Epidemiological data collected included gender, age, blood pressure, underlying dermatologic diseases, other underlying diseases, body surface area involvement, sensitive area involvement, topical corticosteroid potency, amount and duration of topical corticosteroids use, symptoms of AI and the presence of Cushingoid features. Biochemical data included serum cortisol at 8 AM, 0 (basal cortisol) and at 20 and 40 minutes after ACTH intravenous injection, serum creatinine, electrolytes and albumin. Serum cortisol levels were measured by electrochemiluminescence assay (ECLIA) (Elecsys® Cortisol II assay, Roche Diagnostics GmbH, Mannheim, Germany).
Elecsys cortisol 2 assay
Manufactured by Roche
Sourced in Germany, United Kingdom
The Elecsys Cortisol II assay is an in vitro diagnostic test used to quantitatively measure cortisol levels in human serum and plasma samples. The assay is designed to be performed on Roche's automated immunoassay analyzers.
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Lab products found in correlation
11 protocols using elecsys cortisol 2 assay
1
Epidemiological and Biochemical Assessment of Topical Corticosteroid-Induced Cushing's Syndrome
2
Thyroid and Cortisol Hormone Measurement
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Serum FT3 (normal reference range 2.04–4.40 pg/mL), FT4 (normal reference range 0.93–1.71 ng/dL), and TSH (normal reference range 0.27–4.2 μIU/mL) were measured by electrochemiluminescence assay (ECLIA) (Elecsys® FT3 III, FT4 III, TSH assay, Roche Diagnostics GmbH, Mannheim, Germany) with intra- and inter-assay variation of 1.9–8.2%. Serum cortisol levels were measured by electrochemiluminescence assay (ECLIA) (Elecsys® Cortisol II assay, Roche Diagnostics GmbH, Mannheim, Germany). The intra- and inter-assay coefficients of variation for serum cortisol were <10%.
3
Cortisol and ACTH Immunoassay Validation
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Cortisol was determined using an immunoassay (Elecsys Cortisol II assay, Cobas e411 analyzer, Roche Diagnostics GmbH, Mannheim, Germany), with intra- and inter-assay CV of 1.8–7.1% and 2.7–12.7%, respectively. ACTH was also determined using an immunoassay (Elecsys ACTH assay, Cobas e411 analyzer, Roche Diagnostics GmbH, Mannheim, Germany) with intra- and inter-assay CV of 2.0–2.9% and 2.4–5.4%, respectively.
4
Adrenal Hormone Assay Protocol
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PAC assay was accomplished using direct ELISA assays (DiaMetra Ekit, Spello, Italy) with a reference range in the upright position of 3–40 ng/dL and in the supine position of 2–18 ng/dL. The intra-assay variability was < 9.7%, and the inter-assay variability was < 11%. PRA was determined using a direct ELISA assay (DRG Instruments GmBH, Germany) with a reference range of 0.06–4.69 ng/mL/hr. Serum cortisol levels were measured by electrochemiluminescence assay (ECLIA) (Elecsys®, Cortisol II assay, Roche Diagnostics GmbH, Mannheim, Germany).
5
Measuring Serum Cortisol Levels with ECLIA
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Routine cortisol measurement was done using the conventional cortisol assay (Roche Elecsys® cortisol II assay, Roche Diagnosis). This widely used assay uses electrochemiluminescence immunoassay (ECLIA), which uses monoclonal assay to measure serum cortisol levels. This method can be traced to a gas chromatography-mass spectrometry/mass spectrometry (GC–MS/MS) reference40 (link),41 (link). Serum cortisol levels ranging between 7.07 and 19.6 μg/dL can be reliably measured using this assay42 .
6
Low-Dose ACTH Stimulation Test for Cortisol Evaluation
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The low-dose ACTH stimulation test was conducted between 0900 h and 1300 h by well-trained medical nurses. Serum total cortisol level was measured at 20 and 40 min following intravenous administration of 5 μg of ACTH, which was prepared by diluting a 250 μg ampule of ACTH with normal saline. The mixture was then transferred to 1 mL syringes, which were stored at 2–8 °C for no more than 60 days. Serum total cortisol level was determined via the electrochemiluminescence (ECLIA) method using an Elecsys® Cortisol II assay (Roche Diagnostics).
7
Comprehensive Hormonal Profiling Assay
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Serum total cortisol was assessed by electrochemiluminescence method (Elecsys Cortisol II assay, Roche Diagnostics). Serum FT4 (reference range 12-22 pmol/L), T3 (reference range 1.3-3.1 nmol/L), TSH (reference range 0.4-4 mIU/L), DHEAS (reference range: male 2.4-13.4 µmol/L, female (5-24 years) 1.8-11.0 µmol/L, female (25-45 years) 1.7-9.2 µmol/L, female (>45 years) 0.5-6.9 µmol/L) and plasma ACTH (reference range 1.6-13.9 pmol/L) were also assessed by the electrochemiluminescence method (Roche Diagnostics). IL-6 and IL-8 were analysed by cytometric bead array assay (BD Life Sciences, San Jose, CA, USA).
8
Adrenocorticotropic Hormone Stimulation Test Protocol
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Endocrine nurse specialists carried out all ASTs on a dedicated investigation unit in the hospital. The protocol for the AST included standard administration of intravenous or intramuscular injection of 250 µg of Synacthen (Atnahs Pharma UK Limited, Essex, UK) and measurement of plasma adrenocorticotrophic hormone ACTH and serum cortisol at 0 minutes and serum cortisol at 30 minutes. Patients on prednisolone were advised to omit the dose on the day of test until completion of the AST, while patients on hydrocortisone were advised to omit the preceding evening dose in addition to the morning dose on the day of test. Those on inhaled corticosteroids were also asked to omit the dose the evening before and on the day until the test was complete. Measurement of blood markers was conducted using the Elecsys Cortisol II assay (Roche Diagnostics GmbH, Mannheim, Germany) (Cobas interassay precision coefficient of variation 1.1-5.5%) for cortisol and Siemens Immulite 2000 and chemiluminescent assay (Siemens, Frimley, UK, interassay precision coefficient of variation 6.1% to 10.0%) for ACTH.
9
Cortisol Levels in Stress Disorders
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Based on psychobiological assumptions regarding HPA dysregulations in stress-related disorders [25 (link),26 (link)], as well as findings from prior studies conducted by our own workgroup [27 (link)] and other researchers [28 (link)] we posited that subgroups of patients could be identified based on their unstimulated cortisol levels. Participants were fasted from the night before the morning blood sampling. They were asked to quietly relax before sample collection. Serum cortisol levels were measured using the Elecsys Cortisol II assay (Roche Diagnostics GmbH, Mannheim, Germany) at the Global Clinical Central Lab (Yongin, South Korea). Based on the median cortisol levels, participants were divided into low- vs. high-cortisol groups for analysis. Given the lack of an absolute cutoff value for cortisol levels and the goal of maximizing statistical power by increasing the number of patients in both groups, we divided the two groups based on the median cortisol value, as we have previously done in a similar study [27 (link)].
10
Serum Cortisol Measurement in sCJD
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500 μl aliquots of sera taken from patients and controls between 10 a.m. and 4 p.m. were sent to The Doctor’s Laboratory (Sonic Healthcare Ltd., London, UK), where cortisol concentration was measured using the Elecsys Cortisol II assay (Roche: 11875116160) on a Cobase 801 module. Difference in cortisol concentration between sCJD and control donors was calculated in SPSS version 25 using a linear regression model with age and sex as covariates.
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