Icam 1

Manufactured by Jackson ImmunoResearch

Sourced in United States

ICAM-1 is a cell surface glycoprotein that functions as an intercellular adhesion molecule. It plays a role in the adhesion of cells to the extracellular matrix or to other cells.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

ICAM-1 KO mice (2 citations)

7 protocols using icam 1

Characterization of Monocyte Behavior

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Wild-type C57BL/6 mice in CD45.2 background or CD45.1 background were purchased from the National Cancer Institute (Frederick, MD). CX3CR1^gfp/gfp, CCR2^rfp/rfp, CD11a^-/-, CD11b^-/-, ICAM1^-/-, Nr4a1^-/-, and TNFR^-/- mice in C57BL/6 background were obtained from Jackson Laboratory. CX3CR1^gfp/gfp mice were bred with wild-type C57BL/6 mice or CCR2^rfp/rfp mice to produce CX3CR1^gfp/+ mice or CX3CR1^gfp/+CCR2^rfp/+ heterozygous mice. The CX3CR1^gfp/gfp mice express GFP under the promoter of the CX3CR1 gene [58 (link)], which is predominantly expressed on monocytes and has been extensively used to study monocyte behavior [11 (link), 12 (link), 32 (link)]. All colonies were maintained in ventilated specific-pathogen-free facilities with standard 12 h light/dark cycles. Animals between 6 and 12 weeks of age were used for all experiments.

Sun D., Zhang M., Sun P., Liu G., Strickland A.B., Chen Y., Fu Y., Yosri M, & Shi M. (2020). VCAM1/VLA4 interaction mediates Ly6Clow monocyte recruitment to the brain in a TNFR signaling dependent manner during fungal infection. PLoS Pathogens, 16(2), e1008361.

+ Open protocol

+ Expand

Tumor Growth Modeling in Genetically Engineered Mice

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

All mice (C57BL/6J, #0664; Foxn1−/−, #2019;
ICAM-1^−/−, #2867) were purchased from Jackson
Laboratory and allowed to acclimatize to local conditions for at least 1 week.
Animals were provided water and standard chow ad libitum and
were maintained on a 12-hour light/dark cycle. For tumor cell inoculation, a
100μL solution of 2 × 10⁵ of B16-F10 or 1 ×
10⁶ LLC was injected s.c. in the right rear leg of each mouse, as
described previously (11 (link)). Mice (sex- and
age-matched littermates) were randomly inoculated. Tumor volume was determined
by measuring the diameters of tumors with calipers and calculated by the
equation for volume of a spheroid: (a² × b ×
π)/6, where a is the short axis and bis the long axis of the tumor. Dysbiosis was induced 2 weeks prior to the tumor
inoculations by administering a cocktail of antibiotics, i.e. ampicillin (250
mg/L), vancomycin (125 mg/L), neomycin (250 mg/L), and metronidazole (250 mg/L)
in their drinking water, available ad libitum during the
experiment.(12 (link)) Mice receiving
TNF-α were randomized on day 7 after tumor inoculation, and murine
TNF-α treatment was initiated (q3dx4; 120 μg/kg in sterile PBS;
i.p.). After euthanization, organs were promptly harvested, measured, and
processed. Experiments were approved by the University of Arkansas for Medical
Sciences Institutional Animal Care and Use Committee (IACUC Protocol #3610 and
#3836).

Jenkins S.V., Robeson MS I.I., Griffin R.J., Quick C.M., Siegel E.R., Cannon M.J., Vang K.B, & Dings R.P. (2019). Gastrointestinal tract dysbiosis enhances distal tumor progression through suppression of leukocyte trafficking. Cancer research, 79(23), 5999-6009.

+ Open protocol

+ Expand

Transgenic Mouse Models for Immune Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

Wild type C57BL/6 mice were obtained from Charles Rivers. Transgenic mice, including CD11b^-/- (Cat# 003991), CD11a^-/- (Cat# 005257), ICAM-1^-/- (Cat# 002867), and CX3CR1^gfp/gfp (Cat# 005582), were purchased from Jackson lab and bred in specific pathogen-free (SPF) level animal facility of our institute. The mice were housed in controlled conditions with a temperature of 22-24°C, humidity of 30-60%, and a 12-hour light/12-hour dark cycle. Mice aged 6-12 weeks (both male and female) were used for all experiments. The animal study protocol was approved by the Animal Ethics Committee of Sun Yat-Sen University under protocol number [2018]08–81. The guidelines for the Ethical Review of Laboratory Animal Welfare (GB/T35892-2018) and institutional ethical guidelines for animal experiments were followed.

