Disodium EDTA, sodium phosphate, o-phosphoric acid (85%, HPLC grade), acetonitrile (Optima LC/MS grade), acetone (HPLC grade), and 2-propanol (99.9 %, HPLC grade) were purchased from Fisher Scientific (Fair Lawn, NJ). Sodium acetate and glacial acetic acid (99.9 %) were from J.T. Baker (Phillipsburg, NJ). Sodium 1-octanesulfonate (SOS), L-ascorbic acid (AA), 3,4 dihydroxyphenylacetic acid (DOPAC), 5-hydroxyindole-3-acetic acid (5-HIAA), homovanillic acid (HVA), dopamine hydrochloride (DA), serotonin hydrochloride (5-HT), 3-methoxytyramine hydrochloride (3-MT) were from Sigma Aldrich (St. Louis, MO). Nomifensine maleate (Sigma) was dissolved in phosphate-buffered saline (PBS: 155 mM NaCl, 100 mM NaH2PO4, pH 7.40) and administered at 20 mg/kg (i.p.). Artificial cerebrospinal fluid (aCSF) was 144 mM NaCl, 2.7 mM KCl, 1.1.75 mM CaCl2, 1 mM MgCl2, 1.75 mM NaH2PO4 and 2.5 mM NaHCO3. All chemicals were used as received without further purification. Ultrapure water was obtained from a Millipore Milli-Q Synthesis A10 system (Bedford, MA).
Milli q synthesis a10 system
Manufactured by Merck Group
Sourced in United States
The Milli-Q Synthesis A10 system is a water purification system designed for laboratory use. It produces ultrapure water by using a multi-step purification process. The core function of the Milli-Q Synthesis A10 system is to provide a consistent supply of high-quality water for various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Sytox green, by Thermo Fisher Scientific (3 mentions)
O phosphoric acid, by Thermo Fisher Scientific (1 mentions)
2 propanol, by Thermo Fisher Scientific (1 mentions)
Optima lc ms grade, by Thermo Fisher Scientific (1 mentions)
Disodium edta, by Thermo Fisher Scientific (1 mentions)
Sodium 1 octanesulfonate, by Merck Group (1 mentions)
3 4 dihydroxyphenylacetic acid, by Merck Group (1 mentions)
L ascorbic acid, by Merck Group (1 mentions)
Diethyl ether, by Kanto Chemical (1 mentions)
Dinch, by BASF (1 mentions)
Diethyl ether, by Fujifilm (1 mentions)
Dehydrated toluene, by Fujifilm (1 mentions)
Methanol, by Fujifilm (1 mentions)
Acetone, by Fujifilm (1 mentions)
1 4 dioxane, by Fujifilm (1 mentions)
Dulbecco s phosphate buffered saline without calcium chloride, by Merck Group (1 mentions)
Glass coverslip, by Matsunami (1 mentions)
3 aminopropyltriethoxysilane aptes, by Shin-Etsu Chemical (1 mentions)
Neomycin, by Merck Group (1 mentions)
Acetone, by Merck Group (1 mentions)
9 protocols using milli q synthesis a10 system
1
HPLC Analysis of Neurotransmitters
2
Synthesis and Characterization of Novel SANSOCIZERs
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Two types of SANSO CIZER (DL9TH, Chemical Abstracts Service [CAS] number 1609185-22-9; and DOTH, CAS number 2915-49-3) were synthesized by New Japan Chemical Co., Ltd. (Osaka, Japan). Epoxidized soybean oil was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). DINCH (CAS number 166412-78-8) was provided by BASF (Ludwigshafen, Germany), while DEHP (CAS number 117-81-7), DEHP-d4, diethyl ether of pesticide residue analysis grade, and phthalate-analytical-grade hexane were purchased from Kanto Chemical Co. (Tokyo, Japan). Sodium chloride from pesticide residue analysis grade, phthalate-analytical-grade anhydrous sodium sulfate (Wako Pure Chemical Industries, Ltd., Tokyo, Japan), and ultrapure water obtained using a Milli-Q Synthesis A10 system (Millipore, Tokyo, Japan) were used to prepare samples for gas chromatography/tandem mass spectroscopy (GC-MS/MS) analysis. Other chemicals were purchased from Wako Pure Chemical Industries. Prior to use, all tools made of glass, metal, or Teflon were heated to 250°C for more than 16 h.
