All reagents utilized in this assay were of analytical quality. Sodium chloride (NaCl, 99.5%), and calcium chloride (CaCl2, >99.0%) were obtained from Panreac (Spain, Barcelona). Ultrapure water was supplied from a Milli-Q® system (Millipore); the 3-octanol used as internal standard and other VOCs for identification, namely, 1-butanol, 1-heptanol, 1-octanol, 1-propanol, (E)-2-hexenal, 2-methyl-butanal, 2-ethyl-1-hexanol, 2-methyl-1-propanol, 2-phenylethanol, acetaldehyde, benzaldehyde, ethanol, ethyl acetate, ethyl butanoate, ethyl decanoate, ethyl hexanoate, ethyl octanoate, ethyl propanoate, ethyl 3-hydroxybutanoate, hexyl acetate, and pentanal with purity up to 98% were acquired from Sigma Aldrich (Madrid, Spain). Helium gas purity of 5.0 (Air Liquide, Portugal) was utilized as the GC carrier gas. Solid-phase microextraction holder and divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber were supplied from Supelco (Bellefonte, PA, USA). The Kovats index (KI) was calculated by the injection of a series of C8 to C20 straight-chain n-alkanes (at 40 mg L−1 in n-hexane) produced by Fluka (Buchs, Switzerland).
Hexyl acetate
Manufactured by Merck Group
Sourced in United States, Germany, China
Hexyl acetate is a colorless, volatile liquid chemical compound with a fruity, floral aroma. It is used as a solvent and as a flavoring agent in various industries.
Automatically generated - may contain errors
Lab products found in correlation
Ethyl hexanoate, by Merck Group (7 mentions)
E 2 hexenal, by Merck Group (6 mentions)
Ethyl octanoate, by Merck Group (6 mentions)
1 hexanol, by Merck Group (6 mentions)
Linalool, by Merck Group (6 mentions)
Benzaldehyde, by Merck Group (5 mentions)
Ethyl acetate, by Merck Group (5 mentions)
Hexanal, by Merck Group (5 mentions)
Z 3 hexenyl acetate, by Merck Group (4 mentions)
Decanal, by Merck Group (4 mentions)
α pinene, by Merck Group (4 mentions)
Limonene, by Merck Group (4 mentions)
Nonanal, by Merck Group (4 mentions)
Hexanoic acid, by Merck Group (4 mentions)
Isoamyl acetate, by Merck Group (4 mentions)
Ethyl decanoate, by Merck Group (3 mentions)
1 octanol, by Merck Group (3 mentions)
2 methyl 1 propanol, by Merck Group (3 mentions)
1 butanol, by Merck Group (3 mentions)
Z 3 hexenol, by Merck Group (3 mentions)
19 protocols using hexyl acetate
1
Volatile Organic Compounds Analysis by HS-SPME-GC-MS
2
Odorants and Agonists for Receptor Studies
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The following odors and agonists were used: pentyl acetate (Sigma-Aldrich, Cat. Nr. 109584, 99%), butyl acetate (Fluka, Cat. Nr. 45860, ≥99.5% (GC), ACS Reagent), methyl hexanoate (Fluka, Cat. Nr. 21599, 99.8%, analytical standard grade), propyl acetate (Sigma-Aldrich, Cat. Nr. 133108, 99%; Fluka, Cat. Nr. 40858, ≥99%, analytical standard grade), 3-methylthio-1-propanol (Sigma-Aldrich, Cat. Nr. 318396, 98%; Sigma-Aldrich, Cat. Nr. W341509, ≥98% synthetic, FG grade), hexyl acetate (Sigma-Aldrich, Cat. Nr. 108154, 99%), isobutyl acetate (Fluka, Cat. Nr. 94823, 99.8%, analytical standard grade), 2-heptanone (Sigma-Aldrich, Cat. Nr. W254401, 98% synthetic, FG grade), 3-octanol (Sigma-Aldrich, Cat. Nr. 218405, 99%), (±)-geosmin (Sigma-Adrich, Cat. Nr. UC18, ≥98% [GC]), VUAA1 (CAS Nr. 525582-84-7) was synthesized by the group “Mass Spectrometry/Proteomics” of the Max Planck Institute for Chemical Ecology (Jena, Germany). Stimuli stocks consisted of 100 mM solutions in dimethyl sulfoxide (DMSO, Sigma-Aldrich, Cat. Nr. D8418); working solutions were prepared fresh diluting 100 mM stocks in SES to the desired concentration just before the start of the experiment. Negative controls consisted of the equivalent maximum volume of DMSO used to prepare odor stimuli solutions diluted in SES.
