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Il 21 pe

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IL-21-PE is a fluorescently-labeled antibody that binds to the human Interleukin-21 (IL-21) protein. It is commonly used in flow cytometry applications to detect and quantify IL-21 expression in biological samples.

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Lab products found in correlation

Ifn γ pe cy7, by BD (4 mentions) Il 2 bv711, by BD (3 mentions) Cd40l bv605, by BioLegend (3 mentions) Cxcr5alexa647, by BD (2 mentions) Cd45rope texasred, by Beckman Coulter (2 mentions) Lsrfortessa, by BD (2 mentions) Pd 1 bv650, by BD (2 mentions) Il 22 pe, by Thermo Fisher Scientific (2 mentions) Ifn γ fitc, by BD (2 mentions) Cd45 apc h7, by BD (2 mentions) Cd56 pe cy7, by BD (2 mentions) Il 22 apc, by Thermo Fisher Scientific (2 mentions) Cd8α v500, by BD (2 mentions) Il 17a v450, by BD (2 mentions) Il 6 percp, by Thermo Fisher Scientific (2 mentions) T bet pe cy7 clone 4b10, by Thermo Fisher Scientific (2 mentions) Cd8α fitc, by BD (2 mentions) Cd49b pe clone dx5, by BD (2 mentions) Cd19 fitc, by BD (2 mentions) Facsverse, by BD (2 mentions)

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IL-21 (245 citations)

8 protocols using il 21 pe

1

Flow Cytometry of Immune Cell Subsets

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The following fluorochrome conjugated antihuman monoclonal antibodies (MoAbs) were used for flow cytometry studies: ICOSBV421, ICOSAlexa488, CD40LBV605, CD69BV650, HLA-DRFITC, CD38APCCy7, and TNF-αAPCCy7 from BioLegend (San Diego, CA); CD3BUV395, CD4PerCPCy5.5, CD8Alexa-Fluor700, CCR7PECF594, IL-2BV711, CXCR5Alexa647, IFNγPE-Cy7, PD1BV650, CD45ROAPCH7, CD21PECy5, CD27PerCPCy5.5, IgDFITC, CD10PECy7, and CD20Alexa700 from BD Bioscience (San Jose, CA); IL-21PE, CD27PECy5, and IL-17Alexa488 from e-Biosciences (San Diego, CA); and CD45ROPE-TexasRed from Beckman Coulter (Fullerton, CA). Live/Dead Fixable Aqua Dead Cell Stain Kit and CellTrace Violet Cell Proliferation Kit were from ThermoFisher (Boston, MA). Recombinant human IL-21 (Cat #8879-IL-010) from R&D systems, purified antihuman IL-2 (Cat #3440-ON-500) and antihuman-IL-21 (Cat #MT216G/21.3m) from Mabtech, and antihumanTNF-α (Cat #502922) from BioLegend were used. Samples were acquired on a BD LSRFortessa (BD Biosciences, CA) flow cytometer and analyzed by FlowJo V10 (TreeStar, Inc).
Pallikkuth S., de Armas L.R., Rinaldi S., George V.K., Pan L., Arheart K.L., Pahwa R, & Pahwa S. (2019). Dysfunctional peripheral T follicular helper cells dominate in people with impaired influenza vaccine responses: Results from the FLORAH study. PLoS Biology, 17(5), e3000257.
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2

Comprehensive Immunophenotyping of T and B Cells

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Phenotypic analyses of T cells and B cells were performed with anti-human monoclonal antibodies (mAbs): anti-human CD3-PerCP, CD4-FITC, CD19-PerCP and CD21-APC were from BD Biosciences (San Jose, CA, USA). CXCR5-APC, ICOS-PE, PD-1-PE, IFN-γ-PE, IL-4-PE, IL-17-PE, IL-21-PE, IL-22-PE, CD27-FITC, CD86-PE, CD95-PE, CD25-APC, and Foxp3-PE antibodies were obtained from eBiosciences (San Diego, CA, USA). Cells were analyzed by flow cytometry (BD FACSCalibur, San Jose, CA) and data was analyzed by FlowJo software (Tree Star, San Carlos, CA).
Kong F., Zhang W., Feng B., Zhang H., Rao H., Wang J., Cong X, & Wei L. (2015). Abnormal CD4 + T helper (Th) 1 cells and activated memory B cells are associated with type III asymptomatic mixed cryoglobulinemia in HCV infection. Virology Journal, 12, 100.
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3

