Immunohistochemical or immunocytochemical staining for 7 μm wax sections of ONHs or cultured ONH astrocytes were performed as described previously (Ju et al., 2008 (link)). Five sections per wax block from each group (n = 4 ONHs/group) were used for immunohistochemical analysis. The primary antibodies included mouse monoclonal anti-glial fibrillary acidic protein (GFAP) antibody (1:300; Sigma), guinea pig polyclonal anti-GFAP antibody (1:500; Advanced ImmunoChemical. Long Beach, CA), mouse monoclonal anti-neurofilament antibody (1:500; Sigma), mouse monoclonal anti-NR1 antibody (1:1000; BD Pharmingen, San Diego, CA), rabbit monoclonal anti-NR2A antibody (1:100; Millipore, Billerica, MA), rabbit polyclonal anti-NR2B antibody (1:5000; Millipore), and mouse monoclonal anti-DRP1 antibody (1:1000; BD Transduction Laboratories, San Diego, CA). The images were acquired with a FluoView1000 confocal microscope (Olympus, Tokyo, Japan).
Mouse monoclonal anti nr1 antibody
Manufactured by BD
Mouse monoclonal anti-NR1 antibody is a laboratory reagent used to detect the presence and quantity of the NR1 protein in biological samples. It is a mouse-derived antibody that specifically binds to the NR1 subunit of the NMDA receptor.
Automatically generated - may contain errors
Lab products found in correlation
Mouse monoclonal anti glial fibrillary acidic protein antibody, by Merck Group (2 mentions)
Mouse monoclonal anti neurofilament antibody, by Merck Group (2 mentions)
Rabbit monoclonal anti nr2a antibody, by Merck Group (2 mentions)
Rabbit polyclonal anti nr2b antibody, by Merck Group (2 mentions)
Mouse monoclonal anti drp1 antibody, by BD (2 mentions)
Fluoview 1000 confocal microscope, by Olympus (2 mentions)
2 protocols using mouse monoclonal anti nr1 antibody
1
Immunostaining of Optic Nerve Head Cells
2
Immunohistochemical Analysis of ONH Astrocytes
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Immunohistochemical or immunocytochemical staining for 7 µm wax sections of ONHs or cultured ONH astrocytes were performed as described previously (Ju et al., 2008 (link)). Five sections per wax block from each group (n = 4 ONHs/group) were used for immunohistochemical analysis. The primary antibodies included mouse monoclonal anti-glial fibrillary acidic protein (GFAP) antibody (1:300; Sigma), guinea pig polyclonal anti-GFAP antibody (1:500; Advanced ImmunoChemical. Long Beach, CA), mouse monoclonal antineurofilament antibody (1:500; Sigma), mouse monoclonal anti-NR1 antibody (1:1,000; BD Pharmingen, San Diego, CA), rabbit monoclonal anti-NR2A antibody (1:100; Millipore, Billerica, MA), rabbit polyclonal anti-NR2B antibody (1:5,000; Millipore), and mouse monoclonal anti-DRP1 antibody (1:1,000; BD Transduction Laboratories, San Diego, CA). The images were acquired with a FluoView1000 confocal microscope (Olympus, Tokyo, Japan).
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