Anti ter119

Manufactured by Thermo Fisher Scientific

Sourced in Germany

Anti-Ter119 is a monoclonal antibody that binds to the Ter119 antigen, which is expressed on the surface of red blood cells. It is commonly used in flow cytometry applications for the identification and enumeration of red blood cells.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Anti-Ter119-PE Anti-mouse Ter119-APC Anti-Ter119 (TER-119) Biotinylated anti-CD8 and Ter119 antibodies Anti-Ter119-biotin Biotin-conjugated anti-Ter119 antibody Anti-TER119-APC Anti-Ter119 (clone TER119; Anti-Ter119-APC-eFluor 780 APC)–conjugated anti-Ter119

13 protocols using anti ter119

Mouse Blood Cell Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole mouse blood was collected into microcentrifuge tubes containing 100μl HEPES medium (132mM NaCl, 6mM KCl, 1mM MgSO4, 20mM HEPES, and 5mM glucose) as previously described². Samples were diluted 2× in HEPES medium, and then centrifuged at RT for 15 min at 100 × g. Platelet rich plasma was incubated for 15 min at RT in 100μl of HEPES medium containing the following antibodies: Anti-CD45.2, anti-CD31, anti-CD9, anti-GARP and anti-Ter119 (eBioscience) prior to flow cytometry analysis. Data were acquired using BD Fortessa flow cytometers and analysed using FlowJo (TreeStar).

Nasrallah R., Imianowski C.J., Bossini-Castillo L., Grant F.M., Dogan M., Placek L., Kozhaya L., Kuo P., Sadiyah F., Whiteside S.K., Mumbach M.R., Glinos D., Vardaka P., Whyte C.E., Lozano T., Fujita T., Fujii H., Liston A., Andrews S., Cozzani A., Yang J., Mitra S., Lugli E., Chang H.Y., Unutmaz D., Trynka G, & Roychoudhuri R. (2020). A distal enhancer at risk locus 11q13.5 promotes suppression of colitis by Treg cells. Nature, 583(7816), 447-452.

+ Open protocol

+ Expand

Quantification of Hematopoietic Stem and Progenitor Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

BMCs (2×10⁶) were blocked with Fc-block for 10 min, followed by staining with biotin-conjugated anti-CD3e (eBioscience), anti-CD45R/B220 (eBioscience), anti-Gr-1 (eBioscience), anti-CD11b (eBioscience), and anti-Ter-119 (eBioscience) antibodies for 30 min. Cells were then washed once and stained for 30 min with antibodies (1∶100 dilution) to c-Kit (CD117 2B8, fluorescein APC-conjugated; BD Pharmingen), Sca1 (Ly-6A/E, PE-conjugated; eBioscience), and streptavidin (fluorescein isothiocyanate [FITC]-conjugated; BD Pharmingen). Cells were analyzed with an LSR II flow cytometer and FlowJo software. Percentage and absolute number of HPCs (Lin⁻c-kit⁺Sca1⁺ or LKS⁺) and HSCs (Lin⁻c-kit⁺Sca1⁻ or LKS⁻) were determined by flow cytometry. The numbers of HPCs and HSCs in each mouse were calculated by multiplying the total numbers of BMCs harvested from both tibias and femurs of each mouse by the frequency of each population in BMCs.

Pawar S.A., Shao L., Chang J., Wang W., Pathak R., Zhu X., Wang J., Hendrickson H., Boerma M., Sterneck E., Zhou D, & Hauer-Jensen M. (2014). C/EBPδ Deficiency Sensitizes Mice to Ionizing Radiation-Induced Hematopoietic and Intestinal Injury. PLoS ONE, 9(4), e94967.

+ Open protocol

+ Expand

Hematopoietic Stem Cell Isolation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The HSC preparation was a minor modification from previously described method.³³ In brief, the whole bone marrow cells were collected from tibias in treated mice. Bone marrow cells were stained with antibodies for the identification of HSCs (c‐Kit⁺ Sca1⁺ Lin^–), and the following antibodies were used: c‐Kit‐PE (#12‐1171‐82), Sca‐1‐FITC (#11‐5981‐82), and anti‐Lineage Antibody Cocktail comprises a mixture of PE‐Cy5‐conjugated antibodies, including anti‐B220, anti‐CD4, anti‐CD8, anti‐Gr‐1, anti‐Mac‐1, and anti‐TER119 (from eBioscience). For HSC sorting, debris, dead, and clumped cells were removed to obtain single and viable cells; then, the c‐Kit⁺ Sca1⁺ Lin^– cell population was isolated by HSC sorting; FACS analysis was performed on BD FACSMelody™ Cell Sorter.³⁴

Zeng J., Liang Y., Sun R., Huang S., Wang Z., Xiao L., Lu J., Yu H, & Yao P. (2022). Hematopoietic stem cell transplantation ameliorates maternal diabetes–mediated gastrointestinal symptoms and autism‐like behavior in mouse offspring. Annals of the New York Academy of Sciences, 1512(1), 98-113.

