Horseradish peroxidase hrp conjugated goat anti mouse iga

Spodoptera frugiperda Sf9 insect cells were used for production of recombinant baculovirus (rBV) and VLPs in serum-free SF900 II medium (Invitrogen, Carlsbad, CA, USA). VLPs were generated by infecting Sf9 cells with recombinant baculovirus (rBV) at multiplicity of infection (MOI) of 0.05. [10 (link),11 (link)]. Madin-Darby canine kidney (MDCK) cells were grown and maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Welgene, Daegu,Korea) supplemented with 10% fetal bovine serum, 1% penicillin and streptomycin. Avian influenza viruses A/Viet Nam/1203/2004 (rgH5N1) were propagated as previously described [12 (link)]. Inactivation of the purified virus was performed by mixing the virus with formalin at a final concentration of 1:4000 (v/v) as described previously [13 (link)]. Seven-weeks-old female BALB/c mice were purchased from KOATECH (Pyeongtaek, Korea). Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgA, IgG, IgG1 and IgG2a antibodies were purchased from Southern Biotech (Birmingham, AL, USA).

H₂O₂ and CT were obtained from Sigma-Aldrich (St. Louis, MI, USA). Anti-mouse CD3-APC, CD4-FITC, CD8-PE, CD11c-eFluor 450, MHCII-APC, CD5-FITC and B220-PerCP-eFluor™ 710 antibodies were bought from eBioscience (San Diego, CA, USA). Horseradish Peroxidase (HRP)-conjugated goat anti-mouse IgA, HRP-conjugated goat anti-mouse IgG, HRP-conjugated goat anti-mouse IgG1 and HRP-conjugated goat anti-mouse IgG2a were obtained from Southern Biotech (Birmingham, AL, USA). RNA-easy Isolation was purchased from Vazyme Biotech Co., Ltd. (Nanjing, Jiangsu Province, China). SYBR qPCR Master Mix for quantitative real-time PCR (qRT-PCR) was bought from Yeasen Biotechnology Co., Ltd. (Shanghai, China). TMB was purchased from Beyotime Biotechnology (Shanghai, China). Red blood cell lysis buffer was purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). Silane coupling agent (KH550) and ZnONPs (purity 99.9%) were the products of Sinopharm Chemical Reagent Ltd. (Shanghai, China). The purified AMP was prepared in our laboratory (Yangzhou, Jiangsu Province, China). H₂SO₄, alcohol and HCl were obtained from Hushi Laboratory Equipment Co., Ltd. (Shanghai, China). Elisa plate was bought from cellpro biotechnology Co., Ltd. (Suzhou, Jiangsu Province, China).