Overnight cultures of Salmonella in LB were diluted 1/1000 in LB or LB without NaCl supplemented with the appropriate antibiotics. Cells were grown in clear-bottomed 96-well black plates (Corning) using a SpectraMax Plus Microplate Reader (Molecular Devices) for agitation. Green fluorescence was measured using a Synergy H1 plate reader (BioTek) with 485-nm excitation and 535-nm emission, and the absorbance in each well was measured at 600 nm.
Clear bottomed 96 well black plates
Manufactured by Corning
Clear-bottomed 96-well black plates are a type of lab equipment designed for various analytical and assay applications. These plates feature a clear bottom and black walls, providing optimal optical properties for applications that require light-based detection or imaging. The clear bottom allows for the transmission of light, while the black walls help to minimize cross-talk between wells and improve signal-to-noise ratio.
Automatically generated - may contain errors
3 protocols using clear bottomed 96 well black plates
1
Salmonella Growth Kinetics Assay
2
Fluorescence-based Reporter Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fluorescence derived from reporter constructs was measured into clear-bottomed 96-well black plates (Corning) using a Synergy H1 plate reader (BioTek). Green fluorescence was measured with excitation 485 nm and emission 535 nm, and the absorbance in each well was measured at 600 nm. Detailed sample extraction and Pi and protein measurements are described in the Supplemental Material .
3
Quantification of Salmonella GFP Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Salmonella cells expressing plasmid-borne gfp fusions were streaked onto either LB or on LB without NaCl agar plates supplemented with the appropriate antibiotics. Following 24 h of growth, fluorescence was visualized with a dark blue light transilluminator and an amber screen. For fluorescence quantification, isolated colonies were resuspended in 1 ml of phosphate-buffered saline (PBS) and 150 μl were aliquoted in a clear-bottomed 96-well black plates (Corning). Green fluorescence was measured using an Infinite M1000 plate reader (Tecan) with 485-nm excitation and 535-nm emission, and the absorbance was measured at 600 nm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!