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N acetylcysteine nac a9165

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N-acetylcysteine (NAC; A9165) is a commonly used laboratory reagent. It is a derivative of the amino acid cysteine and serves as a precursor to the antioxidant glutathione. NAC is utilized in various research applications, including studies on oxidative stress and cell signaling pathways.

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Lab products found in correlation

3 2 4 dichloro 5 methoxyphenyl 2 3 dihydro 2 thioxo 4 1h quinazolinone mdivi 1 bml cm127, by Enzo Life Sciences (2 mentions) Cyp2e1 ab28146, by Abcam (1 mentions) Erk1 2 4695, by Cell Signaling Technology (1 mentions) Nox2 ab129068, by Abcam (1 mentions) Torin1, by Bio-Techne (1 mentions) Z vad fmk fmk001, by R&D Systems (1 mentions) Ciliobrevin a1, by Bio-Techne (1 mentions) Vismodegib gdc 0449, by Selleck Chemicals (1 mentions) Ab18766, by Abcam (1 mentions) Z vad fmk c1202, by Beyotime (1 mentions) Ab125066, by Abcam (1 mentions) Cuso4, by Chron Chemicals (1 mentions) Df4255, by Affinity Biosciences (1 mentions) 2 7 dichlorofluorescin diacetate dcfh da, by Beyotime (1 mentions) Edu assay kit, by RiboBio (1 mentions) Dq red bsa, by Thermo Fisher Scientific (1 mentions) Hyaluronic acid, by Maclin Biochemical Technology (1 mentions) Penicillin streptomycin solution, by Cytiva (1 mentions) Mitochondrial membrane potential assay kit with jc 1, by Beyotime (1 mentions) Annexin 5 fitc pi apoptosis detection kit, by Yeasen (1 mentions)

6 protocols using n acetylcysteine nac a9165

1

In Vitro Aging of Osteoblasts

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Osteoblasts were multiple-passaged as an in vitro model of age-induced bone loss, as previously described [26 (link),27 (link),28 (link)]. Upon euthanasia, primary (P0) calvarial osteoblasts were isolated from 3-month-old mice and incubated in DMEM and 10% FBS. Primary osteoblasts (5 × 105/well, 6-well plates) were incubated in medium for 3 days. After 10 passages, osteoblasts were designated to the aged group (P10). In some experiments, 105 cells/well (24-well plates) were incubated in 100 nM 5-Aza-2′-deoxycystidine (5-aza; A3656, Sigma-Aldrich, St Louis, MO, USA) or 10 μM N-acetylcysteine (NAC; A9165, Sigma-Aldrich, St Louis, MO, USA) for 4 h and incubated in an osteogenic medium.
Lian W.S., Wu R.W., Chen Y.S., Ko J.Y., Wang S.Y., Jahr H, & Wang F.S. (2021). MicroRNA-29a Mitigates Osteoblast Senescence and Counteracts Bone Loss through Oxidation Resistance-1 Control of FoxO3 Methylation. Antioxidants, 10(8), 1248.
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2

Investigating Hepatic Oxidative Stress Pathways

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Antibodies for GAPDH (3683), F4/80 (70076), phospho–NF–κB p65 Ser536 (3033), NF-κB p65 (8242), phospho-AKT Ser473 (4060), phospho-ERK1/2 Thr202/Tyr204 (4370) and ERK1/2 (4695) were from Cell Signaling Technology; AKT (sc-81434), phospho-eNOS Ser1177 (sc-21871-R), eNOS (sc-376751), iNOS (sc-7271), SOD1 (sc-271014) and GPx1/2 (sc-133160) were from Santa Cruz Biotechnology; CYP2E1 (ab28146), NOX2 (ab129068), NOX4 (ab216654) and 4-hydroxynonenal (4-HNE, ab46545) were from Abcam. Antibodies for murine (AF3954) and human (MABS197) PTP1B were from R&D Systems and Millipore, respectively. Information on the used antibodies is detailed in Supplementary Table 1. N-acetylcysteine (NAC; A9165) and hydrogen peroxide (H2O2; 386790) were from Sigma. PTP1B pharmacological inhibitor (DPM-1001) was from Glixx Laboratories (GLXC-11401). Dr. Zhaoli Sun (Johns Hopkins University) kindly provided de-identified human liver biopsies from healthy donors and alcoholic hepatitis patients.
Hsu M.F., Koike S., Mello A., Nagy L.E, & Haj F.G. (2020). Hepatic protein-tyrosine phosphatase 1B disruption and pharmacological inhibition attenuate ethanol-induced oxidative stress and ameliorate alcoholic liver disease in mice. Redox Biology, 36, 101658.
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3

Cellular Metabolism Modulation Compounds

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Bafilomycin A1 (B1793), Carbonyl cyanide m-chlorophenyl hydrazine (CCCP, C2759), rotenone (R8875), 1-methyl-4-phenylpyridinium (MPP+, D048), doxycycline (D9891), and N-acetyl-cysteine (NAC, A9165) were purchased from Sigma-Aldrich (St. Louis, MO). 3-(2,4-dichloro-5-methoxyphenyl)-2,3-dihydro-2-thioxo-4(1H)-quinazolinone (Mdivi-1, BML-CM127) was purchased from Enzo Life Sciences (Farmingdale, NY). Ciliobrevin A1 (#4529) and Torin-1 (#4247) were purchased from Tocris Bioscience (Bristol, UK). Vismodegib (GDC-0449) was purchased from Selleckchem (Munich, Germany). zVAD-FMK (FMK001) was purchased from R&D systems (Minneapolis, MN). 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, HY-15608) was obtained from MedChem Express (Monmouth Junction, NJ).
Bae J.E., Kang G.M., Min S.H., Jo D.S., Jung Y.K., Kim K., Kim M.S, & Cho D.H. (2019). Primary cilia mediate mitochondrial stress responses to promote dopamine neuron survival in a Parkinson’s disease model. Cell Death & Disease, 10(12), 952.
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4

