Amlodipine

Lercanidipine, Amlodipine, Nicardipine, Niguldipine, and Felodipine were purchased from Tocris (Tocris Bioscience, Bristol, UK). Bortezomib and Carfilzomib were from Selleckchem (Houston, TX, USA). Ixazomib (MLN9708) was purchased from Cayman Chemical (Ann Arbor, MI, USA). Necrostatin-1, 3-methyladenine (3-MA), bafilomycin A1, 2-bis(o-aminophenoxy)ethane-N, N, N’N’tetraacetic acid acetoxymethyl ester (BAPTA-AM), cycloheximide and ruthenium red (RR) were from Sigma-Aldrich (St Louis, MO, USA). Ru360 was from Calbiochem (EDM Millipore Corp., Billerica, MA, USA). MitoTracker-Red (MTR), propidium iodide (PI), Fluo-3-AM, Rhod-2-AM and tetramethylrhodamine methyl ester (TMRM) were purchased from Molecular Probes (Eugene, OR, USA). z-VAD-fmk was from R&D systems (Minneapolis, MN, USA). The primary antibody against ubiquitin (sc-8017), ATF4 (sc-200) and β-actin (sc-47778) and were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). p62 (610833) was purchased from BD sciences (San Jose, CA, USA). Cleaved caspase-3 (#9661), LC3B (#2775), PERK (#5683), p-eIF2α (#9721), eIF2α (#9722), and CHOP/GADD153 (#2895) were purchased from Cell Signaling Technology (Danvers, MA, USA). Secondary antibodies (rabbit IgG HRP(G-21234) and mouse IgG HRP (G-21040)) were from Molecular Probes.

PDGF-BB was provided by Peprotech (Hamburg, Germany). Imatinib mesylate, amlodipine, fasudile, calphostin C, SC51322, PF04418948, L798106 and L161982 were purchased from Tocris Bioscience (Ellisville, Missouri, USA). Ponatinib was acquired from SelleckChem (Munich, Germany). SQ29548, RO-1138452, SU6668, PD98059 and U0126 were acquired from Cayman Europe (via Biomol, Hamburg, Germany). ET-1 was purchased from Biotrends (Wangen, Switzerland). Cytochalasin D or standard laboratory chemicals were provided by Sigma (Steinheim, Germany).

Pentobarbital (Narcoren) was purchased from Merial (Hallbergmoos, Germany), gelatin from porcine skin from Sigma-Aldrich and low melting point agarose from GERBU (Heidelberg, Germany). ET-1 was purchased from BIOTRENDS (Wangen, Switzerland). Glibenclamide, iberiotoxin, 4-aminopyridine and amlodipine were purchased from Tocris Bioscience (Ellisville, Missouri, USA). Human PDGF-BB was provided by Peprotech (Hamburg, Germany). Imatinib and nilotinib were kindly provided by Novartis (Basel, Switzerland). Standard laboratory chemicals were obtained from Sigma-Aldrich (Steinheim, Germany). The ELISA-kits were acquired from Enzo (Lörrach, Germany).