter M., Héja L, & Kardos J. (2021). Dual Role for Astroglial Copper-Assisted Polyamine Metabolism during Intense Network Activity. Biomolecules, 11(4), 604.
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3

Induction of Epilepsy in Rat Models

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Artificial cerebrospinal fluid (ACSF) contained in mM: 129 NaCl; 3 KCl; 1.6 CaCl2; 1.8 MgSO4; 1.25 NaH2PO4; 21 NaHCO3; 10 glucose. To induce epilepsy, MgSO4 was eliminated and 2 mM KCl was added (low-[Mg2+] ACSF). In the in vitro experiments, SPM (SPM 200 μM) and FA sodium salt (1 mM) were diluted in ACSF or low-[Mg2+] ACSF. The pH value of 7.4 was not affected by the applied concentrations. All solutions were continuously oxygenated (95% O2, 5% CO2). In the in vivo experiments on WAG/Rij rats, levetiracetam (intraperitoneal/i.p. 200 mg/kg; TCI, Tokyo, Japan), DFMO (i.p. 150 mg/kg; TCI, Tokyo, Japan), 4-MCHA (i.p. 25 mg/kg), and APCHA (i.p. 25 mg/kg; TCI, Tokyo, Japan) were dissolved in saline. It was demonstrated previously that 1–30% dimethyl sulfoxide (DMSO) solution did not change absence epileptic activity in WAG/Rij rats (Kovács et al., 2011 (link)), thus, SNAP-5114 (i.p. 20 mg/kg; TOCRIS, Bristol, United Kingdom) was dissolved in 10% DMSO solution. Unless otherwise stated, all drugs were purchased from Sigma-Aldrich, Budapest, Hungary, and Saint Louis, MO, United States.
Kovács Z., Skatchkov S.N., Veh R.W., Szabó Z., Németh K., Szabó P.T., Kardos J, & Héja L. (2022). Critical Role of Astrocytic Polyamine and GABA Metabolism in Epileptogenesis. Frontiers in Cellular Neuroscience, 15, 787319.
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4

Optogenetic Manipulation of Striatal iMSN

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We used optical stimulation to activate iMSNs selectively. Channelrhodopsin-2 (ChR2) and its reporter gene enhanced yellow fluorescent protein (EYFP) were inserted in a double-floxed inverted open reading frame viral vector and stereotaxically injected into the striatum of 1 month-old WT and R6/2 mice crossed with A2A-Cre mice (1.0 mm anterior and 2.0 mm lateral to Bregma, at a depth of 3.1 mm from the dura). After Cre recombination, ChR2-EYFP was selectively expressed in A2A-Cre neurons. To examine evoked GABA responses in GPe neurons the following protocol was used: a slow ramp voltage command (3 s duration) from a holding voltage of −70 mV to +10 mV was first applied. Then the cell was held at the new voltage for 2 s, after which the optical stimulation (470 nm, 1 mW, 0.5 ms) was delivered. After 2 additional s at +10 mV the membrane potential was brought back to the original holding potential. This protocol was repeated 3 times (interval between sweeps was 30 s) and the responses to light stimulation were averaged. SNAP5114 (GAT-3 antagonist, 20 μM), NO711 (GAT-1 antagonist, 10μM), or CGP 54626 (GABAB receptor antagonist, 1μM) (all from Tocris, Minneapolis, MN) were bath applied (10-20 min) to examine alterations in decay time (90%-10%) of the evoked GABA response measured with Clampfit v10.7 (Molecular Devices, Sunnyvale, CA; RRID:SCR_011323).
Barry J., Sarafian T.A., Watson J.B., Cepeda C, & Levine M.S. (2020). Mechanisms Underlying the Enhancement of GABA Responses in the External Globus Pallidus of R6/2 Huntington’s Disease Model Mice. Journal of neuroscience research, 98(11), 2349-2356.
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5