3
Synthesis of Cationic Thermoresponsive Polymers
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N-Isopropylacrylamide (IPAAm) and (3-acrylamidopropyl)trimethylammonium chloride (APTAC) were provided by KJ Chemicals (Tokyo, Japan). IPAAm was recrystallized from hexane, and APTAC was used as received. 3-Aminopropyltriethoxysilane (APTES) was purchased from Shin-Etsu Chemical (Tokyo, Japan). 4-Cyano-4-[(dodecylsulfanylthiocarbonyl)sulfanyl]pentanoic acid (CDPA) as well as Dulbecco’s phosphate buffered saline without calcium chloride and magnesium chloride (DPBS) were obtained from Sigma-Aldrich (St. Louis, Missouri, USA). N-Hydroxysuccinimide (NHS), N,N’-dicyclohexylcarbodiimide (DCC), 4,4ʹ-azobis(4-cyanovaleric acid) (V-501), tris(2-carboxyethyl)phosphine hydrochloride (TCEP), diethyl ether, dehydrated toluene, methanol, acetone, dehydrated dichloromethane (DCM), and 1,4-dioxane were obtained from FUJIFILM Wako Pure Chemicals (Osaka, Japan) and used without further purification. Glass coverslips (24 mm × 50 mm, thickness: 0.2 mm) were purchased from Matsunami Glass (Osaka, Japan). Water used in this study was purified using a Milli-Q Synthesis A10 system (Millipore, Billerica, Massachusetts, USA).
4
Polymeric Carriers and Antimicrobial Compounds: Synthesis and Characterization
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The polymers PMVE/MA-Es (CAS number: 25087-06-3; MW: 130 kg/mol), provided as 50% w/w solution in ethanol, and PMVE/MA-Ac (CAS number: 25153-40-6; MW: 216 kg/mol), in powder format, were purchased from Sigma-Aldrich (St. Louis, MO, USA). Amikacin (Mw: 585.6 g/mol; 250 mg/mL) and cefotaxime (MW: 455.5 g/mol; powder) were acquired from Laboratorios Normon (Tres Cantos, Spain). Neomycin (MW: 614.6 g/mol; 10 mg/mL) was obtained from Sigma-Aldrich and ciprofloxacin (MW: 331.3 g/mol; 2 mg/mL) from Genéricos Españoles laboratorios S.A. (Las Rozas, Spain). The chemical structure inputs of all these compounds are shown in Figure 1 .
Phosphate buffered saline (PBS; pH 7.4), Mueller-Hinton broth (MHB; powder format), methanol, acetone, phosphoric acid and trifluoroacetic acid with HPLC grade purity, and dimethylsulfoxide (DMSO) and ethanol with purity >95%, were all from Sigma-Aldrich. The water used was double distilled and deionized (DDW) from a Milli-Q Synthesis A10 system (Millipore, Madrid, Spain).
Phosphate buffered saline (PBS; pH 7.4), Mueller-Hinton broth (MHB; powder format), methanol, acetone, phosphoric acid and trifluoroacetic acid with HPLC grade purity, and dimethylsulfoxide (DMSO) and ethanol with purity >95%, were all from Sigma-Aldrich. The water used was double distilled and deionized (DDW) from a Milli-Q Synthesis A10 system (Millipore, Madrid, Spain).
5
Characterization of Antimicrobial Peptides
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Levofloxacin (LVFX), N-methylmorpholine (NMM) and resazurin were purchased from Sigma-Aldrich, Co. LLC (St. Louis, MO, USA). Bicinchoninic acid (BCA) and SYTOX Green were purchased from ThermoFisher Scientific, Inc. (Waltham, MA, USA). Fluoro-N,N,N′,N′-tetramethylformamidinium hexafluorophosphate (TFFH) was purchased from EMD Millipore (a division of Merck KGaA; Darmstadt, Germany), Fmoc-Gly-Wang resin from Peptides International, Inc. (Louisville, KY, USA), and DiSC(3)5 from Anaspec, Inc. (Fremont, CA, USA). Deionized water (dH2O) was prepared using a Milli-Q Synthesis A10 system (Millipore). The bacterial strains E. coli (#25922) and B. cereus (#11778) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). Pep-4 (≥95% purity) was custom synthesized by Genscript USA, Inc. (Piscataway, NJ, USA). Melittin (≥95%) was purchased from Anaspec, Inc., and indolicidin was synthesized (≥95% purity) by AAPPTec, LLC (Louisville, KY, USA). Peptide identities were verified via matrix assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry using a Shimadzu AXIMA Performance. Additionally, Pep-4 and the conjugate were submitted for amino acid analysis (UC Davis Genome Center Proteomics Core Facility) in order to establish molar concentrations.