3
Volatile Compound Identification Protocol
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Hexanal, (E)-2-Hexanal, 1-hexanol, (E)-2-hexenol, (E)-3-hexenol, (Z)-2-hexenol, (Z)-3-hexenol, hexyl acetate, and ethyl hexanoate standards used for identification and quantification were obtained from Sigma (≥99%, St Louis, MO, USA).
4
Volatile Identification and Enzyme Activity
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The chemicals used for volatile identification and enzyme activity, including hexyl acetate, E-2-hexenyl acetate, Z-3- hexenyl acetate, hexanol, E-2-hexenol, Z-3-hexenol, 2-octanol, butyl acetate, geranyl acetate, ethyl hexanoate, ethyl benzoate, MeJA, MeSA, JA, and SA were purchased from Sigma-Aldrich (USA).
5
Analysis of Volatile Organic Compounds
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The following compounds were purchased from Sigma–Aldrich (St. Louis, MO, USA): hexanal (98% chemical purity), (E)-2-hexenal (98% chemical purity), octanal (99% chemical purity), nonanal (95% chemical purity), decanal (≥98% chemical purity), (E)-2-nonenal (97% chemical purity), benzaldehyde (≥99% chemical purity), 6-methyl-5-hepten-2-one (99% chemical purity), 1-hexanol (≥99% chemical purity), (Z)-3-hexen-1-ol (98% chemical purity), 1-octen-3-ol (98% chemical purity), propyl 3-methylbutanoate (≥98% chemical purity), 3-methylbutyl propionate (≥98% chemical purity), 3-methylbutyl 3-methylbutanoate (≥98% chemical purity), hexyl acetate (99% chemical purity), ethyl octanoate (≥99% chemical purity), (Z)-3-hexenyl acetate, (Z)-3-hexenyl 3-methylbutanoate (≥97% chemical purity), α-pinene (98% chemical purity), α-phellandrene (≥95% chemical purity), limonene (≥98% chemical purity), (E)-β-ocimene (≥90% chemical purity), p-cymene (99% chemical purity), linalool (97% chemical purity), α-terpinyl acetate, 2-phenylethyl acetate (99% chemical purity), (E)-geranyl acetone (96% chemical purity), hexane (≥99% chemical purity). Ethyl hexanoate (99% chemical purity), 3-methylbutyl acetate (98% chemical purity) were purchased from Alfa Aesar (Ward Hill, MA, USA), δ-3-cerene from Carl Roth (Karlsruhe, Germany), α-farnesene from Bedoukian Research Inc. (Danbury, CT, USA).
6
Analytical Reagents for Chemical Protocols
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Unless otherwise stated, all the reagents used in this study were analytically pure. The water used for various experiments was purified with a Milli-Q purification system. Tween 80, glucose anhydrous, cupric sulfate, potassium sodium tartrate tetrahydrate, sodium hydroxide, magnesium chloride, acetyl-CoA, linoleic acid sodium salt, nicotinamide adenine dinucleotide, dithiothreitol, Triton X-100, 4-morpholineethanesulfonic acid, polyvinylpolypyrrolidone (PVPP), dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium acetate, acetaldehyde, and butanol were obtained from Yuanye Co., Ltd. (Shanghai, China). BTH, E-2-hexenal, hexanal, Z-3-hexenal, E-2-hexenol, Z-3-hexenol, hexanol, Z-3-hexenyl acetate, hexyl acetate, 2-octanol, linolenic acid, and linoleic acid were HPLC grade and obtained from Sigma-Aldrich (Shanghai, China).
