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Apc conjugated anti epcam

Manufactured by BioLegend

APC-conjugated anti-EpCAM is a laboratory reagent used to detect and measure the expression of the EpCAM (Epithelial Cell Adhesion Molecule) protein on the surface of cells. It consists of an anti-EpCAM antibody conjugated with the fluorescent dye APC (Allophycocyanin).

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2 protocols using apc conjugated anti epcam

1

Phenotyping of Cancer Stem Cells

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Cells were stained with the following antibodies from Biolegend (San Diego, CA): allophycocyanin (APC)-conjugated anti-CD44 (RRID:AB_312963), phycoerythrin (PE)-conjugated anti-CD24 (RRID:AB_314854) and APC-conjugated anti-EpCAM (RRID:AB_756081). FACS analysis of stained cells was performed using a FACSAria II cell sorter (Becton Dickinson, Franklin Lakes, NJ) as previously described (Lo et al., 2016 (link)).
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2

Multicolor Flow Cytometry Profiling

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Fluorescence-activated cell sorting (FACS) analysis was performed using a FACSAria II cell sorter (BD Biosciences, San Jose, CA, USA). Cells were stained with the following antibodies from Biolegend: V450-conjugated anti-CD44, FITC-conjugated anti-CD44, PE-conjugated anti-CD49f, APC-conjugated anti-EpCAM and PE/Cy7-conjugated anti-CD24.
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