Anti hec1

Manufactured by Abcam

Anti-HEC1 is a laboratory reagent used for the detection and quantification of the HEC1 protein. HEC1 (Highly Expressed in Cancer 1) is a component of the Ndc80 complex, which is essential for proper chromosome segregation during cell division. Anti-HEC1 can be used in various research applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to investigate the expression and localization of HEC1 in biological samples.

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Lab products found in correlation

Anti phospho akt ser473, by Cell Signaling Technology (1 mentions) Anti nek3, by Abcam (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Horseradish peroxidase conjugated anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Horseradish peroxidase conjugated anti mouse igg, by Jackson ImmunoResearch (1 mentions) Anti tubulin, by Merck Group (1 mentions) Anti gfp, by MBL Life Science (1 mentions) Protease inhibitor, by Roche (1 mentions)

2 protocols using anti hec1

Antibody Immunoblot Screening Protocol

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For immunoblots, proteins were probed with one of the following primary antibodies in either 5% non-fat milk or 5% bovine serum albumin: anti-HEC1 (#3613, 1:1000 dilution) and anti-NEK3 (#37636, 1:800 dilution) antibodies from Abcam; anti-XPO1 (#46249, 1:1000 dilution), anti-phospho-Akt (Ser473) (#4051, 1:1000 dilution), and anti-GAPDH (#5174, 1:3000 dilution) from Cell Signaling Technology; anti-NUP205 (Santa Cruz Biotechnology, 1:200 dilution), and anti-tubulin (acetylated) (T6793, Sigma-Aldrich, 1:1000 dilution for WB).

Zhang Y., Chen W., Zeng W., Lu Z, & Zhou X. (2020). Biallelic loss of function NEK3 mutations deacetylate α-tubulin and downregulate NUP205 that predispose individuals to cilia-related abnormal cardiac left–right patterning. Cell Death & Disease, 11(11), 1005.

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Immunoblotting Protocol for Centromeric Proteins

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Cells were suspended in lysis buffer (50 mM Na Phosphate pH 8.0, 30 mM NaCl, 0.1% NP40, 5 mM β-mercaptoethanol, protease inhibitors (Roche) and phosphatase inhibitors) and then resuspended in 2 × SDS loading buffer. The following antibodies were used for immunoblotting: anti-GFP (1:2,500, MBL international), anti-Hec1(1:1,500, Abcam), anti-tubulin (1:4,000, Sigma), anti-CENP-T (1:5,000), anti-CENP-C (1:5,000), horseradish peroxidase-conjugated anti-rabbit IgG (1:100,000, Jackson ImmunoResearch) and horseradish peroxidase-conjugated anti-mouse IgG (1:100,000, Jackson ImmunoResearch)13 (link)35 (link).

Suzuki A., Badger B.L, & Salmon E.D. (2015). A quantitative description of Ndc80 complex linkage to human kinetochores. Nature Communications, 6, 8161.

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