Fitc lectin

Manufactured by Merck Group

Sourced in United States

FITC-lectin is a fluorescently labeled lectin, which is a type of protein that binds to specific carbohydrate structures on the surface of cells. The FITC (fluorescein isothiocyanate) dye is used to label the lectin, allowing for the visualization and detection of the bound lectin using fluorescence microscopy or flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

Dil ac ldl, by Thermo Fisher Scientific (3 mentions) Fitc phalloidin, by Merck Group (2 mentions) Pluronic f 127, by Thermo Fisher Scientific (2 mentions) Confocal laser scanning microscope, by Zeiss (1 mentions) Oct compound, by Sakura Finetek (1 mentions) Type 1 collagen, by BD (1 mentions) Tritc lectin, by Merck Group (1 mentions) Lycopersicon esculentum, by Merck Group (1 mentions) 20x objective, by Nikon (1 mentions) Pecam 1, by Santa Cruz Biotechnology (1 mentions) A1rmp multiphoton confocal microscope, by Nikon (1 mentions) Fitc dextran, by Merck Group (1 mentions) Cd133, by Abcam (1 mentions) Dii ac ldl, by Thermo Fisher Scientific (1 mentions) Inverted fluorescence microscope, by Olympus (1 mentions) Plan apochromat 63x 1.40 oil, by Zeiss (1 mentions) Sepharose cl 4b, by Merck Group (1 mentions) Brij 78, by Merck Group (1 mentions) 1 2 dipalmitoyl sn glycero 3 phosphatidyl choline dppc, by Avanti Polar Lipids (1 mentions) Dm6000b, by Leica camera (1 mentions)

26 protocols using fitc lectin

Visualizing Vascular Leakage and Perfusion in Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols

To assess vascular leakage, mice were intravenously injected with TRITC-Dextran (40 kDa, 10 mg/kg, GlycoSci, China). After 1 h, FITC-Lectin (1 mg/kg, Sigma, USA) was injected to analyze tumor vessel perfusion. After 10 min, animals were sacrificed and the tumor tissues were harvested, embedded in optimal cutting temperature compound medium, and cut into cryosections. Sections were fixed in 4% paraformaldehyde, stained with DAPI. Finally, images were acquired from five non-repeating fields of view using CLSM.

Li C., Xiao C., Zhan L., Zhang Z., Xing J., Zhai J., Zhou Z., Tan G., Piao J., Zhou Y., Qi S., Wang Z., Yu P, & Ning C. (2022). Wireless electrical stimulation at the nanoscale interface induces tumor vascular normalization. Bioactive Materials, 18, 399-408.

+ Open protocol

+ Expand

Pharmacodynamics and Efficacy of Cellax-DTX in Pancreatic Xenografts

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pharmacodynamic study (1 dose) –Five mice per treatment group were treated intravenously with saline, Nab-PTX (50mg PTX/kg), or Cellax-DTX (170mg DTX/kg), and were sacrificed at 1, 3 and 6 d post treatment. At each timepoint, tumors were fixed in buffered formalin for histological analysis. A subset of mice (n=5) in this experiment were treated with Cellax-DTX-DiI (fluorescent particles), and were sacrificed 24h after treatment: tumors were frozen in OCT for cryosectioning and histology analysis.
Efficacy study (3 dose): primary human pancreatic xenografts (OCIP19 and OCIP23) were divided into fragments and sutured to the surface of the pancreas in male SCID mice [24 (link)]. When tumors were palpable, mice were treated intravenously (iv) with saline, Nab-PTX (50mg PTX/kg), or Cellax-DTX (170mg DTX/kg) q1w × 3. Weight and mouse health was monitored throughout the course of therapy. Two weeks following the 3^rd treatment, mice were injected with FITC-lectin (Sigma Aldrich, Oakville, ON, CA L0401, 0.05mg in saline), and sacrificed 4h later. On necropsy, tumors were weighed and fixed for histology analysis, and mice were examined for metastatic presentation in the peritoneum, diaphragm, liver, hepatic portal, spleen, and mesentarium.

Ernsting M.J., Hoang B., Lohse I., Undzys E., Cao P., Do T., Gill B., Pintilie M., Hedley D, & Li S.D. (2015). Targeting of metastasis-promoting tumor-associated fibroblasts and modulation of pancreatic tumor-associated stroma with a carboxymethylcellulose-docetaxel nanoparticle. Journal of controlled release : official journal of the Controlled Release Society, 206, 122-130.

