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Rabbit anti h3k27ac

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Rabbit anti-H3K27ac is a primary antibody that specifically recognizes the acetylation of lysine 27 on histone H3. This modification is associated with active enhancers and promoters, and is commonly used to study gene regulation and chromatin organization.

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Lab products found in correlation

Nuclear extraction kit, by Abcam (1 mentions) Rabbit anti h3k4me1, by Cell Signaling Technology (1 mentions) Mini trans blot electrophoretic transfer cell system, by Bio-Rad (1 mentions) Nextseq 500, by Illumina (1 mentions) Sonifier 250, by Emerson (1 mentions) Rat anti ha, by Roche (1 mentions) Ovation ultralow system v2 kit, by Tecan (1 mentions) Protein g dynabead, by Thermo Fisher Scientific (1 mentions) Amp pure beads, by Beckman Coulter (1 mentions) Rabbit anti h3k9me3, by Abcam (1 mentions) Rabbit anti fli1, by Abcam (1 mentions) Anti 6 his, by Abcam (1 mentions) Rabbit anti cbp, by Cell Signaling Technology (1 mentions) Anti sox2, by Abcam (1 mentions) Mouse anti ctnt, by Thermo Fisher Scientific (1 mentions) Mouse anti β actin, by Abcam (1 mentions) Mouse anti flag, by Merck Group (1 mentions) Rabbit anti acetylated lysine, by Cell Signaling Technology (1 mentions) Anti oct4, by Santa Cruz Biotechnology (1 mentions) Mouse anti gfap, by Merck Group (1 mentions)

Close spelling variants of this product

H3K27ac (82 citations) Anti-H3K27ac (34 citations) Rabbit H3K27ac (2 citations)

4 protocols using rabbit anti h3k27ac

1

Western Blot Analysis of Histone Modifications

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Nuclear extract was isolated using Nuclear Extraction Kit (Abcam, cat. no. ab113474) according to the manufacturer’s protocol. Samples and pre-stained protein markers were electrophoresed through 12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gels, and then transferred to polyvinylidene fluoride (PVDF) membranes, using the Mini Trans-Blot Electrophoretic Transfer Cell system (Bio-Rad). The membrane was then incubated overnight at 4 ℃ with primary antibodies, rabbit anti-H3K27ac (1:2000, Active Motif, Carlsbad, CA), rabbit anti-H3K4me1 (1:1000, Cell Signaling, Beverly, MA), or mouse anti-Lamin A/C (1:1000, Sigma), was diluted in 1 × phosphate-buffered saline with Tween (PBST). The membranes were incubated at room temperature for 2 h, with secondary antibodies (Thermo Fisher, anti-mouse, 1:1000 or anti-rabbit 1:1000, diluted in 1 × PBST). Proteins were detected using an enhanced chemiluminescence horseradish peroxidase (HRP) substrate detection kit (Merck Millipore) by BioSpectrum 2D Imaging System (UVP, BioSpectrum 800).
Jou Y.C., Lin G.L., Lin H.Y., Huang W.H., Chuang Y.M., Lin R.I., Chen P.C., Wu S.F., Shen C.H, & Chan M.W. (2021). Cyproheptadine, an epigenetic modifier, exhibits anti-tumor activity by reversing the epigenetic silencing of IRF6 in urothelial carcinoma. Cancer Cell International, 21, 226.
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2

ChIP Assay for Epigenomic Profiling

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ChIP assays of MSCs, SKNMC, A673 and HEK293T cells were carried out using two to five x 106 cells per sample and per epitope, following the procedures described previously.51 (link) In brief, chromatin from formaldehyde-fixed cells were fragmented to 200–700 bp with a Branson 250 sonifier. Solubilized chromatin was immunoprecipitated overnight at 4C with 3 mg of target specific antibodies (rat anti-HA (Roche), rabbit anti-FLI1 (Abcam), rabbit anti-H3K27ac (Active Motif), and rabbit anti-H3K9me3 (Abcam)). Antibody-chromatin complexes were pulled down with protein G-Dynabeads (Life Technologies), washed, and then eluted. After crosslink reversal, RNase A, and proteinase K treatment, immunoprecipitated DNA was extracted with AMP Pure beads (Beckman Coulter). ChIP DNA was quantified with Qubit. Sequencing libraries were prepared with 1–5 ng of ChIP DNA samples and input samples using the Ovation Ultralow System V2 kit (Nugen). Libraries were sequenced with single-end (SE) 50–75 cycles on an Illumina Nextseq 500 Illumina genome analyzer.
Tak Y.E., Boulay G., Lee L., Iyer S., Perry N.T., Schultz H.T., Garcia S.P., Broye L., Horng J.E., Rengarajan S., Naigles B., Volorio A., Sander J.D., Gong J., Riggi N., Joung J.K, & Rivera M.N. (2022). Genome-wide functional perturbation of human microsatellite repeats using engineered zinc finger transcription factors. Cell Genomics, 2(4), 100119.
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3

Antibody Panel for Cellular Analysis

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The following antibodies were used in this study: rabbit anti-CTCF (active motif, 61,311), mouse anti-Flag (Sigma, F1804), rabbit anti-acetylated lysine (Cell Signaling Technology, 9441S), mouse anti-β-ACTIN (Abcam, ab8226), rabbit anti-HDAC6 (Proteintech, 12,834–1-AP), rabbit anti-CBP (Cell Signaling Technology, 7389S), mouse anti-MOF (Boster, A02757), rabbit anti-H3K27ac (Active Motif, 39,133), anti-OCT4 (Santa Cruz Biotechnology, sc-5279), anti-SOX2 (Abcam, ab79351), mouse anti-cTnT (Thermo, MA5-12,960), rabbit IgG (Santa Cruz Biotechnology, sc-2027), and anti-6 × HIS (Abcam, ab18184).
Gong S., Hu G., Guo R., Zhang J., Yang Y., Ji B., Li G, & Yao H. (2022). CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells. Cell Regeneration, 11, 34.
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4

Western Blotting of OPCs and Brain Tissues

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For Western blotting, acutely isolated mouse OPCs or brain tissues were lysed in radioimmunoprecipitation assay buffer, containing protease and phosphatase inhibitors. Western blot analysis was performed as described previously. Mouse anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Millipore MAB374) was used as an input control. The antibodies used were mouse anti-EZH1 (Sigma-Aldrich, ABE281), rabbit anti-SUZ12 (Cell Signaling Technology, 13701S), rabbit anti-H3k27ac (Active Motif, 39135), rabbit anti-H3k27me3 (Cell Signaling Technology, 9733), rabbit anti-EZH2 (Cell Signaling Technology, 5246), rabbit anti-EED (EMD Millipore, 17-0034), goat anti-MBP (Santa Cruz Biotechnology, sc-13914), mouse anti-GFAP (Sigma-Aldrich, G3893), mouse anti-p53 (Santa Cruz Biotechnology, sc-126), and rabbit anti-p16Ink4a (Santa Cruz Biotechnology, sc-467). Secondary antibodies conjugated to horseradish peroxidase were from Jackson ImmunoResearch Laboratories.
Wang J., Yang L., Dong C., Wang J., Xu L., Qiu Y., Weng Q., Zhao C., Xin M, & Lu Q.R. (2020). EED-mediated histone methylation is critical for CNS myelination and remyelination by inhibiting WNT, BMP, and senescence pathways. Science Advances, 6(33), eaaz6477.
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