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S7400

Manufactured by Merck Group
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Sourced in Germany

The S7400 is a laboratory equipment product manufactured by Merck Group. It is designed to perform precise measurement and analysis tasks in a research or laboratory setting. The core function of the S7400 is to provide accurate and reliable data collection and processing capabilities to support scientific investigations and experiments. No further details on the intended use or specific applications of this product can be provided in an unbiased and factual manner.

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Lab products found in correlation

Pluronic f 127, by Thermo Fisher Scientific (4 mentions) D sorbitol, by Merck Group (3 mentions) Fluozin 3 am, by Thermo Fisher Scientific (2 mentions) P4413, by Merck Group (2 mentions) A96606, by Merck Group (2 mentions) Paraformaldehyde (pfa), by Thermo Fisher Scientific (2 mentions) Nc0179595, by Thermo Fisher Scientific (2 mentions) Sodium arsenite, by Merck Group (2 mentions) Dtx 880 plate reader, by Beckman Coulter (1 mentions) P8139, by Merck Group (1 mentions) I3909, by Merck Group (1 mentions) A6419, by Merck Group (1 mentions) Tlrl 1826, by InvivoGen (1 mentions) Actinomycin d, by Merck Group (1 mentions) Pen strep solution, by Thermo Fisher Scientific (1 mentions) Pen strep solution, by Sartorius (1 mentions) Epilife medium, by Thermo Fisher Scientific (1 mentions) C11995500bt, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Sartorius (1 mentions) Dasatinib, by Merck Group (1 mentions)

27 protocols using s7400

1

Heat-Induced Renaturation of Fluorescent Proteins

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Heat-aggregated GFPuv and cfSGFP renaturation assays were performed as previously described (68 (link)) with modifications. The buffer contained sodium arsenite (Sigma–Aldrich, S7400) when indicated in the Figures, and it did not contain reducing agents. GFPuv was aggregated at 85 °C and cfSGFP at 77.8 °C for 15 min. Fluorescence was measured using the Beckman Coulter DTX 880 Plate Reader. Statistical analysis was performed using GraphPad Prism software (GraphPad Software Inc).
Hua S., Kłosowska A., Rodrigues J.I., Petelski G., Esquembre L.A., Lorentzon E., Olsen L.F., Liberek K, & Tamás M.J. (2022). Differential contributions of the proteasome, autophagy, and chaperones to the clearance of arsenite-induced protein aggregates in yeast. The Journal of Biological Chemistry, 298(12), 102680.
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2

Stimulation and Inhibition Protocols for Immune Cells

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Cells were treated with PMA (81 nM in human or 20 nM in murine studies; Sigma Aldrich; P8139) and ionomycin (1 μM; Sigma Aldrich; I3909) for indicated time points. Mepazine acetate (Vitas-M Laboratory Ltd; STK386548) was used at a concentration ranging between 6.5 and 20 μM. Sodium arsenite (Sigma Aldrich; S7400) was dissolved in complete medium at a final concentration of 1 M and used at 1 mM. Cultured monocytes were stimulated with 100 ng/mL LPS (Invivogen; tlrl-3pelps) or 5 mM ATP (Merck; A6419) dissolved in sterile H2O. For the chase experiments with actinomycin D (Merck; A9415), a final concentration of 5 μg/mL was used. Fifty micrograms of CpG ODN-1826 (Invivogen; tlrl-1826) was dissolved at a concentration of 500 μg/mL in sterile H2O and injected intraperitoneally every 2 days for 10 days. Ruxolitinib (ABCR; AB358151) was dissolved in 2.5% DMSO, 33% PEG400, and sterile H2O at a final concentration of 6.25 mg/mL. Mice were orally gavaged twice daily during 5 days with 1.25 mg of ruxolitinib or vehicle.
Tavernier S.J., Athanasopoulos V., Verloo P., Behrens G., Staal J., Bogaert D.J., Naesens L., De Bruyne M., Van Gassen S., Parthoens E., Ellyard J., Cappello J., Morris L.X., Van Gorp H., Van Isterdael G., Saeys Y., Lamkanfi M., Schelstraete P., Dehoorne J., Bordon V., Van Coster R., Lambrecht B.N., Menten B., Beyaert R., Vinuesa C.G., Heissmeyer V., Dullaers M, & Haerynck F. (2019). A human immune dysregulation syndrome characterized by severe hyperinflammation with a homozygous nonsense Roquin-1 mutation. Nature Communications, 10, 4779.
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3