Zhu W., Zhang H., Dong Q., Song H, & Zhao L. (2023). Dual wave of neutrophil recruitment determines the outcome of C. albicans infection. Frontiers in Cellular and Infection Microbiology, 13, 1239593.

+ Open protocol

+ Expand

Murine Genetic Models for Inflammation Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

Male C57BL/6J, intercellular adhesion molecule-1 (Icam-1)^-/-, and p47^phox-/- mice were purchased from the Jackson Laboratories (Bar Harbor, ME) and housed in a temperature-controlled, 12-hour light/12-hour dark room. Cxcl1^-/- mice on a C57BL/6 background were kind gifts from Dr Sergio Lira (Mount Sinai, NY) and were further back-crossed into a C57BL/6J background for 5 more generations at the National Institute on Alcohol Abuse and Alcoholism animal facility, with C57BL/6J mice being used as wild-type (WT) controls. In some experiments, heterozygous breeding of Cxcl1^+/- mice was performed to obtain Cxcl1^-/- and littermate WT controls. Animal care was in accordance with the guidelines of the National Institutes of Health. The animal experiments were approved by the National Institute on Alcohol Abuse and Alcoholism Animal Care and Use Committee.

Zhou Z., Xu M.J., Cai Y., Wang W., Jiang J.X., Varga Z.V., Feng D., Pacher P., Kunos G., Torok N.J, & Gao B. (2018). Neutrophil–Hepatic Stellate Cell Interactions Promote Fibrosis in Experimental Steatohepatitis. Cellular and Molecular Gastroenterology and Hepatology, 5(3), 399-413.

+ Open protocol

+ Expand

Diabetic Skin Wound Healing in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

ICAM-1⁺/⁺ and ICAM-1^–/^– mice were obtained from the Jackson Laboratory (Bar Harbor, ME, United States). All mice were housed under specific pathogen-free conditions in full compliance with the Animal Use and Care Committee of Central South University, Changsha, Hunan Province, China. A type 1 diabetic model was induced by continuous low-dose streptozotocin (STZ) intraperitoneal injection (50 mg/kg; Sigma-Aldrich) for 5 days. The normal control (NG) was injected with an identical dose of phosphate-buffered saline (PBS). Mice were identified as diabetic based on a blood glucose level > 250 mg/dL. Skin injury was performed after the mice had maintained a diabetic status for longer than 3 weeks. Prior to surgery, mice were anaesthetized by intraperitoneal injection with a ketamine–xylazine solution (80 mg/kg ketamine, 5 mg/kg xylazine). We used a 3.0-mm biopsy punch to perform symmetrical full-thickness excisional injury on the skin. Mice were euthanized with CO₂ and injured tissue was collected 4 and 8 days after surgery. Seven mice per group were analyzed at each time point.

Qiu D., Zhang L., Zhan J., Yang Q., Xiong H., Hu W., Ji Q, & Huang J. (2020). Hyperglycemia Decreases Epithelial Cell Proliferation and Attenuates Neutrophil Activity by Reducing ICAM-1 and LFA-1 Expression Levels. Frontiers in Genetics, 11, 616988.

+ Open protocol

+ Expand

Murine Genetic Models for Immunoassays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Wild-type C57BL/6 and Mavs^–/–mice have been described previously⁵. ICAM-1^–/–mice on a C57BL/6 background were purchased from Jackson Laboratory (Stock No: 002867). All mice were housed and bread at the institutional animal facility under specific pathogen-free conditions. Mice were housed with standard housing in cages containing 3–5 mice per cage.

Cheng Y., Kiene N.J., Tatarian A., Eix E.F, & Schorey J.S. (2020). Host cytosolic RNA sensing pathway promotes T Lymphocyte-mediated mycobacterial killing in macrophages. PLoS Pathogens, 16(5), e1008569.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Intestinal Inflammation

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Small intestinal tissue from CLP and sham surgery from all three strains (WT, SIRT2KO, and SIRT2KI) of mice was harvested, and fixed frozen sections were stained using E-selectin and ICAM-1 (BD Biosciences, San Jose, CA, USA) and Von Willebrand factor (VWF, Abcam, Inc., Cambridge, MA, USA). Cy™3-conjugated labeled secondary antibodies for E-selectin, ICAM-1, and FITC-conjugated secondary antibody for VWF were purchased from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA, USA). The IHC techniques and virtual image captures were as described previously [22 (link), 23 (link)].

Buechler N., Wang X., Yoza B.K., McCall C.E, & Vachharajani V. (2017). Sirtuin 2 Regulates Microvascular Inflammation during Sepsis. Journal of Immunology Research, 2017, 2648946.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!