U251mg

Human glioblastoma U251MG and U87MG cell lines were obtained from the Cell Lines Service (Eppelheim, Germany). Cells were cultivated at 37 °C with 5% CO₂ in Dulbecco’s Modified Eagle Medium (DMEM; Gibco 31966021, Waltham, MA, USA) supplemented with GlutaMAX-1, 10% heat inactivated fetal bovine serum (FBS; Gibco 10500064) and antibiotics, 1% penicillin/streptomycin (Gibco 15140122). For all experiments, we formulated DMEM (Sigma-Aldrich D9443) with or without 0.4 mmol/L arginine (complete medium (CM) and arginine-free medium (AFM), respectively), supplemented with 5% dialyzed serum (Sigma-Aldrich F0392).

Primary memory CD4⁺ T cells and H-80 cells were maintained in RPMI 1640 medium (Cellgro; 10-040-CV) supplemented with 10% fetal bovine serum (FBS; PAA; A15-752), 1% penicillin-streptomycin (Cellgro; 30-001-CI), and 1% HEPES (Gibco; 15630-080) complete cRPMI (cRPMI). H-80 feeder cells (U-251 MG; Cell Lines Service) were maintained as a monolayer to a maximum 80% confluence. 293 cells used for virus production were maintained as a monolayer in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; 11995-065) plus 10% fetal bovine serum and 1% penicillin-streptomycin (cDMEM).