Flow Cytometry Analysis of Tumor-Infiltrating Lymphocytes

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Flow cytometry analysis was performed by using a BD FACSVerse (BD Biosciences, Milan, Italy) and the following monoclonal anti-human antibodies: CD45-APC-H7, CD3ɛ-PerCP, CD3ɛ-FITC, CD8α-V500, CD56-V450, CD56-PE-Cy7, CD19-FITC, IFN-γ-FITC, IFN-γ-PE, IL-17A-V450, IL-17F-PE, TNFα-PE (all from BD Pharmingen, Milan, Italy), IL-21-PE, IL-22-APC, IL-6-PerCP, T-bet-PE-Cy7 (clone 4B10), RORγt-APC (clone AFKJS-9), RORγt-PE (clone AFKJS-9) (all from eBioscience) and CD68 (BioLegend, San Diego, CA, USA). TILs isolated from mouse colon tumors were stained with the following antibodies: CD3ɛ-Pacific Blue, CD8α-FITC, CD45-APC-Cy7, CD49b-PE (clone DX5), CD19-APC (all from BD Pharmingen) and F4/80-Pacific Blue (BioLegend). In parallel, cells were stained with the respective control isotype antibodies.
De Simone V., Franzè E., Ronchetti G., Colantoni A., Fantini M.C., Di Fusco D., Sica G.S., Sileri P., MacDonald T.T., Pallone F., Monteleone G, & Stolfi C. (2014). Th17-type cytokines, IL-6 and TNF-α synergistically activate STAT3 and NF-kB to promote colorectal cancer cell growth. Oncogene, 34(27), 3493-3503.
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4

Comprehensive Immunophenotyping of B and T Cells

Cited 1 time
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Following specific stimulation, PBMCs were stained for B cell phenotype with the following monoclonal antibodies (mAbs, from BD Biosciences): CD3, CD10, CD16 (BV510), CD19 (APC-R700), CD21 (APC), CD27 (FITC), CD38 (PE-Cy7), CD73 (PErCP-Cy5.5), CD80 (APC-H7) IgD (BV421), IgM (PE-CF594), IgG (BV605, HIV-1 envelope trimeric gp140 protein (gp140-R-Phycoerythrin (-R-PE)) and Live/Dead (BV510; BD Horizon-). T cell phenotype using the following mAbs: CD3 (BUV496), CD4 (APC-Cy7), CD27 (BV480), CXCR5 (Alexa647), IFNγ (PE-Cy7), IL-2 (BV711) and TNFα (FITC) from BD Biosciences, CD8 (PerCP) and CD40L (BV605) from BD BioLegend, IL-21 (PE, eBioscience), CD45RO (PE-TexasRed, Beckman Coulter) and Live/Dead® Fixable Blue Dead Stain Kit (ThermoFisher). The gp140 protein was obtained from European Research Infrastructures for Poverty Related Disease (EURIPRED) and Lightning-Link® R-PE Conjugation Kit (Innova Biosciences) was used to obtain a labelled gp140-R-PE. Cells were acquired on a BD LSRFortessa- and analyzed by FlowJo v10.0.8r1 (Treestar,Inc). Sorting was performed as shown in Supplementary Figure 2 using FACSAriaII (BD Biosciences); sorted cells were collected in pre-labelled tubes containing reverse transcription buffer for consequent PCR prior to Fluidigm [11 (link)]
COTUGNO N., MORROCCHI E., RINALDI S., ROCCA S., PEPPONI I., CESARE S.D., BERNARDI S., ZANGARI P., PALLIKKUTH S., ARMAS L.D., LEVY O., ROSSI P., PAHWA S, & PALMA P. (2020). Early ART-treated perinatally HIV-infected seronegative children demonstrate distinct long-term persistence of HIV-specific T and B cell memorys. AIDS (London, England), 34(5), 669-680.
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5

Comprehensive Immune Profiling of TILs

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Flow cytometry analysis was performed by using a BD FACSVerse (BD Biosciences). Human TILs were stained with the following monoclonal antibodies: CD45-APC-H7, CD3ε-PerCP, CD8α-V500, CD56-PE-Cy7, CD19-FITC, IFN-γ-FITC, IL-17A-V450 (all from BD Pharmingen, Milan, Italy), IL-21-PE, T-bet-PE-Cy7 (clone 4B10), RORγt-APC (clone AFKJS-9), Foxp3-FITC (clone PCH101), IL-22-APC, IL-6 PerCP (both from eBioscience), CD68-APC (Biolegend, San Diego, CA). Mouse LPMCs and TILs were stained with the following monoclonal antibodies: CD45-APC-Cy7, CD4-PerCP, CD8α-FITC, CD49b-PE (clone DX5), FoxP3-APC, IL-17A-PE, IFN-γ-PE-Cy7 (both from BD Pharmingen), perforin-APC, granzyme B-PE-Cy7 (all from eBioscience). In parallel, cells were stained with the respective control isotype antibodies.
De Simone V., Ronchetti G., Franzè E., Colantoni A., Ortenzi A., Fantini M.C., Rizzo A., Sica G.S., Sileri P., Rossi P., MacDonald T.T., Pallone F., Monteleone G, & Stolfi C. (2015). Interleukin-21 sustains inflammatory signals that contribute to sporadic colon tumorigenesis. Oncotarget, 6(12), 9908-9923.
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6

