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19 protocols using trio tim 3.0t

1

3T MRI Resting-State Neuroimaging Protocol

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The MRI data were collected using a Siemens 3.0T (TIM-Trio, Siemens Medical Solutions, Erlangen, Germany) MRI scanner with a 32-channel head coil. Foam padding was used to reduce head motion. The scan sequences of each subject included conventional T2WI, 3D-T1WI and resting-state fMRI. Two-dimensional T2-weighted turbo spin echo (TSE) and T1-weighted MPRAGE sequences were used to detect brain lesions. Gradient echo plane imaging (EPI) sequence was used to obtain BOLD image with the following parameters: measurement = 200, echo time (TE) = 30ms, repeat time (TR) = 2500 ms, flip angle (FA) = 90°, field of view (FOV) = 220 mm × 220 mm, matrix = 96 × 96, slice thickness = 3 mm, slice gap = 0.3 mm, and number of slices = 40. The total scan time of resting-state data was 500 s. The subjects were asked to close their eyes, relax physically and mentally, stay awake, keep their heads and bodies still, and try not to think about anything.
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2

Comprehensive Whole-Body MRI Protocol

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All MRI studies were performed on a Siemens 3.0T TIM-Trio MRI scanner (Siemens Healthcare, Malvern, PA, USA) with large 6-channel body matrix phased-array coil and 12-channel spine matrix phased-array coil. The upper and lower body 3D images were acquired separately to cover the whole length of the animal with about 30% overlap between the two image sets. Two to three common fiducial markers were included in each set to assist the PET/MRI co-registration and image merging. The structural T1 images were acquired with a 3D Flash sequence (repetition time/echo time 6.33/1.51 ms, flip angle 10 deg, matrix 512 × 512, coronal, field of view 500 x 250 mm, slice thickness 1.2 mm, 128 slices without gap, 6 averages, accelerate factor 2 GRAPPA, and scan time 366 s).
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3

Multimodal MRI Imaging Protocol

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All 36 participants underwent MR imaging, after providing written consent, following recruitment on a Siemens 3.0T Tim Trio (Siemens Healthineers, Erlangen, Germany) using the system's 12-channel head array coil at the Royal Melbourne Hospital as part of the AIBL Active study 19 and VEL015 study. 20, 21 The MR sequences were as follows:
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4

Multimodal 3T MRI Neuroimaging Protocol

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All participants (patients and controls) underwent a 3T MRI scan (Siemens TIM Trio 3.0T, Siemens Medical Systems, Erlingan, Germany) that included acquisition of T1-weighted multi-echo magnetisation prepared rapid acquisition gradient echo (MPRAGE) structural images. The MRI analysis methods employed here have been previously described in detail, including cortical thickness processing and spherical registration to align subjects’ cortical surfaces (FreeSurfer stable release V. 6.0.0, http://surfer.nmr.mgh.harvard.edu).23 (link)
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5

Structural MRI analysis of cortical thickness

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All participants (patients and controls) underwent a 3T MRI scan (Siemens TIM Trio 3.0T, Siemens Medical Systems, Erlingan, Germany) that included acquisition of T1-weighted multi-echo magnetisation prepared rapid acquisition gradient echo (MPRAGE) structural images. The MRI analysis methods employed here have been previously described in detail, including cortical thickness processing and spherical registration to align subjects’ cortical surfaces (FreeSurfer stable release V. 6.0.0, http://surfer.nmr.mgh.harvard.edu).23 (link)
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6

Structural MRI Analysis of Cortical Thickness

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All participants (patients and controls) underwent a structural MRI scan (Siemens TIM Trio 3.0T; Siemens Medical Systems). The MRI analysis methods used have been previously described in detail, including processing of cortical thickness and spherical registration to align participants’ cortical surfaces (Freesurfer 5.3; http://surfer.nmr.mgh.harvard.edu).10 (link)
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7

Structural and Diffusion MRI Brain Imaging Protocol

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Images were acquired on a Siemens Trio Tim 3.0T (Erlangen, Germany) at MRI Research Center in Zhongshan Hospital of Xiamen University, China.
A 3D structural MRI was acquired from each subject using a T1‐weighted MPRAGE sequence: TR/TE = 1,900 ms/2.48 ms, FOV = 25 × 25 cm2, NEX = 1, matrix = 512 × 256, and slice thickness = 1.0 mm. A DTI pulse sequence with single shot diffusion‐weighted echo planar imaging (TR/TE = 3,600/95 ms, FOV = 24 × 24 cm2, NEX = 2, matrix = 128 × 128, and slice thickness = 3 mm) was applied sequentially in 30 noncollinear directions (b‐value = 1,000 s/mm2) with one scan without diffusion weighting (b = 0 s/mm2). Conventional 2D T1 and T2 images were also acquired for any incidental findings.
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8

Characterization of Eccentric Magnetic Microcapsules

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An inverted fluorescence microscope (Ti-U, Nikon, JPN) with a CCD camera (Digital Sight DS-Fi2, Nikon, JPN), a stereoscope (SZ760-DM601, Optec, China) and a scanning electron microscope (SEM, JSM-6010LA, JEOL, JPN) were used to characterize the morphology and structure of the eccentric magnetic microcapsules. The samples for SEM were prepared by slicing the eccentric magnetic microcapsules in halves along the vertical plane of the thin wall and then sputter-coating the sections of them with gold. Their average diameters and the standard deviations were calculated by measuring over 50 microcapsules randomly selected from the optical micrographs of each group. SEM was also applied for the elemental analysis of iron distribution in the microcapsules. An optical coherence tomography (OCT) scanner (HSO-2000, Teksqray, China) and an MRI scanner (Trio Tim 3.0T, Siemens, GER) were used to visualize those injected into the phantom agarose hydrogels (Aladdin, China).
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9

Brain Structural Imaging Protocol

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Structural data were assessed using a three-dimensional, T1-weighted, magnetization prepared rapid gradient echo sequence (MPRAGE) in Graz and Vienna (Trio Tim 3.0T, manufactured by Siemens Healthcare, Erlangen, Germany) with whole brain coverage. The selected sequence parameters for PRODEM were as follows: Repetition time: 1,900/2,000 ms, inversion time: 900 ms, echo time: 2.19 ms, flip angle: 9° and a resolution of: 1 mm × 1 mm × 1 mm/1.2 mm. For ASPS-Fam, the selected sequence parameters were as follows: Repetition time: 1,900, inversion time: 900 ms, echo time: 2.19 ms, flip angle: 9° an isotropic resolution of 1 mm.
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10

Comprehensive MRI Examination Protocol

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We used the Siemens Trio TIM 3.0T magnetic resonance imaging system (Germany) and standard head coil. The patient was relaxed naturally in supine position and maintained the forehead and face in horizontal position and the nose root in the central line of the coil. Plain scan in sagittal and axial position T1WI, T2WI, FLAIR sequence, and b value of axial DWI (0, 800 s/mm2) were performed. Enhanced scan in sagittal, axial, and coronal positions T1WI were performed with 0.1 mmol/kg of Gd-DTPA as a contrast medium. Scanning parameters were: T1WI: TR 260.0 ms, TE 2.46 ms; T2WI: TR 3800 ms, TE 93.0 ms; FLAIR: TR 4500 ms, and TE 93.0 ms. DWI scanning was carried out by SE-EPI sequence, with b=0 and 1000 s/mm2, TR 3500 ms, TE 119 ms; 23×23cm field of vision, 5-mm-layer thick, 0.3-mm-layer spacing, and 20 layers.
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