Mitotracker probes deep red and orange

Manufactured by Thermo Fisher Scientific

MitoTracker probes (Deep Red and Orange) are fluorescent dyes used for labeling and tracking mitochondria in live cells. These probes passively diffuse across the plasma membrane and accumulate in active mitochondria, where they exhibit fluorescence. The Deep Red probe emits in the far-red region of the spectrum, while the Orange probe emits in the orange region.

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Lab products found in correlation

Dmem f12, by Thermo Fisher Scientific (2 mentions) Mcf 7 cell, by American Type Culture Collection (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Ab15251, by Abcam (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

MitoTracker Red (756 citations) MitoTracker Deep Red (547 citations) MitoTracker (469 citations) MitoTracker Orange (138 citations) MitoTracker probe (48 citations) MitoTracker Red probe (17 citations) MitoTracker Deep Red probe (4 citations) MitoTracker orange probe (3 citations)

2 protocols using mitotracker probes deep red and orange

Measuring Telomerase Activity in MCF7 Cells

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MCF7 cells were purchased from the ATCC. Gibco-brand cell culture media (DMEM and DMEM/F12) was purchased from Life Technologies. The lentiviral vector encoding the hTERT promoter linked to eGFP was custom-made by Genecopoeia (USA). MitoTracker probes (Deep Red and Orange) were purchased from Molecular Probes/Invitrogen, via Life Technologies. The telomerase inhibitor IX (MST-312; sc-204333) was obtained commercially from Santa Cruz Biotech (USA).

Lamb R., Ozsvari B., Bonuccelli G., Smith D.L., Pestell R.G., Martinez-Outschoorn U.E., Clarke R.B., Sotgia F, & Lisanti M.P. (2015). Dissecting tumor metabolic heterogeneity: Telomerase and large cell size metabolically define a sub-population of stem-like, mitochondrial-rich, cancer cells. Oncotarget, 6(26), 21892-21905.

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Breast Cancer Cell Line Manipulation

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Breast cancer cell lines (MCF7 and T47D) were originally purchased from the ATCC. Gibco-brand cell culture media (DMEM and DMEM/F12) was purchased from Life Technologies. Lentiviral vectors encoding WNT1 [EX-B0110-Lv105(puro)] and FGF3 [EX-A0154-Lv151(neo)] were obtained commercially from Genecopoeia (USA), along with appropriate empty vector controls [EX-Neg-Lv105(puro) and EX-Neg-Lv151(neo)]. Antibodies directed against FGF3 (# HPA012692, Sigma) and WNT1 (# ab15251, Abcam) were also obtained commercially. MitoTracker probes (Deep-Red and Orange) were purchased from Molecular Probes, via Life Technologies.

Lamb R., Bonuccelli G., Ozsvári B., Peiris-Pagès M., Fiorillo M., Smith D.L., Bevilacqua G., Mazzanti C.M., McDonnell L.A., Naccarato A.G., Chiu M., Wynne L., Martinez-Outschoorn U.E., Sotgia F, & Lisanti M.P. (2015). Mitochondrial mass, a new metabolic biomarker for stem-like cancer cells: Understanding WNT/FGF-driven anabolic signaling. Oncotarget, 6(31), 30453-30471.

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