C4–2 and C4–2b cells were obtained from ATCC. Cells were cultured in RPMI 1640 medium, GlutaMAX (Gibco-61870), and supplemented with 10% FBS (Sigma-Aldrich), 100 U/mL penicillin, and 100 μg/mL streptomycin. All cells were maintained at 37°C and 5% CO2 and tested for Mycoplasma using MycoAlert. For all studies, passage number was kept below 25. In some cases, cells were treated with BI9774 (a kind gift from Dr. Jon Read, AstraZeneca) or etomoxir (Cayman Chemical). Specific conditions and concentrations are indicated in individual figure legends. For overexpression of PGC1α, pcDNA4mycPGC-1α 50 (link) (Adgene #10974) was transiently transfected into C4–2 cells using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions. As a control, pLPC-N-myc (Adgene#1254) was transfected using the same conditions.
Bi9774
Manufactured by AstraZeneca
BI9774 is a laboratory instrument designed for pharmaceutical research and development. It is used to measure the activity and function of specific biomolecules or cellular processes. The core function of BI9774 is to provide accurate and reliable data for researchers working in the field of drug discovery and development.
Automatically generated - may contain errors
2 protocols using bi9774
1
PGC1α Overexpression in Prostate Cancer
2
PGC1α Overexpression in Prostate Cancer
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C4–2 and C4–2b cells were obtained from ATCC. Cells were cultured in RPMI 1640 medium, GlutaMAX (Gibco-61870), and supplemented with 10% FBS (Sigma-Aldrich), 100 U/mL penicillin, and 100 μg/mL streptomycin. All cells were maintained at 37°C and 5% CO2 and tested for Mycoplasma using MycoAlert. For all studies, passage number was kept below 25. In some cases, cells were treated with BI9774 (a kind gift from Dr. Jon Read, AstraZeneca) or etomoxir (Cayman Chemical). Specific conditions and concentrations are indicated in individual figure legends. For overexpression of PGC1α, pcDNA4mycPGC-1α 50 (link) (Adgene #10974) was transiently transfected into C4–2 cells using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions. As a control, pLPC-N-myc (Adgene#1254) was transfected using the same conditions.
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