μ slides 6

Manufactured by Ibidi

The μ-Slides VI is a specialized laboratory equipment designed for cell culture and microscopy applications. The product provides a structured and organized platform for creating and maintaining cell cultures. Its core function is to facilitate the controlled growth and observation of cells within a defined environment.

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Lab products found in correlation

Lsm780 zen confocal microscope, by Zeiss (1 mentions) Hek293 cell, by American Type Culture Collection (1 mentions) Dmem with 10 fcs, by GE Healthcare (1 mentions) 35 mm glass bottom dishes, by MatTek (1 mentions)

Close spelling variants of this product

μ-Slide VI μ-Slide VI 0.4 channel slides μ-Slide VI 0.5 Glass Bottom 6-channel μ-Slide VI 0.4 IbiTreat μ-Slide VI 0.4 μ-Slide VI 0.4 flow chambers μ‐Slide VI 0.4 channels μ-Slide VI 0.5 glass bottom slide μ-Slide VI 0.4 plates μ-Slide VI 0.5 glass bottom channel slides

2 protocols using μ slides 6

Quantifying Endothelial Albumin Uptake

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Fluorescein isothiocyanate-labeled formaldehyde-denatured serum albumin (FITC-FSA; Sigma-Aldrich) was diluted to 10 μg/ml in endothelial medium and added to HSEC monolayers grown in rat-tail collagen-coated 0.4 channel μ-Slides VI (Ibidi). After 15 min, the channels were washed three times in PBS, the cells were fixed with 4% paraformaldehyde solution for 10 min, washed with PBS, and DAPI stained to identify nuclei, and FITC positivity was analyzed using a Carl Zeiss LSM780 Zen Confocal microscope (Carl Zeiss) and LSM software. Control cells were incubated with the same medium without the addition of FITC-FSA. Purity was assessed as the percentage of FITC-positive plated cells taken from six random fields.

Wadkin J.C., Patten D.A., Kamarajah S.K., Shepherd E.L., Novitskaya V., Berditchevski F., Adams D.H., Weston C.J, & Shetty S. (2017). CD151 supports VCAM-1-mediated lymphocyte adhesion to liver endothelium and is upregulated in chronic liver disease and hepatocellular carcinoma. American Journal of Physiology - Gastrointestinal and Liver Physiology, 313(2), G138-G149.

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HeLa/HEK-293 Transfection Protocol

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HeLa Kyoto (EMBL collection) or HEK-293 cells (ATCC) were seeded into 35-mm glass bottom dishes (MatTek) and cultured in DMEM with 10% FCS (PAA Laboratories) at 37 °C in a 5% CO₂ atmosphere. After 24 h, cells were transfected by a mixture of 1 ng DNA (or 0.65 ng DNA of each vector for co-transfection) and 3 μl (6 μl for co-transfection) X-treme GENE 9 transfection reagent per one dish. After 8–10 h, cell medium was replaced by fresh medium.
For reaction rate experiments, HeLa-Kyoto cells were seeded into μ-slides VI (IBIDI) and transfected 24 h later by a mixture of DNA and X-treme GENE 9 transfection reagent.

Ermakova Y.G., Bilan D.S., Matlashov M.E., Mishina N.M., Markvicheva K.N., Subach O.M., Subach F.V., Bogeski I., Hoth M., Enikolopov G, & Belousov V.V. (2014). Red fluorescent genetically encoded indicator for intracellular hydrogen peroxide. Nature communications, 5, 5222.

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