7
Volatile Organic Compounds Characterization
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All the pure standards of volatile organic compounds (VOC) used in this manuscript were bought from Sigma-Aldrich (Steinheim, Germany): ethyl acetate (99.9%), hexanal (98%), 2-methyl-1-propanol (>99.5%), 1-butanol (99.9%), (E)-2-hexanal (98%), 1-pentanol (>99%), hexyl acetate (99%), 1-hexanol (>99.5%), cis-3-hexen-1-ol (98%), ethyl octanoate (>98%), 1-heptanol (>99.5%), linalool (>97%), isobutyric acid (99%), butyric acid (>99%), isovaleric acid (99%), geranyl acetate (>97%), β-citronellol (95%), nerol (98%), phenyl ethyl acetate (98%), β-damascenone (>90%), hexanoic acid (>99%), 2-phenyl ethanol (>99%), and β-ionone (>95%).
8
Aroma Compound Characterization Protocol
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1,4-Cineole (98%), 1-decanol (99.9%), 1-hexanol (99.9%), 1-octanol (≥99%), 2-heptanone (99%), 2-nonanone (97%), 2-undecanone (97%), 3-hexen-1-ol (98%), 5-methyl-2-furfural, benzaldehyde (≥99.5%), camphor (99%), decanal (95%), diethyl succinate (≥99%), ethyl 2-methylbutanoate (99%), ethyl butanoate (99%), ethyl decanoate (≥98%), ethyl heptanoate (≥98%), ethyl hexanoate (99%), ethyl isobutanoate (≥98%), ethyl isovalerate (98%), ethyl nonanoate (≥98%), ethyl octanoate (99%), eucalyptol (99%), furfural (99%), hexyl acetate (98%), isoamyl acetate (≥99%), isoamyl alcohol (98%), limonene (99%), linalool oxide (97%), nonanal (≥95%), octanal (≥98%), phenylacetaldehyde (90%), phenylethyl acetate (99%), phenylethyl alcohol (99%), tartaric acid (≥99.5%), α-pinene (99%)), β-cyclocitral (90%), β-damascenone (≥98%), α-ionone (85%), and β-linalool (80%) were supplied by Sigma-Aldrich (Madrid, Spain). Ethanol (99.9%) was purchased from ERBA Reagents (Val de Reuil, France).
9
Identification and Quantification of Compounds
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The standard compounds employed in this study for the identification and quantification, i.e., ethyl butyrate, isoamyl acetate, ethyl hexanoate, hexyl acetate, ethyl lactate, ethyl octanoate, isoamyl hexanoate, ethyl nonanoate, methyl decanoate, ethyl decanoate, isoamyl octanoate, diethyl succinate, β-phenethyl acetate, isoamyl decanoate, isobutanol, isoamyl alcohol, hexanol, E-3-hexenol, phenylethyl alcohol, linalool, α-terpineol, citronellol, E-nerolidol, hexanoic acid, octanoic acid, and decanoic acid, were supplied by Sigma-Aldrich (Germany). Sodium chloride and 4-methyl-2-pentanol (internal standard) were purchased from Merck (Darmstadt, Germany).
10
Volatile Organic Compound Identification
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Nonanal (97%), decanal (97%), and octanal (98%) were obtained from Fluka Production (Buchs, Switzerland). (Z)-3-hexenyl acetate (97%) and (Z)-3-hexen-1-ol (98%) were bought from Carl Roth (Karlsruhe, Germany). Hexyl acetate (99%), methyl benzoate (99%), 2-decen-1-ol (97%), and 1-octen-3-ol (98%) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). (Z)-β-ocimene (95%) was obtained from BOC Sciences (New York, USA). Compounds for which no standards were available were tentatively identified using the NIST-database42 (link).
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