Comprehensive Immune Cell Profiling

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All antibodies were used at the concentration recommended by the manufacturer or pre-titrated for optimal staining. The following antibodies were used in this study; anti-CD3 APC-eFluor 780 (UCHT1), -CD4 APC (OKT4), -CD161 PE-Cy7 (HP-3G10), -IFNγ eFluor450 or PE (45.B3), -IL-17A eFluor 488 (eBio64DEC17), -IL-22 PE (22URT1), -IL-21 PE (eBio3A3-N2), -IL-17F PE (SHLR17), -T-bet PE (eBio4B10), -RORγt PE (AFKJS-9) (eBioscience), anti-CD3 Brilliant Violet 510 (OKT 3), -CD4 PE-Cy7 (OKT4), -CD8 Brilliant Violet 510 (RPA-T8), -CD25 PerCP-Cy5.5 (BC96), -CCR6 PE or Alexa Fluor (AF) 647 (G034E3), -CCR7 AF488 (G043H7), -CXCR3 AF647 (G025H7), anti-CD45RA PE-CF594 (HI100), -CD45RO PE-CF594 (UCHL1), -GM-CSF PE (BVD2-21C11) (BD Pharmingen), and anti-CD4 PE-Vio770 (VIT 4) (Miltenyi Biotec).
Restorick S.M., Durant L., Kalra S., Hassan-Smith G., Rathbone E., Douglas M.R, & Curnow S.J. (2017). CCR6+ Th cells in the cerebrospinal fluid of persons with multiple sclerosis are dominated by pathogenic non-classic Th1 cells and GM-CSF-only-secreting Th cells. Brain, Behavior, and Immunity, 64, 71-79.
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7

Multiparameter Flow Cytometry Analysis

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The following fluorochrome-conjugated anti-human mAb were used for flow cytometry studies: LAG3 BV650, TIGIT PE-Cy7, CTLA4 PE, CD25 PE-Dazzle 594, TIM3 PerCP-Cy5.5, CD19 Alexa Fluor 700, CCR4 BV421, ICOS PE-Cy7, CCR5 PE-Dazzle 594, HLA-DR PE, CCR7 FITC, CD38 BV711, PD-L1 BV711, CCR6 Alexa Fluor 700, PD-1 BV421, CD40L BV605, perforin PE-Dazzle 594, and CD8 PerCP from BioLegend (San Diego, CA); CD3 BUV496, CD4 APC-Cy7, CD4 APC-H7, CD127 BV605, Ki-67 PerCP-Cy5.5, PD-1 BV650, CXCR3 BV605, CD69 BV650, IL-2 BV711, CXCR5 Alexa Fluor 647, IFN-γ PE-Cy7, TNF-α FITC, granzyme B Alexa Fluor 700 and CD27 BV480 from BD Biosciences (San Jose, CA); IL-21 PE from eBioscience, (San Diego, CA); and CD45RO PE-Cy5.5 from Beckman Coulter (Fullerton, CA). LIVE/DEAD Fixable Blue Dead Cell Stain Kit from Thermo Fisher Scientific (Boston, MA) was used to detect and exclude dead cells. All the reagents were tested and titrated for optimum concentration before usage.
Rinaldi S., de Armas L., Dominguez-Rodríguez S., Pallikkuth S., Dinh V., Pan L., Gӓrtner K., Pahwa R., Cotugno N., Rojo P., Nastouli E., Klein N., Foster C., De Rossi A., Giaquinto C., Rossi P., Palma P, & Pahwa S. (2021). T cell immune discriminants of HIV reservoir size in a pediatric cohort of perinatally infected individuals. PLoS Pathogens, 17(4), e1009533.
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8

Multiparametric Flow Cytometric Analysis

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The following fluorochrome conjugated anti-human mAb were used for flow cytometry studies: PD-1 BV421, CD40L BV605, Perforin PE-Dazzle 594 and CD8 PerCP from BioLegend (San Diego, CA); CD3 BUV496, CD4 APC-Cy7, CD69 BV650, IL-2 BV711, CXCR5 Alexa647, IFN-γ PE-Cy7, TNF-α FITC, Granzyme-B and CD27 BV480 from BD Bioscience (San Jose, CA); IL-21 PE from e-Biosciences, (San Diego, CA); and CD45RO-PE-Cy5.5 from Beckman Coulter (Fullerton, CA). Live/Dead® Fixable Blue Dead Cell Stain Kit from ThermoFisher (Boston, MA) was used to detect and exclude dead cells. All the reagents were tested and titrated before usage.
Rinaldi S., Pallikkuth S., Cameron M., de Armas L.R., Cotugno N., Pahwa R., Richardson B., Saini S.R., Rocca S., Lain M.G., Williams S.L., Palma P, & Pahwa S. (2019). Impact of early ART initiation on HIV-specific CD4 and CD8 T cell function in perinatally infected children. Journal of immunology (Baltimore, Md. : 1950), 204(3), 540-549.
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