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Il 17f pe 18f10

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IL-17F-PE (18F10) is a fluorescently labeled antibody used for the detection and analysis of IL-17F, a cytokine involved in inflammatory and autoimmune responses. This product is intended for research use only.

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Lab products found in correlation

Lsrfortessa, by BD (3 mentions) Il 17a percp 17b7, by Thermo Fisher Scientific (3 mentions) Gr 1 fitc, by Thermo Fisher Scientific (2 mentions) Zombie uv dye, by BioLegend (2 mentions) Cd4 apcef780, by Thermo Fisher Scientific (2 mentions) Brefeldin a, by GoldBio (2 mentions) F4 80 apcef780, by Thermo Fisher Scientific (2 mentions) Ly6c percp, by Thermo Fisher Scientific (2 mentions) Tcrβ pe, by Thermo Fisher Scientific (2 mentions) Cd8 ef450, by Thermo Fisher Scientific (2 mentions) Tnf bv650 mp6 xt22, by Thermo Fisher Scientific (2 mentions) Isotype control, by Thermo Fisher Scientific (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions) Brefeldin a, by Merck Group (1 mentions) Mp6 xt22, by Thermo Fisher Scientific (1 mentions) Live dead stain zombie uv dye, by BioLegend (1 mentions)

Close spelling variants of this product

IL-17F (28 citations) IL-17F PE (2 citations)

3 protocols using il 17f pe 18f10

1

Cytokine Expression Profiling of Hepatic Immune Cells

Cited 2 times
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Single cell suspensions were derived from hepatic homogenate and stained with directly conjugated monoclonal antibodies or isotype controls. Staining for cytokine expression was completed after 4 hours of stimulation with 50 ng/mL Phorbol 12-Myristate 13-acetate (PMA; Promega), 1 μg/mL Ionomycin (Calbiochem) and 10μg/mL Brefeldin A (Gold Bio). Data collection and analysis were done as previously described11 (link),63 (link). Briefly, cells were stained with Live/Dead stain (Zombie UV Dye: Biolegend) and with directly-conjugated monoclonal antibodies to CD45-AF700 (104), CD11b-PE (M1/70), F4/80-APCef780 (BM8), Ly6C-Percp (HK1.4), Gr1-FITC (RB6-8C5), NK1.1-PECy7 (PK136), TCRβ-PE (H57-597), CD4-APCef780 (GK1.5), CD8-ef450 (53-6.7), TNF-BV650 (MP6-XT22), IL-17A-Percp (17B7) and IL-17F-PE (18F10) [all antibodies from e-Bioscience]. Flow cytometry data were then collected using a LSR Fortessa (BD) flow cytometer and analyzed using FlowJo X software (vX0.7).
Giles D.A., Moreno-Fernandez M.E., Stankiewicz T.E., Graspeuntner S., Cappelletti M., Wu D., Mukherjee R., Chan C.C., Lawson M.J., Klarquist J., Sünderhauf A., Softic S., Kahn C.R., Stemmer K., Iwakura Y., Aronow B.J., Karns R., Steinbrecher K.A., Karp C.L., Sheridan R., Shanmukhappa S.K., Reynaud D., Haslam D.B., Sina C., Rupp J., Hogan S.P, & Divanovic S. (2017). Thermoneutral housing exacerbates non-alcoholic fatty liver disease in mice and allows for sex-independent disease modeling. Nature medicine, 23(7), 829-838.
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2

Hepatic Immune Cell Profiling

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Single cell suspensions were derived from hepatic digestion as described and stained with directly conjugated monoclonal antibodies or isotype controls (all eBioscience) [3 (link), 17 (link)]. To determine IL-17A and IL-17F production, total single cells were stimulated for 5 h with 50 ng/ml PMA (Sigma-Aldrich, St. Louis, MO) and 1 μg/ml Ionomycin (Calbiochem), in presence of brefeldin A (10 μg/mL, Sigma-Aldrich). Subsequently, flow cytometry was used to enumerate immune cell populations as described [18 (link)–20 (link)]. Briefly, cells were treated with FACS fix buffer (BD Biosciences) for 15 minutes, washed and incubated in PBS supplemented with 2% FBS and were subsequently stained with Live/Dead stain (Zombie UV Dye: Biolegend) and with directly-conjugated monoclonal antibodies to CD45-AF700 (104), TCRβ-PE (H57-597), CD4-APCef780 (GK1.5), CD8-ef450 (53–6.7), CD11b-PE (M1/70), CD11c-Percp (N418), F4/80-APCef780 (BM8), Ly6C-Percp (HK1.4) Gr1-FITC (RB6-8C5), NK1.1-PECy7 (PK136), B220-ef450 (RA3-6B2), IL-17RA-APC (PAJ-17R), TNF-BV650 (MP6-XT22), IL-17A-Percp (17B7) and IL-17F-PE (18F10) [all antibodies from e-Bioscience] for 30 minutes. Flow cytometry data were then collected using a LSR Fortessa (BD) flow cytometer and analyzed using FlowJo X software (vX0.7).
Giles D.A., Moreno-Fernandez M.E., Stankiewicz T.E., Cappelletti M., Huppert S.S., Iwakura Y., Dong C., Shanmukhappa S.K, & Divanovic S. (2016). Regulation of Inflammation by IL-17A and IL-17F Modulates Non-Alcoholic Fatty Liver Disease Pathogenesis. PLoS ONE, 11(2), e0149783.
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3

Cytokine Expression Profiling of Hepatic Immune Cells

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Single cell suspensions were derived from hepatic homogenate and stained with directly conjugated monoclonal antibodies or isotype controls. Staining for cytokine expression was completed after 4 hours of stimulation with 50 ng/mL Phorbol 12-Myristate 13-acetate (PMA; Promega), 1 μg/mL Ionomycin (Calbiochem) and 10μg/mL Brefeldin A (Gold Bio). Data collection and analysis were done as previously described11 (link),63 (link). Briefly, cells were stained with Live/Dead stain (Zombie UV Dye: Biolegend) and with directly-conjugated monoclonal antibodies to CD45-AF700 (104), CD11b-PE (M1/70), F4/80-APCef780 (BM8), Ly6C-Percp (HK1.4), Gr1-FITC (RB6-8C5), NK1.1-PECy7 (PK136), TCRβ-PE (H57-597), CD4-APCef780 (GK1.5), CD8-ef450 (53-6.7), TNF-BV650 (MP6-XT22), IL-17A-Percp (17B7) and IL-17F-PE (18F10) [all antibodies from e-Bioscience]. Flow cytometry data were then collected using a LSR Fortessa (BD) flow cytometer and analyzed using FlowJo X software (vX0.7).
Giles D.A., Moreno-Fernandez M.E., Stankiewicz T.E., Graspeuntner S., Cappelletti M., Wu D., Mukherjee R., Chan C.C., Lawson M.J., Klarquist J., Sünderhauf A., Softic S., Kahn C.R., Stemmer K., Iwakura Y., Aronow B.J., Karns R., Steinbrecher K.A., Karp C.L., Sheridan R., Shanmukhappa S.K., Reynaud D., Haslam D.B., Sina C., Rupp J., Hogan S.P, & Divanovic S. (2017). Thermoneutral housing exacerbates non-alcoholic fatty liver disease in mice and allows for sex-independent disease modeling. Nature medicine, 23(7), 829-838.
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