+ Open protocol

+ Expand

Isolation of Mouse Bone Marrow Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse bone marrow cells were isolated from 6- to 8-week-old female/male C57BL/6 or CByJ.B6-Tg(UBC-GFP)30Scha/J (UBI-GFP) animals. For enrichment of HSPCs or neutrophils, bone marrow cell suspensions were incubated with lineage cell depletion kit or neutrophil isolation kit from Miltenyi Biotec (Auburn, CA). The procedure was conducted according to the manufacturer's recommendations, with 1 modification involving addition of an extra 10 µl of anti-TER-119 (Ebioscience, #13-5921-81) to the antibody cocktail.

Parada-Kusz M., Penaranda C., Hagedorn E.J., Clatworthy A., Nair A.V., Henninger J.E., Ernst C., Li B., Riquelme R., Jijon H., Villablanca E.J., Zon L.I., Hung D, & Allende M.L. (2018). Generation of mouse-zebrafish hematopoietic tissue chimeric embryos for hematopoiesis and host-pathogen interaction studies. Disease Models & Mechanisms, 11(11), dmm034876.

+ Open protocol

+ Expand

Comprehensive Immunophenotyping by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following fluorochrome-conjugated antibodies were used for flow cytometry. Anti-CD3ε (Clone ID, 145-2C11), anti-CD5 (53-7.3), anti-CD19 (1D3), anti-B220 (RA3-6B2), anti-CD11b (M1/70), anti-CD 11c (N418), anti-NK1.1 (PK136), anti-TCRγδ (eBioGL3), anti-Gr-1 (RB6-8C5), anti-FcεR1 (MAR-1), anti-CD4 (RM4-5), anti-CD8a (53-6.7), anti-CD49b (DX5), anti-TER119 (TER-119), anti-IL-7Rα (A7R34), anti-Thy1.2 (30-H12), anti-CD44 (IM7), anti-c-Kit (2B8), anti-Sca-1 (D7), anti-CD45.1 (A20), anti-CD45.2 (104), anti-IL-13 (eBio13A), anti-IFN-γ (XMG1.2), anti-IL-5 (TRFK5), anti-IL-17A (eBio17B7), anti-RORγt (AFKJS-9) and anti-T-bet (4B10) antibodies were from eBioscience. Anti-KLRG1 (2F1), anti-IL-4 (11B11), anti-Ki67 (B56) and anti-GATA-3 (L50-823) antibodies were from BD Biosciences. Anti-ST2 antibody (DJ8) was from MD Bioproducts. Anti-IL-17RB antibody (752101) was from R&D Systems. recombinant IL-25 and IL-33 were from R&D Systems. For in vitro cell culture, recombinant IL-2, IL-7, IL-3, SCF, IL-4, IL-12, IL-1β, IL-6, IL-23 and TGF-β were from Peprotech or R&D Systems, and neutralizing antibodies, including anti-IL-4 (11B11), anti-IL-12 (C17.8) and anti-IFN-γ (XMG1.2), were from Harlan Laboratories. LIVE/DEAD fixable dead cell stain kit was from Life Technologies.

Huang Y., Guo L., Qiu J., Chen X., Hu-Li J., Siebenlist U., Williamson P.R., Urban JF J.r, & Paul W.E. (2014). IL-25-responsive, lineage-negative KLRG1hi cells are multipotential “inflammatory” type 2 innate lymphoid cells. Nature immunology, 16(2), 161-169.

+ Open protocol

+ Expand

Murine Malaria Infection Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Parasites were maintained as frozen stocks and passaged in wild type mice. For the analysis of B cell responses, 6–12 weeks old mice were injected intraperitoneally (i.p.) with 10⁵Pc infected erythrocytes from a donor mouse. In tumor experiments, 6–8 week old mice were used for primary infection, and secondary and tertiary infections were performed at 8 weeks intervals using the same dose and route. Parasitemia was monitored by flow cytometry after staining 1 µl of blood with anti-Ter119 (eBiosciences) and Hoechst33342 (Invitrogen) for 30 minutes at room temperature, and confirmed by blood smear analysis.

Robbiani D.F., Deroubaix S., Feldhahn N., Oliveira T.Y., Callen E., Wang Q., Jankovic M., Silva I.T., Rommel P.C., Bosque D., Eisenreich T., Nussenzweig A, & Nussenzweig M.C. (2015). Plasmodium Infection Promotes Genomic Instability and AID Dependent B Cell Lymphoma. Cell, 162(4), 727-737.

