Artemisinin was purchased from Shanghai Yuanye Biological Technology Co., Ltd. (63968649). RPMI1640 and fetal bovine serum were purchased from Gibco. Propidium iodide (PI) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchase from Sigma. Oligo-dT primers and rTaq premix were purchased from Takara, Otsu, Japan. Reverse transcriptase (M-MLV), RNase inhibitor, Taq polymerase and nuclease-free water were all purchased from Promega, Madison, WI, USA. Anti-bcl-2 (0032R, Bioss), anti-bax (0127R, Bioss), anti-caspase-3 (0081R, Bioss), and anti-β-actin (RG00120, Solarbio life sciences) polyclonal or monoclonal antibodies were purchased from Bioss and Solarbio life sciences. HRP-labeled goat anti-rabbit/mouse IgG was purchased from ZSGB-BIO (Beijing, China). All the plates used in present study were purchased from Nunc. All chemicals used were of analytical grade.
Hrp labeled goat anti rabbit mouse igg
Manufactured by ZSGB-BIO
Sourced in China
HRP-labeled goat anti-rabbit/mouse IgG is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed to detect and bind to primary antibodies raised in rabbit or mouse, allowing for the amplification and visualization of the target signal in various immunoassays.
Automatically generated - may contain errors
Lab products found in correlation
Artemisinin, by Yuanye Bio-Technology (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Nuclease free water, by Promega (1 mentions)
Rtaq premix, by Takara Bio (1 mentions)
Oligo dt primer, by Takara Bio (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Taq polymerase, by Promega (1 mentions)
Anti pstat3 tyr705, by Cell Signaling Technology (1 mentions)
Chemiluminescent hrp substrate, by Merck Group (1 mentions)
Anti cd274, by Abcam (1 mentions)
Anti stat3, by ABclonal (1 mentions)
Pvdf membrane, by Thermo Fisher Scientific (1 mentions)
Anti gapdh, by ZSGB-BIO (1 mentions)
2 protocols using hrp labeled goat anti rabbit mouse igg
1
Artemisinin's Cytotoxic Effect Analysis
2
Comprehensive Western Blotting Assay
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Western blotting was performed rely on relevant protocol. Proteins were transferred onto PVDF membranes (ThermoFisher, USA), and incubated 8 h in the 4°C fridge with the following primary antibodies: Anti‐IKBKE (Cell Signaling, USA, 1:1000), Anti‐CD274 (Abcam, USA, 1:1000), Anti‐pSTAT3 (Tyr705) (Cell Signaling, USA, 1:1000), Anti‐STAT3 (ABclonal, UK, 1:1000), Anti‐GAPDH (ZSGB‐Bio, China, 1:2000). Secondary antibodies: HRP labeled goat anti‐rabbit/mouse IgG (ZSGB‐Bio, China, 1:1000). Chemiluminescent HRP substrate (Millipore, USA) and GBOX system (Syngene Company, UK) were used to detect protein expression.
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