Cell numbers and viabilities were determined using a Neubauer chamber and trypan blue (Life Technologies) exclusion in phase-contrast microscopy (Zeiss Invertoskop C, Carl Zeiss, Jena, Germany).
Invertoskop c
Manufactured by Zeiss
Sourced in Germany
The Zeiss Invertoskop C is a laboratory microscope designed for inverted imaging. It is primarily used for the observation and examination of cells and small biological samples. The Invertoskop C provides a stable and reliable platform for various microscopy techniques, enabling researchers to conduct detailed analyses of their specimens.
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4 protocols using invertoskop c
1
Cell Viability Determination Protocol
2
Hematopoietic Progenitor Colony Assay
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Colony forming unit (CFU) assays were performed to investigate the potential of different hematopoietic progenitors to form colonies of various hematopoietic lineages. Cells were cultured in ultralow attachment dishes (STEMCELL Technologies, Vancouver, Canada). A complete MethoCult methylcellulose-based assay was used according to the manufacturer's instructions (STEMCELL Technologies). For the investigation of effects of DLK1 knockdown, cells were collected after five days of incubation with siRNA and added to methylcellulose. For the investigation of the effects of physical contact of Dlk1+ cells on hematopoietic cells (as described above), Dlk1− cells were collected after five days of culture and added to methylcellulose. After 14 days of culture, colonies were classified and counted on gridded dishes using phase microscopy (Zeiss Invertoskop C, Carl Zeiss, Jena, Germany). Four different types of colonies were identified, including the CFU-E (colony forming unit-erythrocyte), BFU-E (burst forming unit-erythrocyte), CFU-GM (colony forming unit-granulocyte and macrophage), and CFU-GEMM (colony forming unit-granulocyte, erythrocyte, macrophage, and megakaryocyte) colonies.
3
Colony Forming Potential of HUVECs
4
Cell Counting and Viability
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The total cell numbers and viabilities of freshly isolated cells were determined using a Neubauer chamber and trypan blue (Thermo Fisher Scientific) exclusion in phase contrast microscopy (Zeiss Invertoskop C, Carl Zeiss, Jena, Germany).
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