+ Open protocol

+ Expand

Vascular Perfusion Labeling and Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

100 μl FITC–lectin (Sigma, St. Louis, MO, USA) at a concentration of 1 mg/mL was injected into the mice via the caudal vein to label the perfused vessels [39 (link)]. Ten minutes after injection, the heart samples were harvested to prepare frozen sections and immunostained with CD31. The vascular perfusion was determined by the ratio of FITC–labeled vessels to CD31-stained vessels.

Liu M., Li S., Yin M., Li Y., Chen J., Chen Y., Zhou Y., Li Q., Xu F., Dai C., Xia Y., Chen A., Lu D., Chen Z., Qian J, & Ge J. (2024). Pinacidil ameliorates cardiac microvascular ischemia–reperfusion injury by inhibiting chaperone-mediated autophagy of calreticulin. Basic Research in Cardiology, 119(1), 113-131.

+ Open protocol

+ Expand

Visualizing Vascular Networks ex vivo

Check if the same lab product or an alternative is used in the 5 most similar protocols

All ex vivo studies followed the same labeling protocol to visualize the vasculature before conducting the experiment. Vascular networks were visualized by labeling with BSI-lectin conjugated to FITC (FITC-lectin; Sigma-Aldrich; St. Louis, MO). BSI-lectin labeling revealed blood and lymphatic vessel networks and vessel type was determined based on morphology. Each well was supplemented with FITC-lectin (1:40) and incubated for thirty minutes under standard conditions followed by two washes with lectin-free media. Arteriole (10–43 μm) and venule (12–50 μm) pairs feeding into the tissue from the border were identified within the vasculature for imaging.

Motherwell J.M., Azimi M.S., Spicer K., Alves N.G., Hodges N.A., Breslin J.W., Katakam P.V, & Murfee W.L. (2017). Evaluation of Arteriolar Smooth Muscle Cell Function in an Ex Vivo Microvascular Network Model. Scientific Reports, 7, 2195.

+ Open protocol

+ Expand

Tumor Vasculature Imaging and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The process for H22 tumor modeling and drug treatment was the same as that followed for the tumor inhibition experiment. Tumor tissues of the PBS, DC101, and CA4-NP + DC101 groups were collected and subjected to FITC-lectin (FITC-labeled tomato lectin) perfusion assays on day 10 (n = 3). Mice were administered an i.v. injection of 10 mg/kg FITC-lectin (Sigma-Aldrich) in PBS (200 μL). After 10 min, tumors were excised, embedded in the OCT compound, and cut into 5-μm-thick sections. Slices were blocked with 10% goat serum at 37 °C for 40 min and incubated with APC-labeled anti-mouse CD31 antibody (1:100) (Thermo Fisher Scientific) at 4 °C overnight. Nuclei were stained with DAPI. Images of the tumors were obtained using a confocal laser scanning microscope (Carl Zeiss, Germany).

Bao X., Shen N., Lou Y., Yu H., Wang Y., Liu L., Tang Z, & Chen X. (2021). Enhanced anti-PD-1 therapy in hepatocellular carcinoma by tumor vascular disruption and normalization dependent on combretastatin A4 nanoparticles and DC101. Theranostics, 11(12), 5955-5969.

+ Open protocol

+ Expand

Angiogenesis Assay Using Matrigel

Check if the same lab product or an alternative is used in the 5 most similar protocols

All animals were maintained, and animal experiments were done in SPF Laboratory Animal Center at Dalian Medical University. The animals used in this research were 6 weeks old male C57/BL6 mice (three mice per group). Matrigel (0.5 mL/plug) containing 250 ng VEGF and 80 units heparin with various concentrations of CS-6 (1 or 5 μM) were injected (S.C.) into near the axillary fossa of mice. Matrigel mixed with medium alone was used as a negative control. After 9 days of implantation, the matrigel plugs were removed and the surrounding tissues were trimmed. The matrigel plugs were embedded with O.C.T. Compound (Sakura Finetek USA, Inc.). Ten-micron sections were stained by FITC-Lectin (Sigma) staining. The number of blood vessels in high power field (HPF, magnification) was counted under immunofluorescent microscope. The results were the means calculated from five replicates of each experiment.