Investigating Autophagy and Apoptosis Pathways

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CuSO4 came from Chengdu Kelong Chemical Co., Ltd. (Chengdu, China). N-acetylcysteine (NAC) (A9165) and 3-MA (M9281) were provided by Sigma Aldrich. Z-VAD-FMK (C1202) and 2′,7′-dichlorofluorescin diacetate (DCFH-DA) (S0033 M) were provided by Beyotime Biotechnology. Compound C (Com C) (S7306) was purchased from Selleck.
Antibodies involved in the current research included: rabbit anti-LC3B (2775S CST), rabbit anti-p62 (5114S CST), rabbit anti-AMPKα (5832 CST), rabbit anti-p-AMPKα (2535 CST), rabbit anti-mTOR (2972 CST), rabbit anti-p-mTOR (2971 CST), rabbit anti-ULK1 (8054 CST), rabbit anti-p-ULK1(Ser757) (6888 CST), rabbit anti-Atg16L1 (8089T CST), rabbit anti-Atg7 (8558 CST), rabbit anti-Atg3 (3415 CST), rabbit anti-Beclin1 (12994 CST), anti-rabbit cleaved-caspase-3 (9664 CST), anti-rabbit cleaved-caspase-8 (9496 CST), anti-rabbit cleaved-caspase-9 (9509 CST), anti-rabbit caspase-12 (ab18766 Abcam), and anti-rabbit cleaved-PARP (5625 CST), anti-rabbit GPX4 (ab125066 Abcam), anti-rabbit COX-2 (abs131986 Absin Biotechnology), anti-rabbit FTH1 (bs-5907R Bioss Biotechnology) and anti-rabbit NCOA4 (DF4255 Affinity biosciences).
Guo H., Ouyang Y., Yin H., Cui H., Deng H., Liu H., Jian Z., Fang J., Zuo Z., Wang X., Zhao L., Zhu Y., Geng Y, & Ouyang P. (2021). Induction of autophagy via the ROS-dependent AMPK-mTOR pathway protects copper-induced spermatogenesis disorder. Redox Biology, 49, 102227.
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5

Cytotoxicity and ROS Assay Protocol

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CLT (MED80120) was purchased from Medbio Pharmaceutical Technology Company. IR-820 (N119962) and hydroxychloroquine sulfate (H141480) were purchased from Shanghai Aladdin Biochemical Co., Ltd. Hyaluronic acid (H909935) was purchased from Shanghai Macklin Biochemical Co., Ltd. Penicillin-streptomycin solution (SV30010) was manufactured by HyClone Laboratories. Fetal bovine serum albumin (FBS) (FSP500) was purchased from ExCell Bio. Dulbecco’s modified Eagle’s medium (DMEM) (C11995500BT) was purchased from ThermoFisher Biochemical Products Co., Ltd. The ROS assay kit (S0033S), LDH assay (C0016), Calcein AM/PI cell viability/cytotoxicity assay kit (C2015S), and mitochondrial membrane potential assay kit with JC-1 (C2006) were provided by Beyotime Biotechnology. An Annexin V-FITC/PI apoptosis detection kit (40302ES20) was purchased from Yeasen Biotech Co., Ltd. EdU assay kit (C10310) was provided by Guangzhou RiboBio Co., Ltd. 3-(4,5-dimethyl-2-thiazoyl)-2,5-diphenyltetra tetrazolium bromide (MTT) (M2128) and N-acetyl cysteine (NAC) (A9165) were obtained from Sigma-Aldrich Co., Ltd. DQ-BSA Red (D-12,051) was purchased from ThermoFisher Scientific Co., Ltd. We thank the Pub-lab of West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University for providing several laboratory instruments.
Wang S., Zhou L., Tian H., Li B., Su M., Li Q., Nice E.C., Huang C., Shao J, & He T. (2023). Site-specific nanomodulator capable of modulation apoptosis for enhanced colorectal cancer chemo-photothermal therapy. Journal of Nanobiotechnology, 21, 24.
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6

Mitochondrial Dynamics Modulation Protocol

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Doxycycline (D9891) and N-acetylcysteine (NAC, A9165) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 3-(2,4-dichloro-5-methoxyphenyl)-2,3-dihydro-2-thioxo-4(1 H)-quinazolinone (Mdivi-1, BML-CM127) was purchased from Enzo Life Sciences (Farmingdale, NY, USA). MK-2206 (S1078) was purchased from Selleck Chemicals (Houston, TX, USA). For gene expression knockdown, cells were transfected with validated small inhibitory RNAs (siRNAs) targeting human HSPA9 (5′-AAACGCAAGUGGAAAUUAA-3′), Drp1 (5′-GAGGUUAUUGAACGACUCA-3′), or IFT88 (5′-CCGAAGCACUUAACACUUA-3′) using Lipofectamine 2000. The siRNAs were synthesized by Genolution (Seoul, Korea). At 48 h post-transfection, the cells were treated with the indicated reagents.
Bae J.E., Jang S., Kim J.B., Hyung H., Park N.Y., Kim Y.H., Kim S.H., Kim S.H., Ha J.M., Oh G.S., Park K., Jeong K., Jang J.S., Jo D.S., Kim P., Lee H.S., Ryoo Z.Y, & Cho D.H. (2023). Enhanced primary ciliogenesis via mitochondrial oxidative stress activates AKT to prevent neurotoxicity in HSPA9/mortalin-depleted SH-SY5Y cells. Molecular Brain, 16, 41.
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