Trio tim 3.0t

All participants (patients and controls) underwent a 3T MRI scan (Siemens TIM Trio 3.0T, Siemens Medical Systems, Erlingan, Germany) that included acquisition of T1-weighted multi-echo magnetisation prepared rapid acquisition gradient echo (MPRAGE) structural images. The MRI analysis methods employed here have been previously described in detail, including cortical thickness processing and spherical registration to align subjects’ cortical surfaces (FreeSurfer stable release V. 6.0.0, http://surfer.nmr.mgh.harvard.edu).23 (link)

Images were acquired on a Siemens Trio Tim 3.0T (Erlangen, Germany) at MRI Research Center in Zhongshan Hospital of Xiamen University, China.
A 3D structural MRI was acquired from each subject using a T1‐weighted MPRAGE sequence: TR/TE = 1,900 ms/2.48 ms, FOV = 25 × 25 cm², NEX = 1, matrix = 512 × 256, and slice thickness = 1.0 mm. A DTI pulse sequence with single shot diffusion‐weighted echo planar imaging (TR/TE = 3,600/95 ms, FOV = 24 × 24 cm², NEX = 2, matrix = 128 × 128, and slice thickness = 3 mm) was applied sequentially in 30 noncollinear directions (b‐value = 1,000 s/mm²) with one scan without diffusion weighting (b = 0 s/mm²). Conventional 2D T1 and T2 images were also acquired for any incidental findings.

An inverted fluorescence microscope (Ti-U, Nikon, JPN) with a CCD camera (Digital Sight DS-Fi2, Nikon, JPN), a stereoscope (SZ760-DM601, Optec, China) and a scanning electron microscope (SEM, JSM-6010LA, JEOL, JPN) were used to characterize the morphology and structure of the eccentric magnetic microcapsules. The samples for SEM were prepared by slicing the eccentric magnetic microcapsules in halves along the vertical plane of the thin wall and then sputter-coating the sections of them with gold. Their average diameters and the standard deviations were calculated by measuring over 50 microcapsules randomly selected from the optical micrographs of each group. SEM was also applied for the elemental analysis of iron distribution in the microcapsules. An optical coherence tomography (OCT) scanner (HSO-2000, Teksqray, China) and an MRI scanner (Trio Tim 3.0T, Siemens, GER) were used to visualize those injected into the phantom agarose hydrogels (Aladdin, China).

Structural data were assessed using a three-dimensional, T1-weighted, magnetization prepared rapid gradient echo sequence (MPRAGE) in Graz and Vienna (Trio Tim 3.0T, manufactured by Siemens Healthcare, Erlangen, Germany) with whole brain coverage. The selected sequence parameters for PRODEM were as follows: Repetition time: 1,900/2,000 ms, inversion time: 900 ms, echo time: 2.19 ms, flip angle: 9° and a resolution of: 1 mm × 1 mm × 1 mm/1.2 mm. For ASPS-Fam, the selected sequence parameters were as follows: Repetition time: 1,900, inversion time: 900 ms, echo time: 2.19 ms, flip angle: 9° an isotropic resolution of 1 mm.