Melting points were measured with a Thermo Scientific P12144Q Fisher-Johns Melting Point apparatus. Column chromatography (CC) was conducted with silica gel (EMD Chemicals Inc. brand, Sigma Aldrich). Thin-Layer Chromatography (TLC) was carried out on 0.2 mm thick silica gel plates (Merck 60 F254). Solvent systems used in TLC were: CHCl3/MeOH, Hex/EtOAc, CHCl3/Acetone, CHCl3/MeOH/CH3COOH. The chemicals and reagents were acquired from Sigma-Aldrich (St. Louis, Missouri, USA) or Baker (Baker, Phillipsburg, New Jersey, USA). Spots were detected by TLC using UV lamp (Spectroline brand) and solutions of ceric sulfate in sulfuric acid with subsequent heating. IR spectra were recorded with a Bruker ALPHA ATR-FTIR spectrometer. NMR spectra were obtained on a Bruker NMR 400 spectrometer operated at 400 MHz (1H) and 100 MHz (13C) using deuterated solvents with tetramethylsilane (TMS) as internal reference.
Uv lamp
Manufactured by Spectroline
Sourced in United States
The UV lamp is a device that emits ultraviolet (UV) radiation. It is designed to produce UV light, which is a type of electromagnetic radiation with wavelengths shorter than those of visible light but longer than X-rays. The UV lamp is a core component in various laboratory and industrial applications that require the use of UV light.
Automatically generated - may contain errors
Lab products found in correlation
Alpha atr ftir spectrometer, by Bruker (1 mentions)
1 3 dicqa, by Phytolab (1 mentions)
1 5 dicqa, by Phytolab (1 mentions)
3 4 dicqa, by Phytolab (1 mentions)
3 5 dicqa, by Phytolab (1 mentions)
4 5 dicqa, by Phytolab (1 mentions)
Noa81, by Thorlabs (1 mentions)
24 well plate, by Corning (1 mentions)
4 protocols using uv lamp
1
Characterization of Organic Compounds
2
Photochemical Isomerization of Dicaffeoylquinic Acids
3
Optical Adhesive Fluorescence Microscopy
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UV curable optical adhesive (NOA 81, Thorlabs) was mixed with SYTO 62 (0.5 mM), 10 μl was dispensed on the slide glass, and covered with a #1.5 coverslip. The sample was then cured with exposure to a UV lamp (365 nm, 5 mW/cm2; Spectroline) for 1 min. We targeted ~10 μm depth below the coverslip and photoconverted a single plane (50 mW, 1 min) with a water immersion objective lens (40×, NA 0.8). After photoconversion, the power was lowered to 10 mW and z-stack images were acquired from the surface to the 17 μm depth with 0.85 μm axial spacing (2 s integration for each image). The full-width-half-maximum (FWHM) of the photoconverted signal (green) along z axis was measured and deconvoluted with imaging resolution.
4
UV-Cured Functionalized Carbon Hydrogels
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Either MA- or 4VBC-functionalized AC products were dissolved at a fixed concentration of 0.8 wt.% in either 10 mM HCl (pH 2.1), 17.4 mM acetic acid (AcOH, pH 3.4) or 10 mM phosphate buffered saline (PBS, pH 7.5) solutions supplemented with 1 wt.% I2959 photo-initiator. Resulting AC solutions (0.8 wt.% functionalized AC, 0.992 wt.% I2959) were centrifuged at 3000 rpm for 5 min to remove any air bubble and then cast onto a 24 well plate (Corning Costar, 0.8 g per well). Well plates were irradiated with a UV lamp (346 nm, 8 mW cm−2, Spectroline) for 30 min on both top and bottom side, leading to the formation of hydrogels. Irradiation intensities were measured with an International Light IL1400A radiometer equipped with a broadband silicon detector (model SEL033), a 10 × attenuation neutral density filter (model QNDS1), and a quartz diffuser (model W). The UV-cured hydrogels were carefully removed from the plate and washed in distilled water (15 min, × 3), followed by dehydration via an ascending series of ethanol and air drying.
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