Vettest 8008 chemistry analyzer

Manufactured by IDEXX

Sourced in United Kingdom

The VetTest 8008 Chemistry Analyzer is a laboratory instrument designed for in-house veterinary testing. It provides quantitative analysis of various analytes in biological samples, such as blood, serum, or plasma. The VetTest 8008 is capable of performing a wide range of clinical chemistry tests to support the diagnostic and monitoring needs of veterinary practices.

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8 protocols using vettest 8008 chemistry analyzer

Liver Injury Assessment Protocol

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Hepatotoxicity was assessed by quantifying the activities of serum alanine aminotransferase (ALT) using an ALT assay kit according to the manufacturer’s protocol (Vettest 8008 Chemistry Analyzer; IDEXX Lab., UK). The accumulation of collagen in the liver tissue was determined by estimating the hydroxyproline content, an amino acid characteristic of collagen. Hydroxyproline levels in mouse livers were measured using a hydroxyproline assay kit (BioVision, Milpitas, CA) according to the manufacturer’s instructions. The results are reported as milligrams of hydroxyproline per gram of wet liver tissue.

Hur W., Kang B.Y., Kim S.M., Lee G.W., Kim J.H., Nam M.K., Rhim H, & Yoon S.K. (2019). Serine Protease HtrA2/Omi Deficiency Impairs Mitochondrial Homeostasis and Promotes Hepatic Fibrogenesis via Activation of Hepatic Stellate Cells. Cells, 8(10), 1119.

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Comprehensive Canine Health Assessment

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Blood samples were collected from each dog via jugular venipuncture. For complete blood count (CBC) measurement, blood samples were collected into EDTA-containing tubes, and leukocytes, erythrocytes, and thrombocyte were counted using an automated hematology cell counter (MS9-5V; Melet Schloesing Lab, Osny France). To assess kidney, liver, and heart functions, we performed blood chemistry analysis using a bench-top dry chemistry analyzer (Vettest 8008 Chemistry Analyzer; IDEXX Lab, Chalfont St Peter, United Kingdom), in which creatinine, glucose, blood urea nitrogen (BUN), gamma-glutamyl transferase (GGT), albumin, total bilirubin, total protein (TP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatine kinase, cholesterol, and amylase levels were determined.

Choe Y.H., Hur T.Y., Lee S.L., Lee S., Lim D., Choi B.H., Jeong H., No J.G, & Ock S.A. (2022). Brachygnathia Inferior in Cloned Dogs Is Possibly Correlated with Variants of Wnt Signaling Pathway Initiators. International Journal of Molecular Sciences, 23(1), 475.

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Hematology and Serum Chemistry Analysis

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CBC was performed on an IDEXX Procyte Dx Hematology Analyzer (Westbrook, ME) within 15 minutes of blood draw according to the manufacturer’s instructions. Serum chemistry was performed on an IDEXX VetTest 8008 Chemistry Analyzer according to the manufacturer’s instructions.

VanderVeen N., Paran C., Appelhans A., Krasinkiewicz J., Lemons R., Appelman H., Doherty R., Palmer D., Ng P., Lowenstein P.R, & Castro M.G. (2014). Marmosets as a preclinical model for testing “off-label” use of doxycycline to turn on Flt3L expression from high-capacity adenovirus vectors. Molecular Therapy. Methods & Clinical Development, 1, 10-.

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Hematology and Serum Chemistry Analysis

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Plasma Ammonia and Orotate Quantification

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For blood collection, retro-orbital bleeds were performed with collection in lithium heparin microtainers (BD Diagnostics, Franklin Lake, NJ). Plasma ammonia was determined using a VetTest 8008 Chemistry analyzer (Idexx Laboratories, Westbrook, ME). Plasma orotate was measured using liquid chromatography mass spectrometry according to a previously published method [15 (link)]. For amino acid analysis of plasma and tissues, ion exchange chromatography with ninhydrin detection was performed using a Biochrom 30 Amino Acid Analyzer (Biochrom, Cambridge, UK). For tissues, amino acid concentrations were expressed as μmol / 100 gram of tissue. For tissue analysis, citrulline was removed due to tissue interference with detection.

Tarasenko T.N., Rosas O.R., Singh L.N., Kristaponis K., Vernon H, & McGuire P.J. (2015). A New Mouse Model of Mild Ornithine Transcarbamylase Deficiency (spf-j) Displays Cerebral Amino Acid Perturbations at Baseline and upon Systemic Immune Activation. PLoS ONE, 10(2), e0116594.

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Serum Biochemistry Analysis Protocol

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All serum biochemistry tests that included aspartate aminotransferase (AST), alanine transaminase (ALT), triglyceride, and cholesterol were examined through a VetTest 8008 Chemistry Analyzer (IDEXX, Seoul, Korea).

Kim S., Park S., Kim B, & Kwon J. (2016). Toll-like receptor 7 affects the pathogenesis of non-alcoholic fatty liver disease. Scientific Reports, 6, 27849.

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Obesity Biomarker Profiling Protocol

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All biochemical tests for obesity (triglyceride and total cholesterol concentrations) were performed on a Vet-Test 8008 Chemistry Analyzer from IDEXX, Seoul, South Korea. Levels of high-density lipoprotein (HDL) and lowdensity lipoprotein (LDL) were determined according to the instructions of kit manufacturer (Cell Biolabs, San Diego, CA, USA).

Kim S., Ahn S., Park J., Park C.H., Sin Y.S., Shin G., & Kwon J. (2020). Anti-adipogenic effects of viscothionin in 3T3-L1 adipocytes and high fat diet induced obesity mice. Applied Biological Chemistry, 63(1).

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Comprehensive Blood Biomarker Analysis

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Biochemical analyses were performed using a VetTest 8008 Chemistry Analyzer (IDEXX Laboratories, Inc., Atlanta, GA, USA). The collected blood samples were used to assess the plasma levels of total protein (TP), globulin (GLOB), albumin (Alb), blood urea nitrogen (BUN), creatinine (CREAT), total cholesterol (Chol), aspartate-aminotransferase (AST), alanine-aminotransferase (ALT), alkaline phosphatase (ALP), amylase (AMS), total bilirubin (TBILI), blood glucose (BG), sodium (Na), potassium (K), calcium (Ca), and phosphorus (P), using reagents and methods provided by IDEXX Laboratories, Inc., Atlanta, GA, USA.

Kouokam J.C., Lasnik A.B, & Palmer K.E. (2016). Studies in a Murine Model Confirm the Safety of Griffithsin and Advocate Its Further Development as a Microbicide Targeting HIV-1 and Other Enveloped Viruses. Viruses, 8(11), 311.

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