+ Open protocol

+ Expand

Erythroblast Cell and Nuclear Size Analyses

Check if the same lab product or an alternative is used in the 5 most similar protocols

For analyses of cell and nuclear size, erythroblasts were stained with anti-CD71 (eBioscience), anti-Ter119 (eBioscience) and DRAQ5 (eBioscience) and run on the ImageStream X (Amnis). The data was analyzed with IDEAS 6.0 software (Amnis) as previously published. (Malik et al., 2015 ) Additional details on FACS analyses are in supplemental methods.

Malik J., Lillis J.A., Couch T., Getman M, & Steiner L.A. (2017). The Methyltransferase Setd8 is Essential for Erythroblast Survival and Maturation. Cell reports, 21(9), 2376-2383.

+ Open protocol

+ Expand

Flow Cytometry Analysis of Gli1+ Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flow cytometry was performed on a FACS Beckman analyzer. All data were analyzed using FlowJo software. Digested chondrogenic cells were re-suspended in FACS buffer (2%FBS in PBS) and stained with anti-CD45 (1:1000, eBioscience), anti-CD31 (1:1000, eBioscience), anti-Ter119 (1:1000, eBioscience), anti-CD90.2 (1:500, Biolegend, 105335), anti-CD51 (1:100, eBioscience, 13-0512-82), anti-CD200 (1:100, eBioscience, MA5-17980), anti-CD105 (1:100, eBioscience,17-1051-80) primary antibody for 1 h at 4 °C, following by Streptavidin, Pacific Blue™ conjugate (1:200, Thermo, S11222) for 30 min at 4 °C. After PBS wash, cells were analyzed by flow cytometry for Gli1⁺ (CD45⁻CD31⁻Ter119⁻tdTomato⁺) and Gli1⁻ (CD45⁻CD31⁻Ter119⁻tdTomato⁻) cells. Data were analyzed with FlowJo version 10.

Li B., Yang P., Shen F., You C., Wu F., Shi Y, & Ye L. (2024). Gli1 labels progenitors during chondrogenesis in postnatal mice. EMBO Reports, 25(4), 1773-1791.

+ Open protocol

+ Expand

Analyzing Bone Marrow Cell Populations

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole bone marrow cells were flushed using Hank’s balanced salt solution (HBSS) (Mg²⁺ and Ca²⁺ plus) and digested with 200 U/mL DNase I and 250 μg/mL Collagenase I and IV (Worthington) at 37°C for 30 min. Cell suspension in 100 μL HBSS with 2% bovine serum was stained with antibodies, including anti-LEPR-biotin (1:100, R&D Systems, BAF497), anti-CD45 (1:200, eBioscience, 30F-11), anti-CD31 (1:200, eBioscience, 390), anti-TER119 (1:200, eBioscience, TER-119), anti-SCA-1 (1:200, eBioscience, E13–161.7), and/or anti-PDGFRα-biotin (1:100, BioLegend, APA5), and then brilliant violet 421 streptavidin (1:500, BioLegend). Samples were analyzed using a FACSCelesta flow cytometer (BD Biosciences). Data were analyzed by FlowJo software.
For BrdU incorporation assays, mice were given an intraperitoneal injection of 1 mg BrdU (Sigma) per 6 g of body mass in PBS and maintained on 1 mg/mL of BrdU in the drinking water for 14 days. The frequency of BrdU⁺ cells was then analyzed by flow cytometry using the APC BrdU Flow Kit (BD Biosciences).
For apoptotic cell staining, cells were stained by anti-Annexin V antibody before flow cytometry following the manufacture's introductions (Thermo Fisher).

He D.D., Tang X.T., Dong W., Cui G., Peng G., Yin X., Chen Y., Jing N, & Zhou B.O. (2020). C-KIT Expression Distinguishes Fetal from Postnatal Skeletal Progenitors. Stem Cell Reports, 14(4), 614-630.

+ Open protocol

+ Expand

Sorting and Analyzing Epithelial and Stromal Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Epithelial cells from WT, LApc, and LApcL mice and stromal cells from Apc^Min and VApc^MinL mice were sorted on BD Influx (BD Biosciences) for scRNA-seq analysis and gene expression validation. The epithelial cells were surface-stained with the following antibodies at 1:500 dilution for 30 min on ice: anti-Mo CD326 (EpCAM) phycoerythrin (eBioscience; Invitrogen, #12-5791-82), CD16/CD32 (mouse BD Fc block; BD Pharmingen, #553141), anti-CD45 (eBioscience, #48-0451-82), and anti–Ter-119 (eBioscience, #48-5921-82). Sorted cells were resuspended into Hanks’ balanced salt solution with 0.04% BSA.

Heino S., Fang S., Lähde M., Högström J., Nassiri S., Campbell A., Flanagan D., Raven A., Hodder M., Nasreddin N., Xue H.H., Delorenzi M., Leedham S., Petrova T.V., Sansom O, & Alitalo K. (2021). Lef1 restricts ectopic crypt formation and tumor cell growth in intestinal adenomas. Science Advances, 7(47), eabj0512.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!