Tang N., Shi L., Yu Z., Dong P., Wang C., Huo X., Zhang B., Huang S., Deng S., Liu K., Ma T., Wang X., Wu L, & Ma X.C. (2015). Gamabufotalin, a major derivative of bufadienolide, inhibits VEGF-induced angiogenesis by suppressing VEGFR-2 signaling pathway. Oncotarget, 7(3), 3533-3547.

+ Open protocol

+ Expand

Aortic Endothelial Sprouting Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Aorta from C57/BL6 mice were removed, cleaned and approximate 1mm aortic rings were embedded in a collagen gel (collagen type I, BD Biosciences) in a 48 well plate, After gel polymerization, DMEM/F12 medium (HyClone) supplemented with 2.5% mouse serum. Additional VEGF (50 ng/mL) and CS-6 (500 nM and 1000 nM) were added and the endothelial sprouts were allowed to develop over 9 days. Thereafter, the samples were fixed (4% paraformaldehyde) and endothelial cells were visualized using FITC-Lectin (Sigma) staining under immunofluorescent microscope. The results were the means calculated from five replicates of each experiment.

+ Open protocol

+ Expand

Fluorescent Staining of Cellular Components

Check if the same lab product or an alternative is used in the 5 most similar protocols

Alexa Fluor 488–Phalloidin (Molecular Probes, Life Technology, Eugene, OR) was used for F-actin staining in fixed cells (Chang et al., 1996 (link)). 4′,6-Diamidino-2-phenylindole (DAPI) was added at a concentration of 1 μg/ml for nucleus staining. Cell wall was stained with FITC-lectin (Sigma-Aldrich, St. Louis, MO; Figure 2) or tetramethylrhodamine isothiocyanate–conjugated lectin (TRITC-lectin; Sigma-Aldrich; Figure 3).

Zhou Z., Munteanu E.L., He J., Ursell T., Bathe M., Huang K.C, & Chang F. (2015). The contractile ring coordinates curvature-dependent septum assembly during fission yeast cytokinesis. Molecular Biology of the Cell, 26(1), 78-90.

+ Open protocol

+ Expand

Immunofluorescent Staining and Quantification of Endothelial-Mesenchymal Transition

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunofluorescent staining was performed for detection of the endothelial marker PECAM-1 (Santa Cruz, Dallas, TX) and the mesenchymal marker monoclonal anti-actin α-smooth muscle actin (α-SMA; Sigma Aldrich, St. Louis, MO). Whole hearts cross-sections were also stained for the transcription factor SLUG/SNAIL (Santa Cruz, Dallas, TX). EndMT was defined as cells staining positive for both PECAM-1 and α-SMA, or EC nuclei staining positive for SLUG/SNAIL, respectively.
In order to label endothelial cells within the myocardium, FITC Lectin (Sigma Aldrich, St. Louis, MO) from Lycopersicon esculentum (tomato) was perfused through the aortic root into the coronaries at a concentration of 20ul/ml at the end of the 3 h perfusion period, which labels endothelial cells as we have previously reported in more detail.^{14 (link)} All slides were visualized using a Zeiss Observer.Z1 fluorescent microscope with a Nikon 20x objective (NA = ×20/0.45). Ten randomly selected fields from each slide were taken and quantification was performed with ImageJ (version 2.0.0-rc-43, obtained from the National Institute of Health, Bethesda, MD).

Vorisek C., Weixler V., Dominguez M., Axt-Fliedner R., Hammer P.E., Lin R.Z., Melero-Martin J.M., del Nido P.J, & Friehs I. (2021). Mechanical strain triggers endothelial-to-mesenchymal transition of the endocardium in the immature heart. Pediatric research, 92(3), 721-728.

+ Open protocol

+ Expand

Longitudinal Vascular Network Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

Vascular networks were imaged longitudinally by labeling with BSI-lectin conjugated to FITC (FITC-lectin; Sigma-Aldrich; St. Louis, MO, USA). BSI-lectin labeled both blood and lymphatic vessel networks. Each well was supplemented with FITC-lectin (1:40) and incubated for 30 min under standard conditions followed by two washes with lectin-free media. Islets were identified by positive DiI staining for imaging.

Dolan R., Lampejo A., Santini-González J., Hodges N.A., Phelps E.A, & Murfee W.L. (2022). A Novel Ex Vivo Method for Investigating Vascularization of Transplanted Islets. Journal of vascular research, 59(4), 229-238.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!