Anti kim 1 af1817

Manufactured by R&D Systems

Sourced in China

Anti-KIM-1 (AF1817) is a product offered by R&D Systems for research use. It is an antibody that can be used to detect the Kidney Injury Molecule-1 (KIM-1) protein. KIM-1 is a type 1 transmembrane glycoprotein that is expressed in the kidneys and serves as a marker for kidney injury.

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Lab products found in correlation

Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Ab7030, by Abcam (1 mentions) Ab96777, by Abcam (1 mentions) Ab28146, by Abcam (1 mentions) Ab12169, by Abcam (1 mentions) Ab62352, by Abcam (1 mentions) Ab7028, by Abcam (1 mentions) Anti β actin a5441, by Merck Group (1 mentions) Enhanced chemiluminescence kit, by Thermo Fisher Scientific (1 mentions) Benzonase nuclease, by Merck Group (1 mentions) Gapdh 2118, by Cell Signaling Technology (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Anti p53, by ABclonal (1 mentions) Anti caspase 3, by ABclonal (1 mentions)

3 protocols using anti kim 1 af1817

Romidepsin and Lipopolysaccharide Interactions

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Romidepsin (FK228) (S3020), was purchased from Shanghai Selleck Chemicals Co., Ltd. (Shanghai, China). Lipopolysaccharide (LPS) derived from Escherichia coli 055: B5 (L2637) was purchased from Sigma-Aldrich (St Louis, MO, USA). Antibodies to acetyl-histone H3 (4499) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Anti-KIM-1 (AF1817) was purchased from R&D Systems (Chengdu, China). Antibodies to CYP2E1 (ab28146), HDAC1 (ab7028), HDAC2 (ab12169), HDAC3 (ab7030), hepatocyte nuclear factor-1 alpha (HNF-1α) (ab96777) and Nrf2 (ab62352) were purchased from Abcam (Cambridge, UK). Anti-GAPDH (TA-08) was purchased from ZSGB Biotechnology (Beijing, China).

Cheng S., Wu T., Li Y., Huang J, & Cai T. (2020). Romidepsin (FK228) in a Mouse Model of Lipopolysaccharide-Induced Acute Kidney Injury is Associated with Down-Regulation of the CYP2E1 Gene. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 26, e918528-1-e918528-9.

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Immunoblot Analysis of Kidney Protein Markers

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Samples of the kidney cortex and outer medulla were collected from the C57BL/6 and FGF2 mice and lysed in 2% sodium dodecyl sulfate (SDS) buffer with a protease inhibitor cocktail (Sigma) and Benzonase nuclease (EMD Millipore). The kidney lysate was used for immunoblot analysis. Protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Equal amounts of protein were loaded into each lane for SDS-polyacrylamide gel electrophoresis. After being transferred onto polyvinylidene difluoride membranes, the blots were incubated sequentially with a blocking buffer, primary antibodies at 4 °C overnight, and secondaryantibodies for 1 hour at room temperature. Antigens on the blots were revealed using an enhanced chemiluminescence kit (Thermo Fisher Scientific). Primary antibodies were obtained from the following sources: anti-FGF2 (ab106245), anti-Vcam1 (ab134047), antie–α-SMA (ab5694), antifibronectin (ab2413), and anti–cyclophilin B (ab16045) from Abcam; anti-FGF2 (05–118) from Millipore; anti-Kim1 (AF1817) from R&D System; anti–collagen I (NBP1–30054) from Novus Biologicals; GAPDH (#2118) and antivimentin (#3932) from Cell Signaling; and anti–β-actin (A5441) from Sigma. Secondary antibodies for immunoblot analysis were obtained from Jackson ImmunoResearch Laboratories.

Chen Q, & Kadner R.J. (2000). Effect of Altered Spacing between uhpT Promoter Elements on Transcription Activation. Journal of Bacteriology, 182(16), 4430-4436.

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Immunohistochemical Analysis of Mouse Kidney

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Paraffin-embedded mouse kidney sections (3 µm thickness) were prepared by routine procedures. The sections were stained with periodic acid-Schiff (PAS) reagents by standard protocol. For immunohistochemical staining, kidney sections were deparaffinized with xylene, and then gradually rehydrated in ethanol. Hydrogen peroxide (3%) was used to eliminate endogenous peroxidase. Antigen retrieval was performed by microwave using the heat mediated antigen retrieval technique. After blocking with 1% donkey serum for 1 h, the sections were incubated with primary antibodies overnight at 4°C and subsequently washed and incubated with secondary antibodies for 1 h at room temperature. The sections were visualized by using AEC substrate Kit under an Olympus light microscope. The primary antibodies were as follows: anti-Coronavirus nucleocapsid (sc-66012; Santa Cruze Biotechnology), anti-KIM-1 (AF1817; R&D Systems), anti-p53 (A0263; Abclonal), anti-caspase-3 (A2156; Abclonal).

Xu J., Lin E., Hong X., Li L., Gu J., Zhao J, & Liu Y. (2024). Klotho-derived peptide KP1 ameliorates SARS-CoV-2-associated acute kidney injury. Frontiers in Pharmacology, 14, 1333389.

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