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Fpra14

Manufactured by Bio-Techne
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Sourced in United Kingdom

The FPRa14 is a lab equipment product manufactured by Bio-Techne. It is a device designed to perform specific functions in a laboratory setting. However, a detailed and unbiased description of its core function cannot be provided while maintaining conciseness, without making interpretations or extrapolations.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions) Boc mlf, by Bio-Techne (1 mentions) Cyclosporin h, by Merck Group (1 mentions) Doxycycline, by Merck Group (1 mentions) Sb431542, by Bio-Techne (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions)

2 protocols using fpra14

1

Neuroblastoma Cell Lines Characterization

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Mouse and Human Neuroblastoma cells (N2a (ECACC 89121404), IMR-32 (ECACC 86041809), SH-SY5Y (ECACC 94030304)) were obtained from European Collection of Authenticated Cell Cultures (UK). Eagle’s Minimum Essential Medium high glucose (EMEM), Dulbecco’s Modified Eagle’s Medium high glucose (DMEM), all additional media components and cyclosporin H were purchased from Sigma Aldrich (UK). FPRa14, Boc-MLF and WRW4 were obtained from Tocris Bioscience (UK). SilencerTM select siRNA duplexes for mouse Fpr1 (siRNA ID s66215), mouse Fpr2 (siRNA ID 66212) plus negative control no.1 siRNA and Lipofectamine RNAiMAX were purchased from ThermoFisher Scientific (UK). All other chemicals used were of reagent grade.
Cussell P.J., Howe M.S., Illingworth T.A., Gomez Escalada M., Milton N.G, & Paterson A.W. (2019). The formyl peptide receptor agonist FPRa14 induces differentiation of Neuro2a mouse neuroblastoma cells into multiple distinct morphologies which can be specifically inhibited with FPR antagonists and FPR knockdown using siRNA. PLoS ONE, 14(6), e0217815.
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2

Induction of Oncogenic kras Signaling

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20 μg/ml doxycycline (Sigma, D9891) was added from 3 days post-fertilization (dpf) to 8 dpf to induce krasG12V-EGFP expression. Lipopolysaccharides (LPS) (Sigma, L4391), FPR-A14 (Tocris, 2826), PR-39 (Tocris, 1947) and SB431542 (Tocris, 1614) were first dissolved in dimethyl sulfoxide as stocks and used for larva exposure at 5 ng/ml, 2.5 μM, 50 nM and 2.5 μM respectively from 4 to 8 dpf. The dosages were selected based on the highest all-survival concentrations.
Yan C., Huo X., Wang S., Feng Y, & Gong Z. (2015). Stimulation of hepatocarcinogenesis by neutrophils upon induction of oncogenic kras expression in transgenic zebrafish. Journal of Hepatology, 63(2), 420-428.
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