6
Quetiapine Tablet Stability Evaluation
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A single lot of QF 400 mg tablet (Alkem, India) was used to conduct the stability testing. Ora-Blend and Ora-Sweet vehicles used in this study were obtained from Perrigo (Minneapolis, MN). Acetonitrile was purchased from Fisher Scientific Canada. Quetiapine standard, phosphoric acid and methanol was purchased from Sigma-Aldrich (MO, USA). Trimethylamine (TEA) was purchased from Janssen Pharmaceutical (Belgium). Water was purified using a Milli-Q Synthesis A10 system (Millipore, Etobicoke, ON, Canada).
7
Pasteurized Milk and Apple Juice Assays
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Pasteurized skimmed milk and apple juice were purchased from a local market in Madrid (Spain). Both samples were kept refrigerated until its subsequent manipulation for the assays with the ingredient OsLu.
Sodium phosphate buffer (0.1 M, pH 6.8) and sodium citrate buffer (0.05 M, pH 3.4) were prepared with chemicals of analytical grade (Panreac, Barcelona, Spain).
Ultrapure water quality (18.2 MΩ cm) with 1-5 ppb total organic carbon (TOC) and <0.001 EU mL -1 pyrogen levels was produced in-house using a laboratory water purification Milli-Q Synthesis A10 system from Millipore (Billerica, MA, USA).
Sodium phosphate buffer (0.1 M, pH 6.8) and sodium citrate buffer (0.05 M, pH 3.4) were prepared with chemicals of analytical grade (Panreac, Barcelona, Spain).
Ultrapure water quality (18.2 MΩ cm) with 1-5 ppb total organic carbon (TOC) and <0.001 EU mL -1 pyrogen levels was produced in-house using a laboratory water purification Milli-Q Synthesis A10 system from Millipore (Billerica, MA, USA).
8
Analytical Grade Reagents and Ultrapure Water
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All chemicals used were of analytical grade. Hydrochloric acid, acetic acid and potassium chloride were obtained from Panreac (Barcelona, Spain). Sep-Pack cartridges (C 18 ) were purchased from Waters Millipore (Milford, MA, USA). Ultrapure water quality (18.2 MΩ cm) with 1-5 ppb total organic carbon (TOC) and <0.001 EU mL -1 pyrogen levels was produced in-house using a laboratory water purification Milli-Q Synthesis A10 system from Millipore (Billerica, MA, USA).
9
Electrophoresis of RNA with Phenylboronic Acid
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Reagents All of the RNAs and DNAs were custom-synthesized and HPLC-purified by Gene Design Inc. (Osaka, Japan) and Nihon Gene Reserach Laboratoriers Inc. (Sendai, Japan), respectively. Other reagents were of commercially available analytical grade, and were used without further purification. Mouse testis total RNA was purchased from Biochain Institute Inc. (Newark, CA, USA). The concentrations of DNA and RNAs were determined from the absorbance at 260 nm according to the literature. 19 Water was deionized (≥18.0 MΩ cm specific resistance) by an Elix 5 UV Water Purification System and a Milli-Q Synthesis A10 system (Millipore Corp., Bedford, MA), followed by filtration through a BioPak filter (Millipore Corp., Bedford, MA) in order to remove RNase.
As shown in Table 1, we prepared buffer solutions with standard composition for RNA electrophoresis. Considering the strong binding of phenylboronic acid to diol-containing compounds at higher pH, 20 HEPES and MOPS buffers were adjusted to pH 8.2 and 7.9, respectively. Both TAE and TBE buffers were adjusted to pH 8.5.
As shown in Table 1, we prepared buffer solutions with standard composition for RNA electrophoresis. Considering the strong binding of phenylboronic acid to diol-containing compounds at higher pH, 20 HEPES and MOPS buffers were adjusted to pH 8.2 and 7.9, respectively. Both TAE and TBE buffers were adjusted to pH 8.5.
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