Culturing Keratinocytes with Arsenite

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HaCaT human keratinocytes were obtained from the Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China). Cells were grown in DMEM medium (C11995500BT, GIBCO, Beijing, China) supplemented with 10% fetal bovine serum (FBS) (04-00101-A, Biological Industries, Cromwell, CT) and 1% pen-strep solution (03-031-1B, Biological Industries) under a humidified atmosphere of 5% CO2 and 95% air at 37°C. CD34high-enriched cells, CD34low-expressing cells, and passage-matched HaCaT (parent) cells were cultured on type IV collagen (3410-010-01, Trevigen, Gaithersburg, MD) and fibronectin-coated (610077, BD Biosciences, Bedford, MD) culture dish (or flask). Cells were maintained in EpiLife medium (MEPI500CA, Invitrogen, Shanghai, China) containing 5 ml of human keratinocyte growth supplement (S0015, Invitrogen) and 1% pen-strep solution. Cells at 80% confluence were exposed to sodium arsenite (S7400, Sigma, St. Louis, MO) as indicated.
Wu X., Yang B., Hu Y., Sun R., Wang H., Fu J., Hou Y., Pi J, & Xu Y. (2017). NRF2 Is a Potential Modulator of Hyperresistance to Arsenic Toxicity in Stem-Like Keratinocytes. Oxidative Medicine and Cellular Longevity, 2017, 7417694.
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4

Sodium Arsenite-Induced tRNAGLY Dysregulation

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EBV-positive wild-type BJAB-B1 or NSUN2 KO cells were treated with 0.5 mM sodium arsenite (SIGMA S7400) at a cell density of 5 × 105 cells/mL for the indicated time periods. RNA was extracted using TRIzol followed by northern blot analysis. The following probe was used to detect tRNAGLY as previously described (Yamasaki et al. 2009 (link)): 5′-GGCAGGCGAGAATTCTACCACTGAACCACCAA-3′;
Henry B.A., Kanarek J.P., Kotter A., Helm M, & Lee N. (2020). 5-methylcytosine modification of an Epstein–Barr virus noncoding RNA decreases its stability. RNA, 26(8), 1038-1048.
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5

Pharmacological modulation of cellular stress pathways

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GSK2606414 (TOCRIS #5107), CHX (Sigma-Aldrich #66819), dasatinib (Sigma-Aldrich CDS023389), ISRIB (Sigma-Aldrich SML0843), Tg (abcam #ab120286), osimertinib (LC labs, O-7200), Tm (abcam #ab120296), nelfinavir (Glentham Life Science #GP7332), lopinavir (Sigma-Aldrich #SML1222), sodium (meta)arsenite (Sigma-Aldrich #S7400), and cobalt chloride (Sigma-Aldrich #409332).
Mahameed M., Boukeileh S., Obiedat A., Darawshi O., Dipta P., Rimon A., McLennan G., Fassler R., Reichmann D., Karni R., Preisinger C., Wilhelm T., Huber M, & Tirosh B. (2020). Pharmacological induction of selective endoplasmic reticulum retention as a strategy for cancer therapy. Nature Communications, 11, 1304.
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6

Inducing Stress Granules in Cells

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To induce SGs, arsenite (S7400; Sigma-Aldrich; 500 µM), sorbitol (S1876; Sigma-Aldrich; 0.5 M), or hippuristanol (1 µM [Bordeleau et al., 2006 (link)]) was added, and the cells were incubated at 37°C for 1 h. To allow for faster stress recovery, cells were incubated with 100 µM arsenite for 1 h. For ribopuromycinylation assays, cells were incubated with puromycin (P8833; Sigma-Aldrich; 10 mg/ml) at 37°C for 5 min prior to fixation.
Ripin N., Macedo de Vasconcelos L., Ugay D.A, & Parker R. (2024). DDX6 modulates P-body and stress granule assembly, composition, and docking. The Journal of Cell Biology, 223(6), e202306022.
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7