Infusing SNAP5114 in GPe of Mice

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Mice were infused with 20 mM 1-[2-[tris(4-methoxyphenyl)methoxy]ethyl]-(S)-3-piperidinecarboxylic acid (SNAP5114, dissolved in dimethyl sulfoxide; Tocris) bilaterally in the GPe (AP −0.46 mm, ML ±2.0 mm, DV −3.2 mm from dura) using a cannula injection system (C313DC, C313G, and C313I; P1 Technologies) 20 min before the extinction test (28 (link)).
Kang S., Hong S.I., Kang S., Song M., Yang M.A., Essa H., Baker M., Lee J., Bruce R.A., Lee S.W, & Choi D.S. (2023). Astrocyte activities in the external globus pallidus regulate action-selection strategies in reward-seeking behaviors. Science Advances, 9(24), eadh9239.
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6

Inducing Epilepsy in ACSF

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Oxygenated (95% O2, 5% CO2) artificial cerebrospinal fluid (ACSF) contained in mM: 129 NaCl; 1.23 NaH2PO4; 10 glucose; 1.6 CaCl2.H2O; 3 KCl; 21 NaHCO3; 1.8 mM MgSO4. To induce epilepsy MgSO4 was eliminated and 2 mM KCl was added (low-[Mg2+] ACSF). Stock solutions of cell permeant Fluo-4 AM (2.5 mM, Life Technologies) and Oregon Green BAPTA-1 AM (OGB-1, 800 μM, Life Technologies) were diluted in 20% Pluronic F-127 (Life Technologies). Final DMSO concentration was 0.16% (Fluo-4) or 0.2% (OGB-1), which did not significantly altered epileptiform activity. Astrocytic γ-aminobutyric acid (GABA) transporter inhibitor SNAP-5114 (Tocris) stock solution (100 mM) was diluted in DMSO (final DMSO concentration in these experiments: 0.1%). Stock solution (50 mM, Sigma) of carbenoxolone hemisuccinate (CBX) was diluted in distilled water. Stock solution of astroglia-specific marker dye sulforhodamine101 (SR101, 10 mM, Invitrogen) was diluted in distilled water.
Kékesi O., Ioja E., Szabó Z., Kardos J, & Héja L. (2015). Recurrent seizure-like events are associated with coupled astroglial synchronization. Frontiers in Cellular Neuroscience, 9, 215.
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7

Synaptic Receptor Antagonists Protocol

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SNAP 5114 (GAT-3 blocker), NBQX (AMPAR blocker) and AP5 (NMDAR blocker) were purchased from Tocris Bioscience (R&D Systems, Inc., Minneapolis, MN, United States). Stocks of 100 mM SNAP 5114, 100 mM NBQX and 10 mM AP5 were made in DMSO or double distilled water and diluted in aCSF to reported concentrations. All other chemicals were purchased from Sigma−Aldrich (St. Louis, MO, United States) and Fisher Scientific (Pittsburgh, PA, United States).
Martinez D., Lima-Silveira L., Matott M.P., Hasser E.M, & Kline D.D. (2022). Gamma-Aminobutyric Acid Transporters in the Nucleus Tractus Solitarii Regulate Inhibitory and Excitatory Synaptic Currents That Influence Cardiorespiratory Function. Frontiers in Physiology, 12, 821110.
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8

Pharmacological Modulation of GABA Transporters

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Bicuculline methiodide was purchased from Sigma-Aldrich (St. Louis, MO). The GAT1 blocker NO-711 [(1,2,5,6-tetrahydro-1-[2-[[(diphenylmethylene)amino]oxy]ethyl]-3-pyridinecarboxylic acid hydrochloride)] and GAT3 blocker SNAP-5114 [1-[2-[tris(4-methoxyphenyl)methoxy]ethyl]-(S)-3-piperidinecarboxylic acid] were purchased from Tocris Bioscience (Minneapolis, MN).
Lu A.C., Lee C.K., Kleiman-Weiner M., Truong B., Wang M., Huguenard J.R, & Beenhakker M.P. (2020). Nonlinearities between inhibition and T-type calcium channel activity bidirectionally regulate thalamic oscillations. eLife, 9, e59548.
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