Knockdown of SF3B1 and Arsenite Treatment

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Cells were transfected with small interfering RNAs (siRNAs; IDT, Israel) to knock down SF3B1 using Lipofectamine 2000 (Invitrogen, 11668). Arsenite treatment (0.25 mM, 30 min, Sigma, S7400) was applied 72 h after siRNA knockdown. Catalog numbers: 460262971, rArUrArUrUrGrArArGrCrArCrArGrA; 460262973, rGrArUrArCrGrGrUrGrArCrArUrUrCrArArU.
Angel M., Fleshler E., Atrash M.K., Kinor N., Benichou J.I, & Shav-Tal Y. (2024). Nuclear RNA-related processes modulate the assembly of cytoplasmic RNA granules. Nucleic Acids Research, 52(9), 5356-5375.
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8

Arsenite-Induced Stress Granule Formation

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To induce SG formation, HeLa cells were incubated 24 h posttransfection in culture medium supplemented with 0.5 mM sodium arsenite (Sigma-Aldrich, S7400) for 30 min at 37°C. Immediately after arsenite treatment, cells were treated for indirect immunofluorescent staining of the SG marker HuR using mAb 3A2 as primary antibody (dilution of 1:1000).
Diallo M.A., Pirotte S., Hu Y., Morvan L., Rakus K., Suárez N.M., PoTsang L., Saneyoshi H., Xu Y., Davison A.J., Tompa P., Sussman J.L, & Vanderplasschen A. (2022). A fish herpesvirus highlights functional diversities among Zα domains related to phase separation induction and A-to-Z conversion. Nucleic Acids Research, 51(2), 806-830.
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9

Zinc Regulation in Cellular Stress

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Cells were treated with 500 µM sodium arsenite (ARS) (NaAsO2; Sigma-Aldrich # S7400) for 1 hr prior to collection or fixing onto cover glasses. Cells were treated with indicated concentrations of TPEN (N,N,N′,N′-Tetrakis(2-pyridylmethyl)ethylenediamine) (10, 20, or 40 µM) (Sigma-Aldrich # P4413), azodicarbonamide (ADA; 50µM, Sigma-Aldrich #A96606) or 2,2′-dithiobisbenzamide-1 (DIBA-1; 100µM, Sigma-Aldrich #PZ0634) for 1hr prior to cell harvest. For visualization of Zn2+, cells were loaded with 1 µM FluoZin-3 AM (Thermo Fisher Scientific) and 0.02% Pluronic F-127 (Thermo Fisher Scientific # P3000MP) for 40 min, washed, and given fresh media. NC-LLPSs were treated with 500 µM ARS, 20 µM TPEN, or 3.5% 1,6-hexanediol (HEX) (Sigma-Aldrich #240117). The HIV-1 PR inhibitor saquinavir (SAQ) was obtained from the Division of AIDS, NIH through the NIH AIDS Research Reference and Reagent Program, and was used from the time of transfection to the time of collection.
Monette A., Niu M., Chen L., Rao S., James Gorelick R, & John Mouland A. (2020). Pan-retroviral Nucleocapsid-Mediated Phase Separation Regulates Genomic RNA Positioning and Trafficking. Cell reports, 31(3), 107520.
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10

Stress-Induced mRNA Localization Protocol

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To examine mRNA localization during stress, we used the following stress conditions. For arsenite stress experiments, cells were treated with 0.5 mM sodium arsenite (Sigma-Aldrich S7400) for 1 h. For osmotic stress, cells were stressed in 0.5M D-sorbitol (Sigma-Aldrich S1876) for 2.5 h. Cells were fixed after the completion of each stress with 4% paraformaldehyde (Fisher Scientific NC0179595).
Matheny T., Van Treeck B., Huynh T.N, & Parker R. (2021). RNA partitioning into stress granules is based on the summation of multiple interactions. RNA, 27(2), 174-189.
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